Chromatographic characterization of a silica hydride-based amide stationary phase.

An amide phase based on a porous silica hydride support material is tested for retention characteristics in both the reversed-phase and aqueous normal-phase modes. A series of retention maps (retention time vs. mobile phase composition) were obtained using reference standards of varying analyte sizes, functionalities, and polarities. An assessment of the specific column selectivity is made and classes of compounds are identified which show high potential for effective retention, resolution, and efficiency when using amide functionalized silica hydride columns for reversed-phase and aqueous-normal phase separation. Several practical applications are presented that illustrate the capabilities of this particular column format. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.