Nur Balci1, Şivge Kurgan2, Ali Çekici3, Tülin Çakır3, Muhittin A Serdar4. 1. Department of Periodontology, Faculty of Dentistry, Istanbul Medipol University, Istanbul, Turkey. nbalci@medipol.edu.tr. 2. Department of Periodontology, Faculty of Dentistry, Ankara University, Ankara, Turkey. 3. Department of Periodontology, Faculty of Dentistry, Istanbul University, Istanbul, Turkey. 4. Department of Medical Biochemistry, School of Medicine, Acıbadem University, Ankara, Turkey.
Abstract
OBJECTIVES: To identify and compare the free amino acids in the saliva of periodontitis patients and healthy individuals and to assess their levels in different periodontal disease types. MATERIALS AND METHODS: There were three groups: healthy individuals (control (C); n = 20), Stage III Grade B generalized periodontitis (GP-B; n = 20), and Stage III Grade C generalized periodontitis (GP-C; n = 20). Clinical periodontal parameters were measured. Amino acid analysis of the saliva was accomplished by liquid chromatography-mass spectrometry (LC MS/MS), taking the mean concentration. RESULTS: Citrulline and carnosine concentrations were significantly higher in patients with periodontitis than in the control group (p < 0.017). Methionine, glutamic acid, and arginine showed significantly higher concentrations in GP-C, whereas proline and tryptophan showed higher concentrations in the GP-B group (p < 0.017). There was a significant correlation between methionine, citrulline, arginine, and carnosine and clinical periodontal parameters. CONCLUSIONS: Our results demonstrate that periodontal status and disease type can result in variations in salivary amino acid (AA) content in correlation with clinical inflammatory signs. The significant correlation of methionine, citrulline, carnosine, and arginine with clinical parameters, regardless of systemic status, suggests that the levels of different salivary free AAs play roles in periodontitis. CLINICAL RELEVANCE: Salivary free AAs may be suggested as a potential diagnostic compound in patients with periodontitis. CLINICAL TRIAL REGISTRATION: ClinicalTrials.gov NCT04642716.
OBJECTIVES: To identify and compare the free amino acids in the saliva of periodontitispatients and healthy individuals and to assess their levels in different periodontal disease types. MATERIALS AND METHODS: There were three groups: healthy individuals (control (C); n = 20), Stage III Grade B generalized periodontitis (GP-B; n = 20), and Stage III Grade C generalized periodontitis (GP-C; n = 20). Clinical periodontal parameters were measured. Amino acid analysis of the saliva was accomplished by liquid chromatography-mass spectrometry (LC MS/MS), taking the mean concentration. RESULTS:Citrulline and carnosine concentrations were significantly higher in patients with periodontitis than in the control group (p < 0.017). Methionine, glutamic acid, and arginine showed significantly higher concentrations in GP-C, whereas proline and tryptophan showed higher concentrations in the GP-B group (p < 0.017). There was a significant correlation between methionine, citrulline, arginine, and carnosine and clinical periodontal parameters. CONCLUSIONS: Our results demonstrate that periodontal status and disease type can result in variations in salivary amino acid (AA) content in correlation with clinical inflammatory signs. The significant correlation of methionine, citrulline, carnosine, and arginine with clinical parameters, regardless of systemic status, suggests that the levels of different salivary free AAs play roles in periodontitis. CLINICAL RELEVANCE: Salivary free AAs may be suggested as a potential diagnostic compound in patients with periodontitis. CLINICAL TRIAL REGISTRATION: ClinicalTrials.gov NCT04642716.
Authors: Tobias V Lanz; Simon Becker; Soumya R Mohapatra; Christiane A Opitz; Wolfgang Wick; Michael Platten Journal: Amino Acids Date: 2017-04-18 Impact factor: 3.520
Authors: Ş Kurgan; C Önder; N Balcı; Ö Fentoğlu; F Eser; M Balseven; M A Serdar; D N Tatakis; M Günhan Journal: J Periodontal Res Date: 2016-10-26 Impact factor: 4.419
Authors: Nelson A Kretzmann; Henrique Fillmann; José L Mauriz; Claudio Augusto Marroni; Norma Marroni; Javier González-Gallego; María J Tuñón Journal: Inflamm Bowel Dis Date: 2008-11 Impact factor: 5.325