Factors associated with negative pleural adenosine deaminase results in the diagnosis of childhood pleural tuberculosis.

BACKGROUND: Until now, the influential factors associated with pleural adenosine deaminase (ADA) activity among children remain unclear. This retrospective study was therefore conducted aiming to investigate the factors associated with negative pleural ADA results in the diagnosis of childhood pleural tuberculosis (TB).
METHODS: Between January 2006 and December 2019, children patients with definite or possible pleural TB were recruited for potential analysis. Then, patients were stratified into two categories: negative pleural ADA results group (experimental group, ≤40 U/L) and positive pleural ADA results group (control group, > 40 U/L). Univariate and multivariate logistic regression analyses were performed to estimate risk factors for negative pleural ADA results.
RESULTS: A total of 84 patients with pleural TB were recruited and subsequently classified as experimental (n = 17) and control groups (n = 67). Multivariate analysis (Hosmer-Lemeshow goodness-of-fit test: χ2 = 1.881, df = 6, P = 0.930) revealed that variables, such as chest pain (age-adjusted OR = 0.0510, 95% CI: 0.004, 0.583), pleural total protein (≤45.3 g/L, age-adjusted OR = 27.7, 95% CI: 2.5, 307.7), pleural lactate dehydrogenase (LDH, ≤505 U/L, age-adjusted OR = 59.9, 95% CI: 4.2, 857.2) and blood urea nitrogen (≤3.2 mmol/L, age-adjusted OR = 32.0, 95% CI: 2.4, 426.9), were associated with negative pleural ADA results when diagnosing childhood pleural TB.
CONCLUSION: Our findings demonstrated that chest pain, pleural total protein, pleural LDH, and blood urea nitrogen were associated with a negative pleural ADA result for the diagnosis of pleural TB among children. When interpreting pleural ADA levels in children with these characteristics, a careful clinical assessment is required for the pleural TB diagnosis.

Introduction

Childhood tuberculosis (TB) remains a serious health threat. In 2018, the World Health Organization (WHO) estimated that childhood TB comprised nearly 11% of all TB cases worldwide [1]. Moreover, childhood TB appears to be increasingly reported as a cause or comorbidity of acute pneumonia [2-4]. Pleural involvement is a common form of childhood TB. Although several reports have summarized the clinical characteristics of childhood pleural TB [5-8], the diagnosis of childhood pleural TB remains a challenge. For example, Cruz AT et al. found that pleural fluid cultures for TB were positive in 56% of enrolled childhood TB cases, and no case had acid-fast bacilli (AFB) smear-positive pleural fluid; in a previous study, we found 5.4% of children with pleural TB were AFB smear-positive, 14.3% were PCR positive, and 36.6% were culture-positive [7]. Although thoracoscopy has been proven to be a sensitive and safe tool for the detection of childhood pleural TB, it has limited usefulness due to a invasive procedure [9]. In a word, the diagnostic performance of routine TB assays remains unsatisfied. In addition, some novel TB assays, such as Xpert, also have relatively limited clinical use in the diagnosis of childhood pleural TB [10, 11].

Until now, several meta-analyses have investigated the diagnostic role of pleural adenosine deaminase (ADA) in the diagnosis of pleural TB in adults, with an approximately sensitivity and specificity of 92–93% and 90–92%, respectively [12-15]. Likewise, an increased level of pleural ADA is also considered as a diagnostic criteria of childhood pleural TB. Unfortunately, several factors were reported to have an influential effect on the level of pleural ADA, such as IgG4-related pleuritis, lymphoma, age, empyema and mycobacterial load [16-20]. However, until now, the influential factors associated with pleural ADA activity in childhood pleural TB remain unclear. To improve the usefulness of pleural ADA in childhood TB, this retrospective study was therefore conducted aiming to investigate the factors associated with negative pleural ADA results for the diagnosis of childhood pleural TB.

Patients and methods

This study was conducted in accordance with the Helsinki Declaration and approved by the Ethics Committee of Shandong Provincial Chest Hospital. Due to the retrospective nature of this investigation and the anonymous nature of the data collection, written informed consent was waived by the Ethics Committee of Shandong Provincial Chest Hospital.

