Antiosteoporotic effect of hesperidin against ovariectomy-induced osteoporosis in rats via reduction of oxidative stress and inflammation.

Osteoporosis is a serious health problem, especially in geriatric patients. Worldwide, it affects 8.9 million people every year. Oxidative stress and inflammation expand the osteoporosis reaction. Hesperidin supplement helps to decrease inflammation and oxidative stress. In this study, we estimated the antiosteoporotic effect of hesperidin against the ovariectomized (OVX) rat model of osteoporosis. Hesperidin was orally administered at 5, 10, and 20 mg/kg to OVX rats for 10 weeks. Different biochemical parameters, such as alkaline phosphatase (ALP), osteocalcin (OC), phosphorus (P), calcium (Ca), and antioxidant parameters, were also estimated. The three-point bending test, bone mineral density (BMD), and histomorphometric features of the femoral bone were also scrutinized. Hesperidin significantly decreased body weight and increased uterine weight. Hesperidin significantly reduced the ALP, OC, acid phosphatase, and β-isomerized C-terminal telopeptides levels in OVX rats. Hesperidin considerably increased BMD and dose-dependently reduced the pixel density. Hesperidin considerably increased the maximum load, energy, stiffness, maximum stress, and young modulus. Hesperidin significantly (p < 0.001) reduced the levels of thiobarbituric acid reactive substance and increased the level of superoxide dismutase, glutathione, glutathione peroxidase, catalase in OVX-induced rats. Hesperidin significantly diminishes the cytokine levels, such as tumor necrosis factor-α, interleukin-6 (IL-6), and IL-1β, and inflammatory mediators such as nuclear factor-kappa B. It significantly reduced the level of Ca, P, and increased the level of vitamin D in OVX rats. Hesperidin significantly (p < 0.001) reduced the expression of sirtuin 1. Collectively, we can conclude that hesperidin exhibited better protection against osteoporosis by enhancing the bone density and bone mineral content in addition to biomechanical parameters.