Ilias Giannenas1, Katerina Grigoriadou2,3, Erasmia Sidiropoulou4, Eleftherios Bonos5, Antigoni Cheilari6, Argyro Vontzalidou6, Chrisoula Karaiskou7, Nektarios Aligiannis6, Panagiota Florou-Paneri4, Efterpi Christaki4. 1. Laboratory of Animal Nutrition, School of Veterinary Medicine, Aristotle University, 54124, Thessaloniki, Greece. igiannenas@vet.auth.gr. 2. Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization - DEMETER, P.O. Box 60458, Thermi, 570 01, Thessaloniki, Greece. 3. ELVIZ Hellenic Feedstuff Industry S.A, 59300, Plati-Imathia, Greece. 4. Laboratory of Animal Nutrition, School of Veterinary Medicine, Aristotle University, 54124, Thessaloniki, Greece. 5. Department of Agriculture, School of Agriculture, University of Ioannina, Kostakioi, 47100, Artas, Greece. 6. Section of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771, Athens, Greece. 7. Laboratory of Animal Husbandry, School of Agriculture, Aristotle University, 54124, Thessaloniki, Greece.
Abstract
INTRODUCTION: Bioactive constituents of medicinal-aromatic plants used as feed additives may affect the metabolic profile and oxidative stability of hen eggs. OBJECTIVES: To determine the effects of dietary supplementation with a mixture of dried oregano, thyme, sideritis tea and chamomile on laying hen performance, egg quality parameters, and oxidative stability in the egg yolk were monitored. METHODS: In this trial 432 hens were allocated in two treatments (unsupplemented vs. supplemented with the mixture) and fed for 42 days. Eggs were collected at the end of the trial period, egg yolk was separated, extracted, and the total phenolic content (TPC) and oxidative stability was measured. Furthermore, LC-MS metabolic profile of eggs was studied and pathway analysis was elaborated in MetaboAnalyst to facilitate annotation of features. RESULTS: Overall, egg production and feed conversion ratio were not affected by the supplementation. However, eggs from the supplemented treatment showed improved shell thickness and strength, and yolk resistance to oxidation. Moreover, LC-MS metabolomic analysis of egg yolk of supplemented and unsupplemented layers showed significant variations and tight clustering in unsupervised principal component analysis due to different chemical profiling of egg yolk. LC-MS study showed that secondary metabolites of aromatic plants did not transfer into yolk, nevertheless the feed supplementation impacted the pathway metabolism of tyrosine, phenylalanine, propanate, and the biosynthesis of aminoacyl-tRNA, phenylalanine, tyrosine and tryptophan. CONCLUSIONS: The dietary supplementation of layers with a mixture of dried medicinal aromatic plants affected shell thickness and strength, the lipid and protein oxidative stability and increased tyrosine and phenylalanine content in eggs.
INTRODUCTION: Bioactive constituents of medicinal-aromatic plants used as feed additives may affect the metabolic profile and oxidative stability of hen eggs. OBJECTIVES: To determine the effects of dietary supplementation with a mixture of dried oregano, thyme, sideritis tea and chamomile on laying hen performance, egg quality parameters, and oxidative stability in the egg yolk were monitored. METHODS: In this trial 432 hens were allocated in two treatments (unsupplemented vs. supplemented with the mixture) and fed for 42 days. Eggs were collected at the end of the trial period, egg yolk was separated, extracted, and the total phenolic content (TPC) and oxidative stability was measured. Furthermore, LC-MS metabolic profile of eggs was studied and pathway analysis was elaborated in MetaboAnalyst to facilitate annotation of features. RESULTS: Overall, egg production and feed conversion ratio were not affected by the supplementation. However, eggs from the supplemented treatment showed improved shell thickness and strength, and yolk resistance to oxidation. Moreover, LC-MS metabolomic analysis of egg yolk of supplemented and unsupplemented layers showed significant variations and tight clustering in unsupervised principal component analysis due to different chemical profiling of egg yolk. LC-MS study showed that secondary metabolites of aromatic plants did not transfer into yolk, nevertheless the feed supplementation impacted the pathway metabolism of tyrosine, phenylalanine, propanate, and the biosynthesis of aminoacyl-tRNA, phenylalanine, tyrosine and tryptophan. CONCLUSIONS: The dietary supplementation of layers with a mixture of dried medicinal aromatic plants affected shell thickness and strength, the lipid and protein oxidative stability and increased tyrosine and phenylalanine content in eggs.
Authors: I Giannenas; E Bonos; I Skoufos; A Tzora; I Stylianaki; D Lazari; A Tsinas; E Christaki; P Florou-Paneri Journal: Br Poult Sci Date: 2018-09-17 Impact factor: 2.095
Authors: Michael Goliomytis; Nikos Kartsonas; Maria A Charismiadou; George K Symeon; Panagiotis E Simitzis; Stelios G Deligeorgis Journal: PLoS One Date: 2015-10-28 Impact factor: 3.240