Screening Fermentation and Extract Generation.

This chapter describes the process of fermenting actinomycetes in flask and the generation of extracts from these broths. A medium for secondary metabolite production and a general procedure for flask fermentation are specified. Directions are given to reproduce aeration when using different flasks. The generation of extracts is based on a solvent mixture that could be varied in order to improve the extraction of products with different polarity. These extracts are then stored in a 96-well microtube format to facilitate their usage for the screening of bioactive compounds.