Malek-Hosseini Saeed1, Kalantar Kurosh1, Amirghofran Zahra1,2, Dabbaghmanesh Mohamad Hossein3, Rostamzadeh Davood1, Mohammad Reza Ataollahi4. 1. Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. 2. Autoimmune Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 3. Endocrine and Metabolism Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran. 4. Department of Immunology, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran, ataollahimr@gmail.com.
Abstract
OBJECTIVE: Graves' disease (GD) is an autoimmune disease causing the overproduction of the thyroid hormone from thyroid gland. This disease is mainly the result of the production of antibodies against TSH receptors. Cytokines play an important role in orchestrating the pathophysiology in autoimmune thyroid disease. The regulatory role of IL-12 on TH1 cells has been proven. IL-27 and IL-35, members of IL-12 cytokine family, are two cytokines that have been newly discovered. IL-35 has been identified as a novel immunosuppressive and anti-inflammatory cytokine while IL-27 has both inflammatory and anti-inflammatory functions. The objective of the current study was to examine the changes in the serum level of the foregoing cytokines in GD patients in comparison to healthy controls. METHODS: In this study, serum levels of IL-27 and IL-35 were determined by an ELISA method; anti TPO and anti Tg were measured by an RIA method in 40 new cases of Graves's disease. The findings were compared with 40 healthy controls. RESULTS: The results showed a significant difference between IL-27 and IL-35 regarding their serum levels with P values of 0.0001 and 0.024, respectively; anti TPO and anti Tg levels of the cases were also significantly different from controls (p < 0.001). CONCLUSION: The reduction in the serum levels of IL-27 and IL-35 in GD patients compared to normal subjects suggests the possible anti-inflammatory role of these cytokines in GD.
OBJECTIVE: Graves' disease (GD) is an autoimmune disease causing the overproduction of the thyroid hormone from thyroid gland. This disease is mainly the result of the production of antibodies against TSH receptors. Cytokines play an important role in orchestrating the pathophysiology in autoimmune thyroid disease. The regulatory role of IL-12 on TH1 cells has been proven. IL-27 and IL-35, members of IL-12 cytokine family, are two cytokines that have been newly discovered. IL-35 has been identified as a novel immunosuppressive and anti-inflammatory cytokine while IL-27 has both inflammatory and anti-inflammatory functions. The objective of the current study was to examine the changes in the serum level of the foregoing cytokines in GD patients in comparison to healthy controls. METHODS: In this study, serum levels of IL-27 and IL-35 were determined by an ELISA method; anti TPO and anti Tg were measured by an RIA method in 40 new cases of Graves's disease. The findings were compared with 40 healthy controls. RESULTS: The results showed a significant difference between IL-27 and IL-35 regarding their serum levels with P values of 0.0001 and 0.024, respectively; anti TPO and anti Tg levels of the cases were also significantly different from controls (p < 0.001). CONCLUSION: The reduction in the serum levels of IL-27 and IL-35 in GD patients compared to normal subjects suggests the possible anti-inflammatory role of these cytokines in GD.
Authors: Gökcen Eraslan; Eugene Drokhlyansky; Shankara Anand; Evgenij Fiskin; Ayshwarya Subramanian; Michal Slyper; Jiali Wang; Ayellet V Segrè; François Aguet; Orit Rozenblatt-Rosen; Kristin G Ardlie; Aviv Regev; Nicholas Van Wittenberghe; John M Rouhana; Julia Waldman; Orr Ashenberg; Monkol Lek; Danielle Dionne; Thet Su Win; Michael S Cuoco; Olena Kuksenko; Alexander M Tsankov; Philip A Branton; Jamie L Marshall; Anna Greka; Gad Getz Journal: Science Date: 2022-05-13 Impact factor: 63.714