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Literature DB >> 3398839

High malic enzyme activity in tumor cells and its cross-reaction with anti-pigeon liver malic enzyme serum.

P L Kam¹, C C Lin, J C Li, C L Meng, G G Chang.

Abstract

Rabbit antibodies against pigeon liver malic enzyme (EC 1.1.1.40) were prepared. The antiserum gave single precipitation line with crude pigeon liver extract. Cross reaction was observed with partially purified malic enzyme or crude extract from chicken liver. Positive cross reaction was also observed with the concentrated cytosolic fraction of two human carcinoma cell lines which were demonstrated to contain high malic enzyme activity. All other proteins examined did not react with the antibodies. When purified pigeon liver malic enzyme was mixed with the antiserum in vitro, a time-dependent inactivation of the enzyme activity was observed. Protection of the enzyme activity against antiserum inactivation was afforded by NADP+ or L-malate. Metal Mn2+ gave little protection.

Entities: Chemical Disease Species

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Year: 1988 PMID： 3398839 DOI： 10.1007/bf02424560

Source DB: PubMed Journal: Mol Cell Biochem ISSN： 0300-8177 Impact factor: 3.396

35 in total

1. Reversible oxidative decarboxylation of malic acid.

Authors: S OCHOA; A MEHLER; A KORNBERG
Journal: J Biol Chem Date: 1947-03 Impact factor: 5.157

2. Human malic enzyme: high-frequency polymorphism of the mitochondrial form.

Authors: P T Cohen; G S Omenn
Journal: Biochem Genet Date: 1972-12 Impact factor: 1.890

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

4. A simplified method for culture of oral epithelial cells.

Authors: G S Schuster; B B Singh; D A Welter; J F Erbland
Journal: J Oral Pathol Date: 1985-04

5. Evidence that glutamine, not sugar, is the major energy source for cultured HeLa cells.

Authors: L J Reitzer; B M Wice; D Kennell
Journal: J Biol Chem Date: 1979-04-25 Impact factor: 5.157

6. Sub-unit structure of soluble and mitochondrial malic enzyme: demonstration of human mitochondrial enzyme in human-mouse hybrids.

Authors: S Povey; D E Wilson; H Harris; I P Gormley; P Perry; K E Buckton
Journal: Ann Hum Genet Date: 1975-10 Impact factor: 1.670

7. The pathways of glutamate and glutamine oxidation by tumor cell mitochondria. Role of mitochondrial NAD(P)+-dependent malic enzyme.

Authors: R W Moreadith; A L Lehninger
Journal: J Biol Chem Date: 1984-05-25 Impact factor: 5.157

4. Repressing malic enzyme 1 redirects glucose metabolism, unbalances the redox state, and attenuates migratory and invasive abilities in nasopharyngeal carcinoma cell lines.

Authors: Fang-Jing Zheng; Hao-Bin Ye; Man-Si Wu; Yi-Fan Lian; Chao-Nan Qian; Yi-Xin Zeng
Journal: Chin J Cancer Date: 2012-11-01

4 in total

High malic enzyme activity in tumor cells and its cross-reaction with anti-pigeon liver malic enzyme serum.

1. Reversible oxidative decarboxylation of malic acid.

2. Human malic enzyme: high-frequency polymorphism of the mitochondrial form.

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

4. A simplified method for culture of oral epithelial cells.

5. Evidence that glutamine, not sugar, is the major energy source for cultured HeLa cells.

6. Sub-unit structure of soluble and mitochondrial malic enzyme: demonstration of human mitochondrial enzyme in human-mouse hybrids.

7. The pathways of glutamate and glutamine oxidation by tumor cell mitochondria. Role of mitochondrial NAD(P)+-dependent malic enzyme.

8. Rapid purification and radioimmunoassay of cytosolic malic enzyme.

9. Isolation of mitochondria from ascites tumor cells permeabilized with digitonin.

10. Mechanism of pigeon liver malic enzyme: modification of essential carboxyl groups.

1. Nonidentity of the cDNA sequence of human breast cancer cell malic enzyme to that from the normal human cell.

2. Proposed cycles for functional glutamate trafficking in synaptic neurotransmission.

3. Biophysical characterization of the dimer and tetramer interface interactions of the human cytosolic malic enzyme.

4. Repressing malic enzyme 1 redirects glucose metabolism, unbalances the redox state, and attenuates migratory and invasive abilities in nasopharyngeal carcinoma cell lines.