Noriyoshi Isozumi1, Yuya Masubuchi1, Tomohiro Imamura2, Masashi Mori2, Hironori Koga2, Shinya Ohki3. 1. Center for Nano Materials and Technology (CNMT), Japan Advanced Institute of Science and Technology (JAIST), 1-1 Asahidai, Nomi, Ishikawa, 923-1292, Japan. 2. Ishikawa Prefectural University, 1-308, Suematsu, Nonoichi, Ishikawa, 921-8836, Japan. 3. Center for Nano Materials and Technology (CNMT), Japan Advanced Institute of Science and Technology (JAIST), 1-1 Asahidai, Nomi, Ishikawa, 923-1292, Japan. shinya-o@jaist.ac.jp.
Abstract
A model legume, Medicago truncatula, has over 600 nodule-specific cysteine-rich (NCR) peptides required for symbiosis with rhizobia. Among them, NCR169, an essential factor for establishing symbiosis, has four cysteine residues that are indispensable for its function. However, knowledge of NCR169 structure and mechanism of action is still lacking. In this study, we solved two NMR structures of NCR169 caused by different disulfide linkage patterns. We show that both structures have a consensus C-terminal β-sheet attached to an extended N-terminal region with dissimilar features; one moves widely, whereas the other is relatively stapled. We further revealed that the disulfide bonds of NCR169 contribute to its structural stability and solubility. Regarding the function, one of the NCR169 oxidized forms could bind to negatively charged bacterial phospholipids. Furthermore, the positively charged lysine-rich region of NCR169 may be responsible for its antimicrobial activity against Escherichia coli and Sinorhizobium meliloti. This active region was disordered even in the phospholipid bound state, suggesting that the disordered conformation of this region is key to its function. Morphological observations suggested the mechanism of action of NCR169 on bacteria. The present study on NCR169 provides new insights into the structure and function of NCR peptides.
A model legume, Medicago truncatula, has over 600 nodule-specific n class="Chemical">cysteine-rich (NCR) peptides required for symbiosis with rhizobia. Among them, NCR169, an essential factor for establishing symbiosis, has four cysteine residues that are indispensable for its function. However, knowledge of NCR169 structure and mechanism of action is still lacking. In this study, we solved two NMR structures of NCR169 caused by different disulfide linkage patterns. We show that both structures have a consensus C-terminal β-sheet attached to an extended N-terminal region with dissimilar features; one moves widely, whereas the other is relatively stapled. We further revealed that the disulfide bonds of NCR169 contribute to its structural stability and solubility. Regarding the function, one of the NCR169 oxidized forms could bind to negatively charged bacterial phospholipids. Furthermore, the positively charged lysine-rich region of NCR169 may be responsible for its antimicrobial activity against Escherichia coli and Sinorhizobium meliloti. This active region was disordered even in the phospholipid bound state, suggesting that the disordered conformation of this region is key to its function. Morphological observations suggested the mechanism of action of NCR169 on bacteria. The present study on NCR169 provides new insights into the structure and function of NCR peptides.
Authors: Willem Van de Velde; Grigor Zehirov; Agnes Szatmari; Monika Debreczeny; Hironobu Ishihara; Zoltan Kevei; Attila Farkas; Kata Mikulass; Andrea Nagy; Hilda Tiricz; Beatrice Satiat-Jeunemaître; Benoit Alunni; Mickael Bourge; Ken-ichi Kucho; Mikiko Abe; Attila Kereszt; Gergely Maroti; Toshiki Uchiumi; Eva Kondorosi; Peter Mergaert Journal: Science Date: 2010-02-26 Impact factor: 47.728
Authors: Harald Marx; Catherine E Minogue; Dhileepkumar Jayaraman; Alicia L Richards; Nicholas W Kwiecien; Alireza F Siahpirani; Shanmugam Rajasekar; Junko Maeda; Kevin Garcia; Angel R Del Valle-Echevarria; Jeremy D Volkening; Michael S Westphall; Sushmita Roy; Michael R Sussman; Jean-Michel Ané; Joshua J Coon Journal: Nat Biotechnol Date: 2016-10-17 Impact factor: 54.908
Authors: Jon Penterman; Ryan P Abo; Nicole J De Nisco; Markus F F Arnold; Renato Longhi; Matteo Zanda; Graham C Walker Journal: Proc Natl Acad Sci U S A Date: 2014-02-05 Impact factor: 11.205
Authors: Jesús Montiel; J Allan Downie; Attila Farkas; Péter Bihari; Róbert Herczeg; Balázs Bálint; Peter Mergaert; Attila Kereszt; Éva Kondorosi Journal: Proc Natl Acad Sci U S A Date: 2017-04-24 Impact factor: 11.205
Authors: Beatrix Horváth; Ágota Domonkos; Attila Kereszt; Attila Szűcs; Edit Ábrahám; Ferhan Ayaydin; Károly Bóka; Yuhui Chen; Rujin Chen; Jeremy D Murray; Michael K Udvardi; Éva Kondorosi; Péter Kaló Journal: Proc Natl Acad Sci U S A Date: 2015-09-23 Impact factor: 11.205
Authors: Kathryn M Jones; Hajime Kobayashi; Bryan W Davies; Michiko E Taga; Graham C Walker Journal: Nat Rev Microbiol Date: 2007-08 Impact factor: 60.633
Authors: Rui M Lima; Balaji Baburao Rathod; Hilda Tiricz; Dian H O Howan; Mohamad Anas Al Bouni; Sándor Jenei; Edit Tímár; Gabriella Endre; Gábor K Tóth; Éva Kondorosi Journal: Front Mol Biosci Date: 2022-06-09
Authors: Marina P Slezina; Ekaterina A Istomina; Ekaterina V Kulakovskaya; Tatyana V Korostyleva; Tatyana I Odintsova Journal: Int J Mol Sci Date: 2022-07-29 Impact factor: 6.208
Authors: Evgeny A Zorin; Marina S Kliukova; Alexey M Afonin; Emma S Gribchenko; Mikhail L Gordon; Anton S Sulima; Aleksandr I Zhernakov; Olga A Kulaeva; Daria A Romanyuk; Pyotr G Kusakin; Anna V Tsyganova; Viktor E Tsyganov; Igor A Tikhonovich; Vladimir A Zhukov Journal: Front Plant Sci Date: 2022-09-14 Impact factor: 6.627