Between January 2006 and December 2019, children patients (≤ 15 years old) with suspected of pleural TB were recruited for potential analysis. Definite pleural TB was defined as the isolation of TB strains from mycobacterial cultures (sputum, pleural effusion, or pleural tissue), or the presence of pathological evidence (such as caseous necrosis, or Langhans’ giant cells). Possible pleural TB was diagnosed based on compatible clinical symptoms plus a positive result of TB assays (such as TB RT-PCR, acid-fast bacilli (AFB) smear, or both).

Pleural effusion were collected for analysis before starting anti-TB treatment or within 7 days of starting anti-TB treatment. The pleural ADA activity was measured colorimetrically using an ADA assay kit (Maccura, Chengdou, China) on a chemistry analyzer. The threshold of pleural ADA for childhood pleural TB was selected based on general expert opinions and most studies [13, 21, 22] and patients were then stratified into two categories: negative pleural ADA results group (referred as the experimental group, ≤40 U/L) and positive pleural ADA results group (referred as the control group, > 40 U/L). The demographic, clinical, laboratory, and radiographic features were collected from the electric medical records retrospectively.

SPSS version 16.0 (SPSS, Chicago, IL, USA) was used to perform the statistical analysis. All data were described as mean ± standard deviation (SD). Differences between the two groups were compared using Mann-Whitney U test or t test for continuous and χ2 test or Fisher exact test for categorical variables. The associations between the parameters were analyzed using the Spearman correlation test. Univariate logistic regression analysis was performed to estimate risk factors for negative pleural ADA results, and variables with P value < 0.1 were included for multivariate logistic regression analysis. Prior to multivariate regression analysis, continuous variables were transformed into categorical variables by receiver operating characteristic curve (ROC) analysis. Then, multivariate logistic regression analysis was performed and the corresponding odds ratios (OR) and 95% confidence interval (CI), adjusted by age were calculated [23]. In addition, the accuracy of the multivariate model was tested using the Hosmer-Lemeshow goodness-of-fit test. A P value < 0.05 was considered significant for the difference assessed.

Results

Characteristics of enrolled patients

During the study period, 1577 childhood TB patients were diagnosed in the center. Of them, 458 patients (29.0%) were diagnosed as pleural TB and 154 patients were confirmed as definite or possible pleural TB. Among the 154 patients with definite or possible pleural TB, 84 patients underwent pleural ADA assay, including definite (n = 69) or possible (n = 15) cases. Subsequently, these cases were classified as experimental (n = 17) and control groups (n = 67). Pathological evidence for TB were found in 27 cases (experimental, n = 5; control, n = 21) and microbiological diagnostic tests were as follows: mycobacterial culture (experimental, n = 14; control, n = 51), AFB (experimental, n = 1; control, n = 4), and TB RT-PCR (experimental, n = 8; control, n = 22). The experimental group consisted of 14 definite patients and 3 possible patients, and the control group consisted of 55 definite patients and 12 possible patients. The demographic data and clinical characteristics of children with pleural TB were presented in Table 1 and Supplementary Table 1. The mean age was 12.0 ± 3.3 years. Boys accounted for 66.7% (56 patients) and 53 (100%) were HIV-negative. The mean weight was 44.7 ± 15.7 Kg. Among the 84 children patients, 40 (47.6%) were from rural areas. The vital signs were as follows: blood pressure, 110.3 ± 12.6/68.5 ± 8.8 mmHg; heart rate, 98.4 ± 14.4 beats/min; respiratory rate, 22.5 ± 2.7 breaths/min; and temperature, 37.4 ± 1.0 °C.

