| Literature DB >> 34017000 |
Ruifeng Zhao1,2, Qisheng Zuo1,2, Xia Yuan1,2, Kai Jin1,2, Jing Jin1,2, Ying Ding1,2, Chen Zhang1,2, Tingting Li1,2, Jingyi Jiang1,2, Jiancheng Li1,2, Ming Zhang1,2, Xiang Shi1,2, Hongyan Sun1,2, Yani Zhang1,2, Qi Xu1,2, Guobin Chang1,2, Zhenhua Zhao3, Bing Li3, Xinsheng Wu1,2, Yang Zhang1,2, Jiuzhou Song4, Guohong Chen5,6, Bichun Li7,8.
Abstract
The allogeneic transplantation of primordial germ cells (PGCs) derived from somatic cells overcomes the limitation of avian cloning. Here, we transdifferentiate chicken embryo fibroblasts (CEFs) from black feathered Langshan chickens to PGCs and transplant them into White Plymouth Rock chicken embryos to produce viable offspring with characteristics inherited from the donor. We express Oct4/Sox2/Nanog/Lin28A (OSNL) to reprogram CEFs to induced pluripotent stem cells (iPSCs), which are further induced to differentiate into PGCs by BMP4/BMP8b/EGF. DNA demethylation, histone acetylation and glycolytic activation elevate the iPSC induction efficiency, while histone acetylation and glycolytic inhibition facilitate PGCs formation. The induced PGCs (iPGCs) are transplanted into the recipients, which are self-crossed to produce 189/509 somatic cells derived chicken with the donor's characteristics. Microsatellite analysis and genome sequencing confirm the inheritance of genetic information from the donor. Thus, we demonstrate the feasibility of avian cloning from somatic cells.Entities:
Year: 2021 PMID： 34017000 DOI： 10.1038/s41467-021-23242-5
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919