Literature DB >> 33986447

BABEL: using deep learning to translate between single-cell datasets.

George Andrew S Inglis1.   

Abstract

Entities:  

Year:  2021        PMID: 33986447      PMCID: PMC8119457          DOI: 10.1038/s42003-021-02135-9

Source DB:  PubMed          Journal:  Commun Biol        ISSN: 2399-3642


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Pixabay Technological advances within the past decade have allowed researchers to generate multiomic profiles within single-cells: gene expression and chromatin accessibility (SNARE-seq, sci-CAR), gene expression and protein epitopes (CITE-seq), or chromatin accessibility and protein epitopes (Pi-ATAC, ASAP-seq). While these methods enable multimodal analyses of gene regulation with single-cell resolution, the protocols are both technically challenging and expensive, potentially restricting their feasibility among researchers. As an alternative approach to these multiomic methods, Wu et al.[1] at Stanford University recently developed BABEL, a deep learning tool that can effectively translate one single-cell modality (chromatin accessibility) into a paired dataset (gene expression) for downstream analysis. The authors first trained BABEL on three cell types that had been separately profiled using single-cell (sc)ATAC-seq (chromatin accessibility) or scRNA-seq. They reported that BABEL was capable of using scATAC-seq data to robustly predict gene expression, maintaining a strong correlation with complementary scRNA-seq datasets and outperforming tools like ArchR or MAESTRO that impute gene activity from chromatin accessibility. Furthermore, BABEL-derived gene expression data preserved similar clustering and cell type-specific expression patterns observed in complementary scRNA-seq datasets. The authors also observed that BABEL could infer gene expression from scATAC-seq data in multiple human samples that were not used when training the algorithm, such as lymphoblastoma cell lines or clinical basal cell carcinoma isolates. Interestingly, BABEL also maintained a high level of accuracy when analyzing cerebral cortex or skin samples taken from adult mice, suggesting that it may be applicable for single-cell analyses across multiple species. While the authors primarily focused on translating human scATAC-seq results into analogous gene expression data, they also demonstrated that BABEL could work in reverse, inferring chromatin accessibility from scRNA-seq data. Similarly, BABEL could translate scATAC-seq input into protein epitope expression, highlighting its flexible framework. Altogether, BABEL offers a robust and versatile approach to translating single-cell datasets between modalities. Given the practical and financial barriers to performing multiple single-cell experiments, BABEL represents a promising tool to make multimodal analyses more accessible to researchers and thereby maximize the potential of their data.
  1 in total

1.  BABEL enables cross-modality translation between multiomic profiles at single-cell resolution.

Authors:  Kevin E Wu; Kathryn E Yost; Howard Y Chang; James Zou
Journal:  Proc Natl Acad Sci U S A       Date:  2021-04-13       Impact factor: 11.205

  1 in total

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