Table 1

Univariate analysis of the demographic and clinical data associated with negative pleural ADA results in childhood pleural TB

	Total (n)	Pleural ADA (≤40 U/L)	Pleural ADA (>40 U/L)	P value	OR (95% CI)
N	84	17 (20.2%)	67 (79.8%)
Pleural ADA (U/L)	60.3 ± 28.8	28.3 ± 9.1	68.0 ± 26.3
Demographic characteristics
Age (years)	12.0 ± 3.3	12.1 ± 2.7	12.0 ± 3.4	0.895
Sex (male)	56 (66.7%)	11 (64.7%)	45 (67.2%)	0.848
Weight (Kg)	44.7 ± 15.7	42.3 ± 13.0	45.3 ± 16.3	0.509
Rural area	40 (47.6%)	10 (58.8%)	39 (58.2%)	0.114
Symptoms
Cough	47 (56.0%)	11 (64.7%)	36 (53.7%)	0.418
Fever	76 (90.5%)	13 (76.5%)	63 (94.0%)	0.040	4.846 (1.072, 21.916)
Chest pain	43 (51.2%)	13 (76.5%)	30 (44.8%)	0.026	0.249 (0.074, 0.845)
Dyspnea	24 (28.6%)	3 (17.6%)	21 (31.3%)	0.272
Sputum production	14 (16.7%)	2 (11.8%)	12 (17.9%)	0.547
Cavity	1 (1.2%)	0 (0.0%)	1 (1.5%)	1.000
Loculated effusion	14 (16.7%)	4 (23.5%)	10 (14.9%)	0.399
Empyema	10 (11.9%)	4 (23.5%)	6 (9.0%)	0.110
Effusion sites
Left	33 (39.3%)	10 (58.8%)	23 (34.3%)	0.071	0.366 (0.123, 1.088)
Right	43 (51.2%)	6 (35.3%)	37 (55.2%)	0.148
Both	8 (9.5%)	1 (5.9%)	7 (10.4%)	0.572
Clinical Chemistry (pleural effusion)
Total protein	48.5 ± 7.2	42.8 ± 9.0	49.9 ± 5.8	0.001	1.163 (1.061, 1.274)
Total bilirubin (mmol/L)	8.6 ± 5.6	11.3 ± 8.3	7.9 ± 4.4	0.050	0.914 (0.835, 1.000)
Glucose (mmol/L)	3.3 ± 1.5	3.9 ± 1.3	3.2 ± 1.6	0.105
Lactate dehydrogenase (U/L)	876.7 ± 642.8	480.1 ± 271.3	968.7 ± 670.3	0.002	1.004 (1.002, 1.007)
Amylase (U/L)	29.6 ± 10.4	26.6 ± 10.9	30.4 ± 10.2	0.187
Clinical Chemistry (serum)
Total protein (g/L)	69.4 ± 7.1	68.6 ± 7.8	69.5 ± 6.9	0.590
Albumin (g/L)	38.9 ± 4.4	38.8 ± 5.1	38.8 ± 4.4	0.936
Blood urea nitrogen (mmol/L)	3.9 ± 1.2	3.1 ± 0.8	4.1 ± 1.2	0.005	2.367 (1.301, 4.307)
Creatinine (μmmol/L)	51.2 ± 15.1	51.0 ± 13.2	51.5 ± 15.8	0.954
Glucose (mmol/L)	4.8 ± 0.6	4.7 ± 0.5	4.9 ± 0.7	0.412
Lactate dehydrogenase (U/L)	216.5 ± 56.1	210.5 ± 55.3	218.2 ± 56.7	0.647

ADA adenosine deaminase, TB tuberculosis, OR odds ratio, CI confidence interval

Among the enrolled children patients, 10 (11.9%) had a TB contact history and 11 (13.1%) were treated with surgical procedures. The mean transferred times between hospitals were 2.1 ± 0.9 and mean times of hospitalization were 1.8 ± 1.3. Most of them (49, 58.3%) were transferred from a teaching hospital. The symptoms complicated were as follows: fever (76, 90.5%), cough (47, 56.0%), chest pain (43, 51.2%), dyspnea (24, 28.6%), and sputum production (14, 16.7%). Radiographic findings showed that 1 patient (1.2%) had a cavity, 14 (16.7%) had loculated effusion, 33 (39.3%) had effusion on the left-side, 43 (51.2%) on the right-side, and 8 (9.5%) on the both-side. Sixty-two (73.8%) patients underwent thoracentesis before starting anti-TB treatment and 22 (26.2%) patients underwent it within seven days of starting anti-TB treatment. Out of the total of 84 study patients, 48 (57.1%) had pulmonary TB, 10 (11.9%) had empyema, 6 (7.1%) had tuberculous lymphadenitis, 2 (2.4%) had miliary TB 1 (1.2%) had bronchial TB, and 1 (1.2%) had tuberculous meningitis.

