Literature DB >> 3400989

Analysis of proteinuria using a commercial system for automated electrophoresis and isoelectric focusing.

P J Jackson1, C J Sampson, E H Cooper, D Heney, J T Brocklebank.   

Abstract

We describe an investigation of proteinuria using Pharmacia PhastSystemTM electrophoresis apparatus. The analysis of urinary proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of unconcentrated urine followed by silver staining took about 2 h and could clearly demonstrate tubular dysfunction or glomerular damage in urines with a negative or only trace-positive dip-stick test for protein. In addition, we show the identification of urinary proteins by immunoblotting from SDS-PAGE gels and the characterisation of Bence-Jones proteins by isoelectric focusing (IEF) and immunoblotting.

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Year:  1988        PMID： 3400989     DOI： 10.1177/000456328802500322

Source DB:  PubMed          Journal:  Ann Clin Biochem        ISSN： 0004-5632            Impact factor:   2.057


  6 in total

1.  Electrophoretic analysis of urinary proteins in diabetic adolescents.

Authors:  G Koliakos; F Papachristou; M Papadopoulou; V Trachana; M Gaitatzi; I Sotiriou
Journal:  J Clin Lab Anal       Date:  2001       Impact factor: 2.352

2.  Urinary protein analysis in pre- and postoperative cancer patients.

Authors:  Kayo Yokomizo; Shiro Iijima; Nobue Sakai; Yuriko Kurihara; Nobuo Hitratsuka; Kagami Nagai; Takehisa Iwai; Kiyoko Shiba
Journal:  J Clin Lab Anal       Date:  2005       Impact factor: 2.352

3.  Progressive renal toxicity due to ifosfamide.

Authors:  D Heney; J Wheeldon; P Rushworth; C Chapman; I J Lewis; C C Bailey
Journal:  Arch Dis Child       Date:  1991-08       Impact factor: 3.791

4.  Electrophoretic study of the physico-chemical characteristics of Bence-Jones proteinuria and its association with kidney damage.

Authors:  M C Diemert; L Musset; O Gaillard; S Escolano; A Baumelou; F Rousselet; J Galli
Journal:  J Clin Pathol       Date:  1994-12       Impact factor: 3.411

5.  Sodium dodecyl sulphate polyacrylamide gel electrophoresis of urinary proteins in steroid-responsive and steroid-resistant nephrotic syndrome in children.

Authors:  G Ramjee; H M Coovadia; M Adhikari
Journal:  Pediatr Nephrol       Date:  1994-12       Impact factor: 3.714

6.  Sodium dodecyl sulphate polyacrylamide gel electrophoresis patterns of proteinuria in various renal diseases of childhood.

Authors:  T Brocklebank; E H Cooper; K Richmond
Journal:  Pediatr Nephrol       Date:  1991-07       Impact factor: 3.714
  6 in total

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