| Literature DB >> 33977475 |
Abstract
Various biochemical methods have been introduced to detect and characterize small GTPases and Ras. Luminescence-based techniques cover most of the currently used methods, utilizing single- or multi-luminophore-conjugated molecules and external probes. Here we describe methods enabling Ras activity and activation state monitoring in vitro. This chapter focuses mainly on luminescence-based techniques.Keywords: Environment-sensitive labels; Fluorescence polarization (FP); Förster resonance energy transfer (FRET); GTP- and GDP-specific antibodies; Quenching resonance energy transfer (QRET); Ras; Ras-binding domain (RBD); Small GTPases; Thermal stability assay (TSA); Time-resolved fluorescence resonance energy transfer (TR-FRET)
Year: 2021 PMID： 33977475 DOI： 10.1007/978-1-0716-1190-6_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745