Other characteristics, such as clinical chemistry analysis (serum or pleural effusion), blood cell analysis, and flow cytometry analysis, were also showed in Table 1.

Comparisons between experimental and control groups

For comparison of continuous variables between the two groups (experimental vs control group), Mann-Whitney U tests were used and the statistical analysis showed that differences in pleural total protein (P < 0.01), pleural lactate dehydrogenase (LDH, P < 0.01), and blood urea nitrogen (P < 0.01) were significant when comparing the two groups. The differences in other continuous variables did not reach significance (all P > 0.05). For dichotomous variables, chi-square analysis was performed, and the analysis suggested that difference between the two groups was significant in fever and chest pain (all P < 0.05). The differences in other continuous variables did not reach significance (all P > 0.05).

Univariate and multivariate analysis

Univariate analysis was performed to estimate each risk factor for the negative pleural ADA results when diagnosing childhood pleural TB. It was found that pleural total protein (OR = 1.163, 95% CI: 1.061, 1.274), pleural LDH (OR = 1.004, 95% CI: 1.002, 1.007), and blood urea nitrogen (OR = 2.367, 95% CI: 1.301, 4.307), fever (OR = 4.846, 95% CI: 1.072, 21.916), and chest pain (OR = 0.249, 95% CI: 0.074, 0.845) were associated with the negative pleural ADA result in the diagnosis of childhood pleural TB (all P < 0.05).

To make the results as readily understandable as possible, continuous variables were converted into dichotomous categorical variables based on the cutoff points determined using ROC analysis, and the corresponding optimal cutoff values were 45.3 g/L, 505 U/L, and 3.2 mmol/L for pleural total protein, pleural LDH, and blood urea nitrogen, respectively. Further multivariate analysis (Hosmer–Lemeshow goodness-of-fit test: χ2 = 1.881, df = 6, P = 0.930) revealed that variables, such as chest pain (age-adjusted OR = 0.0510, 95% CI: 0.004, 0.583), pleural total protein (≤45.3 g/L, age-adjusted OR = 27.7, 95% CI: 2.5, 307.7), pleural LDH (≤505 U/L, age-adjusted OR = 59.9, 95% CI: 4.2, 857.2), and blood urea nitrogen (≤3.2 mmol/L, age-adjusted OR = 32.0, 95% CI: 2.4, 426.9), were associated with negative pleural ADA results in diagnosis of childhood pleural TB (Table 2).

Table 2

Age-adjusted OR for risk factors associated with negative pleural ADA results in childhood pleural TB

	Age-adjusted OR (95% CI)	P value
Chest pain	0.0510 (0.004, 0.583)	0.017
Pleural total protein (≤45.3 g/L)	27.7 (2.5, 307.7)	0.007
Pleural LDH (≤505 U/L)	59.9 (4.2, 857.2)	0.003
Blood urea nitrogen (≤3.2 mmol/L)	32.0 (2.4, 426.9)	0.009

OR odds ratio, TB tuberculosis, ADA adenosine deaminase, CI confidence interval, LDH lactate dehydrogenase

Discussion

Pleural effusion is a common complication of pneumonia in children. Pleural TB is usually considered if the pleural ADA have a value of > 40 U/L [24]. However, it remains a significant proportion of children with pleural TB have a pleural ADA value under the threshold of 40 U/L [6]. In this study, several risk factors, such as absence of chest pain and higher values of pleural total protein, pleural LDH, and blood urea nitrogen were associated with negative pleural ADA results in children with pleural TB. To our best knowledge, this study is the first research investigating the association between variables and negative pleural ADA results, and we believe that our findings would aid to improve the diagnosis of pleural TB in children.

