Etienne Gouraud1,2, Philippe Connes1,2,3, Alexandra Gauthier-Vasserot1,2,4,5, Camille Faes1,2, Salima Merazga5, Solène Poutrel5,6, Céline Renoux1,2,7, Camille Boisson1,2, Philippe Joly1,2,7, Yves Bertrand4,5, Arnaud Hot5,6, Giovanna Cannas1,2,5,6, Christophe Hautier8,9. 1. Inter-University Laboratory of Human Movement Sciences (LIBM) EA7424, Team "Vascular Biology and Red Blood Cell", University Claude Bernard Lyon 1, Villeurbanne, France. 2. Laboratory of Excellence "GR-Ex", Paris, France. 3. Institute of Universities of France, Paris, France. 4. Hematology and Oncology Pediatric Unit, University Hospital of Lyon, Lyon, France. 5. Reference Centre in Sickle Cell Disease, Thalassemia and Rare Red Blood Cell and Erythropoiesis Diseases, Hospices Civils de Lyon, Lyon, France. 6. Internal Medicine Department, Edouard-Herriot Hospital, Lyon, France. 7. Laboratory of Biochemistry of Erythrocyte Pathologies, Biology Centre East, Bron, France. 8. Inter-University Laboratory of Human Movement Sciences (LIBM) EA7424, Team "Vascular Biology and Red Blood Cell", University Claude Bernard Lyon 1, Villeurbanne, France. christophe.hautier@univ-lyon1.fr. 9. Laboratory of Excellence "GR-Ex", Paris, France. christophe.hautier@univ-lyon1.fr.
Abstract
PURPOSE: Sickle cell disease (SCD) patients exhibit a limited exercise tolerance commonly attributed to anaemia, as well as hemorheological and cardio-respiratory abnormalities, but the functional status of skeletal muscle at exercise is unknown. Moreover, the effect of SCD genotype on exercise tolerance and skeletal muscle function has been poorly investigated. The aim of this study was to investigate skeletal muscle function and fatigue during a submaximal exercise in SCD patients. METHODS: Nineteen healthy individuals (AA), 28 patients with sickle cell anaemia (SS) and 18 with sickle cell-haemoglobin C disease (SC) performed repeated knee extensions exercise (FAT). Maximal isometric torque (Tmax) was measured before and after the FAT to quantify muscle fatigability. Electromyographic activity and oxygenation by near-infrared spectroscopy of the Vastus Lateralis were recorded. RESULTS: FAT caused a reduction in Tmax in SS (- 17.0 ± 12.1%, p < 0.001) and SC (- 21.5 ± 14.5%, p < 0.05) but not in AA (+ 0.58 ± 29.9%). Root-mean-squared value of EMG signal (RMS) decreased only in SS after FAT, while the median power frequency (MPF) was unchanged in all groups. Oxygenation kinetics were determined in SS and AA and were not different. CONCLUSION: These results show skeletal muscle dysfunction during exercise in SCD patients, and suggest different fatigue aetiology between SS and SC. The changes in EMG signal and oxygenation kinetics during exercise suggest that the greater skeletal muscle fatigue occurring in SCD patients would be rather due to intramuscular alterations modifications than decreased tissue oxygenation. Moreover, SS patients exhibit greater muscle fatigability than SC.
PURPOSE: Sickle cell disease (SCD) patients exhibit a limited exercise tolerance commonly attributed to anaemia, as well as hemorheological and cardio-respiratory abnormalities, but the functional status of skeletal muscle at exercise is unknown. Moreover, the effect of SCD genotype on exercise tolerance and skeletal muscle function has been poorly investigated. The aim of this study was to investigate skeletal muscle function and fatigue during a submaximal exercise in SCD patients. METHODS: Nineteen healthy individuals (AA), 28 patients with sickle cell anaemia (SS) and 18 with sickle cell-haemoglobin C disease (SC) performed repeated knee extensions exercise (FAT). Maximal isometric torque (Tmax) was measured before and after the FAT to quantify muscle fatigability. Electromyographic activity and oxygenation by near-infrared spectroscopy of the Vastus Lateralis were recorded. RESULTS: FAT caused a reduction in Tmax in SS (- 17.0 ± 12.1%, p < 0.001) and SC (- 21.5 ± 14.5%, p < 0.05) but not in AA (+ 0.58 ± 29.9%). Root-mean-squared value of EMG signal (RMS) decreased only in SS after FAT, while the median power frequency (MPF) was unchanged in all groups. Oxygenation kinetics were determined in SS and AA and were not different. CONCLUSION: These results show skeletal muscle dysfunction during exercise in SCD patients, and suggest different fatigue aetiology between SS and SC. The changes in EMG signal and oxygenation kinetics during exercise suggest that the greater skeletal muscle fatigue occurring in SCD patients would be rather due to intramuscular alterations modifications than decreased tissue oxygenation. Moreover, SS patients exhibit greater muscle fatigability than SC.
Authors: Gregory M Blain; Tyler S Mangum; Simranjit K Sidhu; Joshua C Weavil; Thomas J Hureau; Jacob E Jessop; Amber D Bledsoe; Russell S Richardson; Markus Amann Journal: J Physiol Date: 2016-07-08 Impact factor: 5.182
Authors: Anastasia Anthi; Roberto F Machado; Maria L Jison; Angelo M Taveira-Dasilva; Lewis J Rubin; Lori Hunter; Christian J Hunter; Wynona Coles; James Nichols; Nilo A Avila; Vandana Sachdev; Clara C Chen; Mark T Gladwin Journal: Am J Respir Crit Care Med Date: 2007-03-22 Impact factor: 21.405
Authors: Markus Amann; Massimo Venturelli; Stephen J Ives; John McDaniel; Gwenael Layec; Matthew J Rossman; Russell S Richardson Journal: J Appl Physiol (1985) Date: 2013-05-30