Di Feng1, Baolin Chen1, Benhua Zeng2, Lu Xiao1, Junyan Yan1, Ting Yang1, Jiang Zhu1, Tingyu Li1, Lan Wang3, Hong Wei2, Jie Chen4. 1. Children's Nutrition Research Center, Children's Hospital of Chongqing Medical University; Chongqing Key Laboratory of Childhood Nutrition and Health; Ministry of Education Key Laboratory of Child Development and disorders; National Clinical Research Center for Child Health and Disorders, Chongqing, China. 2. Laboratory Animal Department, College of Basic Medicine, Army Medical University, Chongqing, China. 3. Shanxi Provincial Corps Hospital of Chinese People's Armed Police Force, Taiyuan, China. 4. Children's Nutrition Research Center, Children's Hospital of Chongqing Medical University; Chongqing Key Laboratory of Childhood Nutrition and Health; Ministry of Education Key Laboratory of Child Development and disorders; National Clinical Research Center for Child Health and Disorders, Chongqing, China. Electronic address: jchen010@hospital.cqmu.edu.cn.
Abstract
OBJECTIVE: This study explores the effects of fecal microbiota from children with vitamin A (VA) deficiency on colonic mucosal barrier function. METHODS: The composition of gut microbes was identified in children with different VA levels, then feces from children with normal VA or VA deficiency was collected separately and transplanted into germ-free (GF) mice, respectively. Three weeks after transplantation, the colon morphology, colonic tight junction proteins, gut microbes, and metabolites were evaluated. RESULTS: In children, Bifidobacterium and Bacteroides were positively correlated with VA levels. Colonization of VA deficiency fecal microbiota markedly impaired colonic development in GF mice, down-regulated colonic tight junction-related proteins occludin and claudin-1, and reduced immunoglobulin A secretion. Furthermore, fecal microbiota transplantation with different VA levels altered composition of gut microbes and bile acid metabolism pathways in GF mice. CONCLUSION: These data suggest that fecal microbiota from children with VA deficiency attenuates colonic barrier function in GF mice, which may be achieved by changing the bile acid metabolic pathways.
OBJECTIVE: This study explores the effects of fecal microbiota from children with vitamin A (VA) deficiency on colonic mucosal barrier function. METHODS: The composition of gut microbes was identified in children with different VA levels, then feces from children with normal VA or VA deficiency was collected separately and transplanted into germ-free (GF) mice, respectively. Three weeks after transplantation, the colon morphology, colonic tight junction proteins, gut microbes, and metabolites were evaluated. RESULTS: In children, Bifidobacterium and Bacteroides were positively correlated with VA levels. Colonization of VA deficiency fecal microbiota markedly impaired colonic development in GF mice, down-regulated colonic tight junction-related proteins occludin and claudin-1, and reduced immunoglobulin A secretion. Furthermore, fecal microbiota transplantation with different VA levels altered composition of gut microbes and bile acid metabolism pathways in GF mice. CONCLUSION: These data suggest that fecal microbiota from children with VA deficiency attenuates colonic barrier function in GF mice, which may be achieved by changing the bile acid metabolic pathways.
Authors: Katherine M DiGuilio; Elizabeth Rybakovsky; Reza Abdavies; Romy Chamoun; Colleen A Flounders; Ariel Shepley-McTaggart; Ronald N Harty; James M Mullin Journal: Int J Mol Sci Date: 2022-03-10 Impact factor: 5.923