Literature DB >> 3397303

Differentiating cardiac elastin, collagen and microfibrils with NaOH at the ultrastructural level.

C P Dickson1, T F Robinson.   

Abstract

NaOH solutions extract elastin and collagen from epoxy-embedded thin sections containing rat cardiac connective tissue. Extraction results in a reverse staining effect of elastin and collagen ultrastructure. Microfibril contrast is enhanced by NaOH treatment. This phenomenon finds application in the possibility of differentiating elastin, collagen, and microfibrils at the ultrastructural level.

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Year:  1988        PMID： 3397303     DOI： 10.1007/bf00489912

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  18 in total

1.  HISTOCHEMICAL SPECIFITY OF STAINING METHODS FOR CONNECTIVE TISSUE FIBERS: RESORCIN-FUCHSIN AND VAN GIESON'S PICRO-FUCHSIN.

Authors:  H PUCHTLER; F SWEAT
Journal:  Z Zellforch Microsk Anat Histochem       Date:  1964

2.  Microfibrils: fine filamentous components of the tissue space.

Authors:  F N LOW
Journal:  Anat Rec       Date:  1962-02

3.  Orcein, collastin and pseudo-elastica: a re-investigation of Unna's concepts.

Authors:  H Puchtler; S N Meloan
Journal:  Histochemistry       Date:  1979-11

4.  A low-viscosity epoxy resin embedding medium for electron microscopy.

Authors:  A R Spurr
Journal:  J Ultrastruct Res       Date:  1969-01

5.  A routine method for contrasting elastin at the ultrastructural level.

Authors:  S Franc; R Garrone; A Bosch; J M Franc
Journal:  J Histochem Cytochem       Date:  1984-02       Impact factor: 2.479

6.  The elastic system fibers.

Authors:  G Cotta-Pereira; F G Rodrigo; J F David-Ferreira
Journal:  Adv Exp Med Biol       Date:  1977       Impact factor: 2.622

7.  Developing elastic tissue. An electron microscopic study.

Authors:  E N Albert
Journal:  Am J Pathol       Date:  1972-10       Impact factor: 4.307

8.  Aldehyde-fuchsin: historical and chemical considerations.

Authors:  H Puchtler; S N Meloan; F S Waldrop
Journal:  Histochemistry       Date:  1979-04-03

9.  Simple methods for "staining with lead" at high pH in electron microscopy.

Authors:  M J KARNOVSKY
Journal:  J Biophys Biochem Cytol       Date:  1961-12

10.  The fine structure of elastic fibers.

Authors:  T K Greenlee; R Ross; J L Hartman
Journal:  J Cell Biol       Date:  1966-07       Impact factor: 10.539
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  2 in total

1.  Organization of rat mesenteric artery after removal of cells of extracellular matrix components.

Authors:  H M Walker-Caprioglio; J A Trotter; J Mercure; S A Little; L J McGuffee
Journal:  Cell Tissue Res       Date:  1991-04       Impact factor: 5.249

2.  Establishment and characterization of two new human ovarian cancer cell lines UWOV1 and UWOV2 and a subline UWOV2 (Sf) growing in serum-free conditions: growth characteristics, biochemical, and cytogenetic studies.

Authors:  T Golombick; R Dansey; W R Bezwoda; J Rosendorff
Journal:  In Vitro Cell Dev Biol       Date:  1990-05
  2 in total

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