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Literature DB >> 339867

Cellular content of the Krebs cycle keto acids in yeasts grown on different nitrogen sources, including hydroxylamine.

Abstract

The cellular pool of Krebs cycle keto acids was followed as a function of growth in three yeasts. The keto acids were analyzed as silylated methoximes by quantitative gas chromatography with capillary glass columns. The 2-oxoglutaric acid content was strikingly high in the hydroxylamine (HA)-tolerant, HA-utilizing Endomycopsis lipolytica when compared to that in the nitrate-utilizing yeast Cryptococcus albidus and Saccharomyces cerevisiae, requiring fully reduced nitrogen for growth. The content of E. lipolytica increased throughout the log phase to maxima of about 200-250 microgram per g dry weight in HA and ammonia media. These amounts are 20-25 times greater than those attained in the two other yeasts. The cellular content of pyruvic acid was at a maximum early in the log phase, amounting to 50-70 microgram per g dry weight for all yeasts. The oxalacetic acid content never exceeded 9 microgram per g dry weight in any of the yeasts. Oximeformation, for which keto acid production is a prerequisite, is discussed as part of the HA-tolerance.

Entities: Chemical Species

Mesh：

Substances：

Year: 1977 PMID： 339867 DOI： 10.1007/bf00406365

Source DB: PubMed Journal: Arch Microbiol ISSN： 0302-8933 Impact factor: 2.552

14 in total

1. A KINETIC STUDY OF THE ASSIMILATION OF (15N)-AMMONIA AND THE SYNTHESIS OF AMINO ACIDS IN AN EXPONENTIALLY GROWING CULTURE OF CANDIDA UTILIS.

Authors: A P SIMS; B F FOLKES
Journal: Proc R Soc Lond B Biol Sci Date: 1964-02-18

8. The effect of culture age, chloramphenicol and B6 inhibitors on intra- and extracellular keto and amino acids of Escherichia coli B.

Authors: R P Raunio; M Leppävirta
Journal: J Gen Microbiol Date: 1975-03

9. Oximinoacids in the inorganic nitrogen metabolism.

Authors: I Anderson; B Norkrans; G Odham
Journal: Experientia Date: 1972-10-15

10. [Assimilation of nitrites and organic nitrogen compounds by various yeasts and yeast-like organisms].

Authors: F Abadie
Journal: Ann Inst Pasteur (Paris) Date: 1968-08

1 in total

1. Changes in free amino acid content and activities of amination and transamination enzymes in yeasts grown on different inorganic nitrogen sources, including hydroxylamine.

Authors: B Norkrans; I Tunblad-Johansson
Journal: Antonie Van Leeuwenhoek Date: 1981 Impact factor: 2.271

1 in total

Cellular content of the Krebs cycle keto acids in yeasts grown on different nitrogen sources, including hydroxylamine.

1. A KINETIC STUDY OF THE ASSIMILATION OF (15N)-AMMONIA AND THE SYNTHESIS OF AMINO ACIDS IN AN EXPONENTIALLY GROWING CULTURE OF CANDIDA UTILIS.

2. The free amino acids in growing and non-growing populations of Escherichia coli.

3. Gas chromatographic separation of Krebs-cycle metabolites.

4. The enzymic reduction of nitrite to ammonia by reduced pyridine nucleotides.

5. The quantitative recovery and colorimetric estimation of amino-acids separated by paper chromatography.

6. Accumulation of keto acids during the growth cycle of Escherichia coli.

7. Hydroxylamine as the sole nitrogen source for growth of some Candida sp.

8. The effect of culture age, chloramphenicol and B6 inhibitors on intra- and extracellular keto and amino acids of Escherichia coli B.

9. Oximinoacids in the inorganic nitrogen metabolism.

10. [Assimilation of nitrites and organic nitrogen compounds by various yeasts and yeast-like organisms].

1. Changes in free amino acid content and activities of amination and transamination enzymes in yeasts grown on different inorganic nitrogen sources, including hydroxylamine.