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Literature DB >> 34028720

High-Throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains.

Mojca Mattiazzi Usaj^1,2, Dara S Lo^1,3, Ben T Grys^1,3, Brenda J Andrews^4,5.

Abstract

We describe a protocol for live-cell high-throughput (HTP) screening of yeast mutant strains carrying fluorescent protein markers for subcellular compartments of choice using automated confocal microscopy. This procedure, which combines HTP genetics and microscopy, results in the acquisition of thousands of images that can be analyzed in a systematic and quantitative way to identify morphology defects in the tagged subcellular compartments. This HTP protocol is readily adapted for screening any combination of markers and can be expanded to different growth conditions or higher order mutant genetic backgrounds.

Entities: Chemical

Keywords: Confocal fluorescence microscopy; Genetic screening; High-content screening; High-throughput microscopy; Yeast

Mesh：

Substances：

Year: 2021 PMID： 34028720 PMCID： PMC9107603 DOI： 10.1007/978-1-0716-1402-0_12

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

23 in total

Review 1. Phenotypic Image Analysis Software Tools for Exploring and Understanding Big Image Data from Cell-Based Assays.

Authors: Kevin Smith; Filippo Piccinini; Tamas Balassa; Krisztian Koos; Tivadar Danka; Hossein Azizpour; Peter Horvath
Journal: Cell Syst Date: 2018-06-27 Impact factor: 10.304

Review 2. High-Content Screening for Quantitative Cell Biology.

Authors: Mojca Mattiazzi Usaj; Erin B Styles; Adrian J Verster; Helena Friesen; Charles Boone; Brenda J Andrews
Journal: Trends Cell Biol Date: 2016-04-22 Impact factor: 20.808

3. Systematic genetic analysis with ordered arrays of yeast deletion mutants.

Authors: A H Tong; M Evangelista; A B Parsons; H Xu; G D Bader; N Pagé; M Robinson; S Raghibizadeh; C W Hogue; H Bussey; B Andrews; M Tyers; C Boone
Journal: Science Date: 2001-12-14 Impact factor: 47.728

4. Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.

Authors: Yanhui Hu; Andreas Rolfs; Bhupinder Bhullar; Tellamraju V S Murthy; Cong Zhu; Michael F Berger; Anamaria A Camargo; Fontina Kelley; Seamus McCarron; Daniel Jepson; Aaron Richardson; Jacob Raphael; Donna Moreira; Elena Taycher; Dongmei Zuo; Stephanie Mohr; Michael F Kane; Janice Williamson; Andrew Simpson; Martha L Bulyk; Edward Harlow; Gerald Marsischky; Richard D Kolodner; Joshua LaBaer
Journal: Genome Res Date: 2007-02-23 Impact factor: 9.043

5. Functional profiling of the Saccharomyces cerevisiae genome.

Authors: Guri Giaever; Angela M Chu; Li Ni; Carla Connelly; Linda Riles; Steeve Véronneau; Sally Dow; Ankuta Lucau-Danila; Keith Anderson; Bruno André; Adam P Arkin; Anna Astromoff; Mohamed El-Bakkoury; Rhonda Bangham; Rocio Benito; Sophie Brachat; Stefano Campanaro; Matt Curtiss; Karen Davis; Adam Deutschbauer; Karl-Dieter Entian; Patrick Flaherty; Francoise Foury; David J Garfinkel; Mark Gerstein; Deanna Gotte; Ulrich Güldener; Johannes H Hegemann; Svenja Hempel; Zelek Herman; Daniel F Jaramillo; Diane E Kelly; Steven L Kelly; Peter Kötter; Darlene LaBonte; David C Lamb; Ning Lan; Hong Liang; Hong Liao; Lucy Liu; Chuanyun Luo; Marc Lussier; Rong Mao; Patrice Menard; Siew Loon Ooi; Jose L Revuelta; Christopher J Roberts; Matthias Rose; Petra Ross-Macdonald; Bart Scherens; Greg Schimmack; Brenda Shafer; Daniel D Shoemaker; Sharon Sookhai-Mahadeo; Reginald K Storms; Jeffrey N Strathern; Giorgio Valle; Marleen Voet; Guido Volckaert; Ching-yun Wang; Teresa R Ward; Julie Wilhelmy; Elizabeth A Winzeler; Yonghong Yang; Grace Yen; Elaine Youngman; Kexin Yu; Howard Bussey; Jef D Boeke; Michael Snyder; Peter Philippsen; Ronald W Davis; Mark Johnston
Journal: Nature Date: 2002-07-25 Impact factor: 49.962

6. Mapping pathways and phenotypes by systematic gene overexpression.

Authors: Richelle Sopko; Dongqing Huang; Nicolle Preston; Gordon Chua; Balázs Papp; Kimberly Kafadar; Mike Snyder; Stephen G Oliver; Martha Cyert; Timothy R Hughes; Charles Boone; Brenda Andrews
Journal: Mol Cell Date: 2006-02-03 Impact factor: 17.970

7. A global genetic interaction network maps a wiring diagram of cellular function.

Authors: Michael Costanzo; Benjamin VanderSluis; Elizabeth N Koch; Anastasia Baryshnikova; Carles Pons; Guihong Tan; Wen Wang; Matej Usaj; Julia Hanchard; Susan D Lee; Vicent Pelechano; Erin B Styles; Maximilian Billmann; Jolanda van Leeuwen; Nydia van Dyk; Zhen-Yuan Lin; Elena Kuzmin; Justin Nelson; Jeff S Piotrowski; Tharan Srikumar; Sondra Bahr; Yiqun Chen; Raamesh Deshpande; Christoph F Kurat; Sheena C Li; Zhijian Li; Mojca Mattiazzi Usaj; Hiroki Okada; Natasha Pascoe; Bryan-Joseph San Luis; Sara Sharifpoor; Emira Shuteriqi; Scott W Simpkins; Jamie Snider; Harsha Garadi Suresh; Yizhao Tan; Hongwei Zhu; Noel Malod-Dognin; Vuk Janjic; Natasa Przulj; Olga G Troyanskaya; Igor Stagljar; Tian Xia; Yoshikazu Ohya; Anne-Claude Gingras; Brian Raught; Michael Boutros; Lars M Steinmetz; Claire L Moore; Adam P Rosebrock; Amy A Caudy; Chad L Myers; Brenda Andrews; Charles Boone
Journal: Science Date: 2016-09-23 Impact factor: 47.728

High-Throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains.

Review 1. Phenotypic Image Analysis Software Tools for Exploring and Understanding Big Image Data from Cell-Based Assays.

Review 2. High-Content Screening for Quantitative Cell Biology.

3. Systematic genetic analysis with ordered arrays of yeast deletion mutants.

4. Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.

5. Functional profiling of the Saccharomyces cerevisiae genome.

6. Mapping pathways and phenotypes by systematic gene overexpression.

7. A global genetic interaction network maps a wiring diagram of cellular function.

Review 8. Machine learning and image-based profiling in drug discovery.

9. A comprehensive strategy enabling high-resolution functional analysis of the yeast genome.

10. CellProfiler Analyst: data exploration and analysis software for complex image-based screens.

1. Phenomics approaches to understand genetic networks and gene function in yeast.