Frederico C Martinho1, Fabio R M Leite2, Rodrigo Arruda-Vasconcelos3, Lidiane M Louzada3, Richard P Darveau4, Brenda P F A Gomes5. 1. Department of Restorative Dentistry, Division of Endodontics, Piracicaba Dental School, State University of Campinas - UNICAMP, Piracicaba, SP, Brazil; Department of Department of Advanced Oral Sciences and Therapeutics, University of Maryland School of Dentistry, Baltimore, MD, USA. Electronic address: fmartinho@umaryland.edu. 2. Department of Dentistry and Oral Health - Section for Periodontology, Aarhus University, Aarhus, Denmark. 3. Department of Restorative Dentistry, Division of Endodontics, Piracicaba Dental School, State University of Campinas - UNICAMP, Piracicaba, SP, Brazil. 4. Department of Periodontics and Oral Biology, University of Washington, Seattle, WA, United States. 5. Department of Restorative Dentistry, Division of Endodontics, Piracicaba Dental School, State University of Campinas - UNICAMP, Piracicaba, SP, Brazil. Electronic address: bpgomes@fop.unicamp.br.
Abstract
INTRODUCTION: We verified the association between selected bacterial profiles and levels of cytokines, chemokines and the expression of signs and symptoms of primary endodontic infection with apical periodontitis (PEIAP). METHODS: Samples were collected from 21 root canals, and macrophages stimulated for 24 h. TNF-α, IL-6, IL-10, IL-12p70, INF-γ, and CCL2 were measured using Cytometric Bead Array. We investigated the overlapping networks between cytokines and chemokine with regression analysis. Checkerboard DNA-DNA hybridization was used to assess 40 target bacteria species. Using factor analysis, bacterial species aggregated in two factors. The association of bacteria species-based factors on cytokine and chemokine levels and clinical features was estimated with regression analysis. RESULTS: A negative relationship between IL-10 (anti-inflammatory cytokine) and CCL2, TNF-α, as well as INF-γ (pro-inflammatory cytokines) (all, P<.05) was observed. CCL2 was positively correlated with TNF-α (p<.01). Thirty-eight bacteria species were detected in PEIAP. The first bacteria species-based factor was associated with the size of radiolucent area (coef. 15.42) and tenderness to percussion/pain on palpation (coef. 20.79). The second factor was associated with CCL2 levels (coef. 1.28). CONCLUSION: Different bacterial profiles can be differentially related to the expression of inflammatory proteins and the experience of clinical features.
INTRODUCTION: We verified the association between selected bacterial profiles and levels of cytokines, chemokines and the expression of signs and symptoms of primary endodontic infection with apical periodontitis (PEIAP). METHODS: Samples were collected from 21 root canals, and macrophages stimulated for 24 h. TNF-α, IL-6, IL-10, IL-12p70, INF-γ, and CCL2 were measured using Cytometric Bead Array. We investigated the overlapping networks between cytokines and chemokine with regression analysis. Checkerboard DNA-DNA hybridization was used to assess 40 target bacteria species. Using factor analysis, bacterial species aggregated in two factors. The association of bacteria species-based factors on cytokine and chemokine levels and clinical features was estimated with regression analysis. RESULTS: A negative relationship between IL-10 (anti-inflammatory cytokine) and CCL2, TNF-α, as well as INF-γ (pro-inflammatory cytokines) (all, P<.05) was observed. CCL2 was positively correlated with TNF-α (p<.01). Thirty-eight bacteria species were detected in PEIAP. The first bacteria species-based factor was associated with the size of radiolucent area (coef. 15.42) and tenderness to percussion/pain on palpation (coef. 20.79). The second factor was associated with CCL2 levels (coef. 1.28). CONCLUSION: Different bacterial profiles can be differentially related to the expression of inflammatory proteins and the experience of clinical features.
Authors: Frederico C Martinho; Bruna J M Corazza; Rayana D Khoury; Esteban I F Orozco; Cassia C Toia; Felipe P Machado; Marcia C Valera Journal: Clin Oral Investig Date: 2022-06-20 Impact factor: 3.573
Authors: Johnathan P Velardi; Theeb A Alquria; Rayyan A Alfirdous; Bruna J M Corazza; Ana P M Gomes; Eduardo G Silva; Ina L Griffin; Patricia A Tordik; Frederico C Martinho Journal: Sci Rep Date: 2022-03-22 Impact factor: 4.379