Literature DB >> 34028726

FLIM Imaging for Metabolic Studies in Live Cells.

Heejun Choi1.   

Abstract

Fluorescent biochemical sensors allow probing metabolic states in a living cell with high spatiotemporal dynamics. This chapter describes a method for the in situ detection of changes in NAD+ level in living cells using fluorescence lifetime imaging (FLIM).

Entities:  

Keywords:  Biosensors; Cytosolic NAD+; FLIM; FRET; Metabolism; Mitochondrial NAD+; NAD+; Nuclear NAD+

Year:  2021        PMID： 34028726     DOI： 10.1007/978-1-0716-1402-0_18

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

1.  In vivo multiphoton fluorescence lifetime imaging of protein-bound and free nicotinamide adenine dinucleotide in normal and precancerous epithelia.

Authors:  Melissa C Skala; Kristin M Riching; Damian K Bird; Annette Gendron-Fitzpatrick; Jens Eickhoff; Kevin W Eliceiri; Patricia J Keely; Nirmala Ramanujam
Journal:  J Biomed Opt       Date:  2007 Mar-Apr       Impact factor: 3.170

2.  Neuronal Stimulation Triggers Neuronal Glycolysis and Not Lactate Uptake.

Authors:  Carlos Manlio Díaz-García; Rebecca Mongeon; Carolina Lahmann; Dorothy Koveal; Hannah Zucker; Gary Yellen
Journal:  Cell Metab       Date:  2017-08-01       Impact factor: 27.287

3.  A dark yellow fluorescent protein (YFP)-based Resonance Energy-Accepting Chromoprotein (REACh) for Förster resonance energy transfer with GFP.

Authors:  Sundar Ganesan; Simon M Ameer-Beg; Tony T C Ng; Borivoj Vojnovic; Fred S Wouters
Journal:  Proc Natl Acad Sci U S A       Date:  2006-03-06       Impact factor: 11.205

4.  A mTurquoise-based cAMP sensor for both FLIM and ratiometric read-out has improved dynamic range.

Authors:  Jeffrey B Klarenbeek; Joachim Goedhart; Mark A Hink; Theodorus W J Gadella; Kees Jalink
Journal:  PLoS One       Date:  2011-04-29       Impact factor: 3.240

5.  Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides.

Authors:  Olivier Sallin; Luc Reymond; Corentin Gondrand; Fabio Raith; Birgit Koch; Kai Johnsson
Journal:  Elife       Date:  2018-05-29       Impact factor: 8.140

6.  Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics.

Authors:  Jasper Akerboom; Nicole Carreras Calderón; Lin Tian; Sebastian Wabnig; Matthias Prigge; Johan Tolö; Andrew Gordus; Michael B Orger; Kristen E Severi; John J Macklin; Ronak Patel; Stefan R Pulver; Trevor J Wardill; Elisabeth Fischer; Christina Schüler; Tsai-Wen Chen; Karen S Sarkisyan; Jonathan S Marvin; Cornelia I Bargmann; Douglas S Kim; Sebastian Kügler; Leon Lagnado; Peter Hegemann; Alexander Gottschalk; Eric R Schreiter; Loren L Looger
Journal:  Front Mol Neurosci       Date:  2013-03-04       Impact factor: 5.639
  6 in total

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