Cheng-Lin Xu1, Wei-Qun Guan2, Xue-Ying Wang3. 1. Department of Stomatology, Affiliated Hospital of Putian University, Putian, 351100, China. 2. Department of Stomatology, Union Hospital, Fujian Medical University, No. 29 of Xinquan Street, Gulou District, Fuzhou, 350001, China. weiqunguandr_12@163.com. 3. Department of Stomatology, Union Hospital, Fujian Medical University, No. 29 of Xinquan Street, Gulou District, Fuzhou, 350001, China.
Abstract
BACKGROUND: This study aimed to investigate the expression level of the GATA6 gene in different oral cancer cells. METHODS: In this study, we sub-cultured normal oral epithelial cell lines HOK, human tongue squamous cell carcinoma cell lines CAL-27 and SCC-4, and human salivary gland adenoid cystic carcinoma cell lines SACC-LM and SACC-83. Subsequently, we used reverse transcription-polymerase chain reaction RT-PCR and Western blot methods to detect the mRNA and the protein expressions of GATA6 in normal oral epithelial cells, human tongue squamous cell carcinoma cells, and human salivary gland adenoid cystic carcinoma cells. RESULTS: The results of this study showed that the mRNA expression levels of GATA6 in CAL-27, SCC-4, and SACC-LM cells were significantly increased when compared with the HOK cells. However, the mRNA expression level of GATA6 in the SACC-83 cells had no significant difference compared with the HOK cells. The protein expression levels of GATA6 in the SCC-4 and SACC-LM cells were, however, significantly increased whereas the protein expression levels of GATA6 in the CAL-27 and SACC-83 cells had no significant difference when compared with the HOK cells. CONCLUSION: The GATA6 gene may be related to the occurrence and progression of certain oral cancers.
BACKGROUND: This study aimed to investigate the expression level of the GATA6 gene in different oral cancer cells. METHODS: In this study, we sub-cultured normal oral epithelial cell lines HOK, humantongue squamous cell carcinoma cell lines CAL-27 and SCC-4, and humansalivary gland adenoid cystic carcinoma cell lines SACC-LM and SACC-83. Subsequently, we used reverse transcription-polymerase chain reaction RT-PCR and Western blot methods to detect the mRNA and the protein expressions of GATA6 in normal oral epithelial cells, humantongue squamous cell carcinoma cells, and humansalivary gland adenoid cystic carcinoma cells. RESULTS: The results of this study showed that the mRNA expression levels of GATA6 in CAL-27, SCC-4, and SACC-LM cells were significantly increased when compared with the HOK cells. However, the mRNA expression level of GATA6 in the SACC-83 cells had no significant difference compared with the HOK cells. The protein expression levels of GATA6 in the SCC-4 and SACC-LM cells were, however, significantly increased whereas the protein expression levels of GATA6 in the CAL-27 and SACC-83 cells had no significant difference when compared with the HOK cells. CONCLUSION: The GATA6 gene may be related to the occurrence and progression of certain oral cancers.
Entities:
Keywords:
GATA6; Oral cancer; Oral epithelial cell; RT-PCR; Western blot
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