Dong Jun Park1,2,3, Jeong-Eun Park4,5, Tae Hoon Kong4,5, Young Joon Seo6,7,8. 1. Department of Otorhinolaryngology, Yonsei University Wonju College of Medicine, 20 Ilsan- ro, Wonju, Gangwon-do, 26426, South Korea. papapdj@gmail.com. 2. Research Institute of Hearing Enhancement, Wonju, Gangwon-do, 26426, South Korea. papapdj@gmail.com. 3. Department of Surgery, University of California San Diego, 212 Dickinson Street, MC 8236, San Diego, CA, 92103, USA. papapdj@gmail.com. 4. Department of Otorhinolaryngology, Yonsei University Wonju College of Medicine, 20 Ilsan- ro, Wonju, Gangwon-do, 26426, South Korea. 5. Research Institute of Hearing Enhancement, Wonju, Gangwon-do, 26426, South Korea. 6. Department of Otorhinolaryngology, Yonsei University Wonju College of Medicine, 20 Ilsan- ro, Wonju, Gangwon-do, 26426, South Korea. okas2000@hanmail.net. 7. Research Institute of Hearing Enhancement, Wonju, Gangwon-do, 26426, South Korea. okas2000@hanmail.net. 8. School of Pharmacy and Biomedical Sciences, Curtin University, Bentley, WA, 6102, Australia. okas2000@hanmail.net.
Abstract
BACKGROUND: The application of extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) requires customized materials to target disease or cell damage. We hypothesized that EVs exert different inflammatory effects on one recipient cell, although stem cells of different origins in humans have similar payloads. RESULTS: Here, the payload of EVs released by crosstalk between MSCs and human middle ear epithelial cells (HMEECs) extracted from adipose tissue, bone marrow and tonsils significantly increased the level of anti-inflammatory factors. EVs derived from the co-culture medium decreased TNF-α, COX-2, IL-1β, and IL-6 levels to approximately zero within 3 h in HMEECs. Expression of miR-638 and amyloid-β A4 precursor protein-binding family A member 2 was analyzed using microarrays and gene ontology analysis, respectively. CONCLUSIONS: In conclusion, stem cells of different origins have different payloads through crosstalk with recipient-specific cells. Inducing specific factors in EVs by co-culture with MSCs could be valuable in regenerative medicine.
BACKGROUND: The application of extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) requires customized materials to target disease or cell damage. We hypothesized that EVs exert different inflammatory effects on one recipient cell, although stem cells of different origins in humans have similar payloads. RESULTS: Here, the payload of EVs released by crosstalk between MSCs and human middle ear epithelial cells (HMEECs) extracted from adipose tissue, bone marrow and tonsils significantly increased the level of anti-inflammatory factors. EVs derived from the co-culture medium decreased TNF-α, COX-2, IL-1β, and IL-6 levels to approximately zero within 3 h in HMEECs. Expression of miR-638 and amyloid-β A4 precursor protein-binding family A member 2 was analyzed using microarrays and gene ontology analysis, respectively. CONCLUSIONS: In conclusion, stem cells of different origins have different payloads through crosstalk with recipient-specific cells. Inducing specific factors in EVs by co-culture with MSCs could be valuable in regenerative medicine.
Entities:
Keywords:
Amyloid-β A4 precursor protein-binding family A member 2; Antiinflammation; Extracellular vesicles; Mesenchymal stem cell; miR-638
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