Literature DB >> 34009588

RNAi-Based Gene Silencing in Sugarcane for Production of Biofuel.

Naveenarani Murugan1, Chakravarthi Mohan2,3, Baskaran Kannan4.   

Abstract

RNA interference (RNAi) is an innate cellular mechanism triggered by a double-stranded RNA (dsRNA) molecule causing selective inhibition of gene expression. Here, we demonstrated the RNAi technology for gene silencing in sugarcane for biofuel production. This chapter describes an efficient model system that established to target the caffeic acid O-methyltransferase (COMT) gene and the RNAi construct is designed and delivered through Agrobacterium mediated stable sugarcane transformation. Also, the approach for an analysis of resulting putative transgenic plants for a targeted RNAi mediated gene silencing is described.

Entities:  

Keywords:  Agrobacterium; Biofuel; Caffeic acid O-methyltransferase (COMT); Gene silencing; RNA interference; Sugarcane; Transgenic sugarcane

Year:  2021        PMID： 34009588     DOI： 10.1007/978-1-0716-1323-8_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

1.  Rapid isolation of high molecular weight plant DNA.

Authors:  M G Murray; W F Thompson
Journal:  Nucleic Acids Res       Date:  1980-10-10       Impact factor: 16.971

2.  RNAi suppression of lignin biosynthesis in sugarcane reduces recalcitrance for biofuel production from lignocellulosic biomass.

Authors:  Je Hyeong Jung; Walid M Fouad; Wilfred Vermerris; Maria Gallo; Fredy Altpeter
Journal:  Plant Biotechnol J       Date:  2012-08-24       Impact factor: 9.803

3.  Precision breeding for RNAi suppression of a major 4-coumarate:coenzyme A ligase gene improves cell wall saccharification from field grown sugarcane.

Authors:  Je Hyeong Jung; Baskaran Kannan; Hugo Dermawan; Geoffrey W Moxley; Fredy Altpeter
Journal:  Plant Mol Biol       Date:  2016-08-22       Impact factor: 4.076

4.  RNA interference suppression of lignin biosynthesis increases fermentable sugar yields for biofuel production from field-grown sugarcane.

Authors:  Je Hyeong Jung; Wilfred Vermerris; Maria Gallo; Jeffrey R Fedenko; John E Erickson; Fredy Altpeter
Journal:  Plant Biotechnol J       Date:  2013-04-02       Impact factor: 9.803

5.  Molecular mechanisms of RNA-triggered gene silencing machineries.

Authors:  Zhonghan Li; Tariq M Rana
Journal:  Acc Chem Res       Date:  2012-02-03       Impact factor: 22.384

Review 6.  Small RNAs in plants: recent development and application for crop improvement.

Authors:  Ayushi Kamthan; Abira Chaudhuri; Mohan Kamthan; Asis Datta
Journal:  Front Plant Sci       Date:  2015-04-02       Impact factor: 5.753

7.  TALEN mediated targeted mutagenesis of the caffeic acid O-methyltransferase in highly polyploid sugarcane improves cell wall composition for production of bioethanol.

Authors:  Je Hyeong Jung; Fredy Altpeter
Journal:  Plant Mol Biol       Date:  2016-06-15       Impact factor: 4.076

8.  RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production.

Authors:  William P Bewg; Charleson Poovaiah; Wu Lan; John Ralph; Heather D Coleman
Journal:  Biotechnol Biofuels       Date:  2016-12-20       Impact factor: 6.040

Review 9.  Potentials, Challenges, and Genetic and Genomic Resources for Sugarcane Biomass Improvement.

Authors:  Ramkrishna Kandel; Xiping Yang; Jian Song; Jianping Wang
Journal:  Front Plant Sci       Date:  2018-02-16       Impact factor: 5.753

10.  Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility.

Authors:  Wagner Rodrigo de Souza; Thályta Fraga Pacheco; Karoline Estefani Duarte; Bruno Leite Sampaio; Patrícia Abrão de Oliveira Molinari; Polyana Kelly Martins; Thaís Ribeiro Santiago; Eduardo Fernandes Formighieri; Felipe Vinecky; Ana Paula Ribeiro; Bárbara Andrade Dias Brito da Cunha; Adilson Kenji Kobayashi; Rowan Andrew Craig Mitchell; Dasciana de Sousa Rodrigues Gambetta; Hugo Bruno Correa Molinari
Journal:  Biotechnol Biofuels       Date:  2019-05-06       Impact factor: 6.040
  10 in total

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