ADA is known as an enzyme which catalyses the conversion of adenosine to inosine and joins in the differentiation of lymphoid cells. Currently, several studies have investigated the factors influencing the pleural level of ADA. First, a high ADA activity is associated with a stimulated cellular immunity. Production of pleural ADA reflects an activation of T cells and monocytes in effusion [25]. In a previous study, it was demonstrated a positive correlation between pleural ADA level and CD4 lymphocyte counts [26]. Second, Kim SB et al. found that older age was significantly associated with low pleural ADA activity among patients with pleural TB [17]. Similarly, the association between age and pleural ADA was also reported in other studies [27-29]. A possible explanation for this is that aging declines human immunity, such as T cell function, macrophage number and functions [30-33]. As the above mentioned, age-related changes in the activity of ADA may be expected. Third, a typical tuberculous pleural effusion manifestation involves lymphocyte predominance. However, up to 10% of tuberculous effusions are neutrophil-dominant pattern, which means a lower level of pleural ADA activity [34]. Fourth, pleural ADA activity may be associated with a status of anti-TB treatment. Soedarsono S et al. found that the serum ADA level at the beginning of TB treatment was higher than the level at the end of intensive phase treatment, and then the study concluded that the serum ADA test can be used to evaluate the pulmonary TB treatment response [35]. In addition, an increased level of pleural ADA levels are also found in other etiologies, such as rheumatoid pleural effusion, bacterial pleural infection, mesothelioma, lung cancer, leukaemia, empyema, and lymphoma [20, 36]. Although these factors influencing the level of pleural ADA have been identified previously, our findings are inconsistent with these findings. This may be attributed to two reasons: a small sample size and a younger population recruited.

First, our findings found that the absence of chest pain is considered a risk factor of negative pleural ADA results. One possible explanation is that in a previous study, chest pain was more common in children with complicated community-acquired pneumonia and associated with post-operative death [37, 38]; in addition, several pleural effusion markers are associated with complicated effusion and serious outcomes (such as death), e.g. low pleural pH and glucose, and high pleural LDH activity [39]; therefore, it is thought that the chest pain is associated with a higher level of pleural ADA among children with pleural TB. Moreover, in terms of TB disease, chest pain was associated with the occurrence of pleural TB [40].

Second, a decreased level of blood urea nitrogen was associated with a negative pleural ADA result. This maybe inconsistent with the previous findings in adulthood [41]. Because, In adult patients with renal failure, haemodialysis is a confounding factor which could reduce the levels of ADA [42]. In fact, the exact mechanism of the association between blood urea nitrogen and pleural ADA in children patients remains unclear. This may be explained by a positive correlation between age and blood urea nitrogen [43]. However, to illustrate the point, further investigation is required.

Third, pleural protein and LDH indicated a degree of pleural inflammation, it is thought that a greater pleural inflammation would lead to more activated lymphocytes and ADA production. Therefore, a positive association is built between pleural ADA and other inflammatory biomarkers. For example, a previous study suggested a significant correlation between pleural ADA and pleural protein and LDH [29]. In addition, Bielsa S et al. found that pleural ADA < 35 U/L was associated with pleural LDH levels < 500 U/L [44]. These finding are similar to our observation of the study.

Our study also had some limitations. First, the ADA criteria (40 U/L) may be high for the diagnosis of pleural TB in areas with high TB prevalence. Second, this was a single centre study, it thus may only reflect a local epidemiological situation. Third, the study had a retrospective nature and some clinical data (e.g., pleural cell subsets, isoenzyme activity (ADA1 and ADA2)) could not be obtained. Therefore, further studies are needed to determine the mechanisms involving low ADA activity among patients with pleural TB, and future large prospective studies would be needed to validate the above findings.

Conclusions

The study found that several variables, such as chest pain, pleural total protein, pleural LDH, and blood urea nitrogen, have been identified as risk factors associated with negative pleural ADA results in the diagnosis of pleural TB among children. A false pleural ADA result may lower the suspicion of pleural TB and result in delayed diagnosis and anti-TB treatment. Therefore, when interpreting pleural ADA levels in children with such characteristics for the diagnosis of pleural TB, a careful clinical assessment is required.

Additional file 1.