Payam Siyadat1, Hossein Ayatollahi2, Mahmood Barati3, Maryam Sheikhi2, Minoo Shahidi1. 1. Department of Hematology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran. 2. Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. 3. Department of Biotechnology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran.
Abstract
BACKGROUND: Acute lymphoblastic leukemia (ALL) is a highly heterogeneous malignancy that accounts for nearly 75% of leukemias in children. While the exact mechanism of ALL is not fully understood, some genetic variants have been implicated as associated with ALL susceptibility. The association between some genetic variants in miRNA genes and ALL risk has been described previously. A previous study suggested that mir-612 rs12803915 G> A may be associated with pediatric ALL risk. High-resolution melting (HRM) analysis is a reliable method that can be applied for polymorphism detection. METHODS: This retrospective study was performed on 100 B-ALL patients (52 males and 48 females; age 4.6 ± 3.2 years) and 105 age- and sex-matched healthy controls (48 males and 57 females; age 5.1 ± 3 years). We used HRM to identify mir-612 rs12803915 genotypes. Sanger sequencing was applied to validate the HRM results. RESULTS: High resolution melting analysis was used to genotype the mir-612 rs12803915 polymorphism. We found no association between rs12803915 allele A and B-ALL risk in any inheritance models (p> 0.05). CONCLUSION: HRM is a suitable method to detect SNP rs12803915 in the mir-612 gene; however, we found no significant association between the rs12803915 polymorphism and ALL risk.
BACKGROUND: Acute lymphoblastic leukemia (ALL) is a highly heterogeneous malignancy that accounts for nearly 75% of leukemias in children. While the exact mechanism of ALL is not fully understood, some genetic variants have been implicated as associated with ALL susceptibility. The association between some genetic variants in miRNA genes and ALL risk has been described previously. A previous study suggested that mir-612 rs12803915 G> A may be associated with pediatric ALL risk. High-resolution melting (HRM) analysis is a reliable method that can be applied for polymorphism detection. METHODS: This retrospective study was performed on 100 B-ALL patients (52 males and 48 females; age 4.6 ± 3.2 years) and 105 age- and sex-matched healthy controls (48 males and 57 females; age 5.1 ± 3 years). We used HRM to identify mir-612 rs12803915 genotypes. Sanger sequencing was applied to validate the HRM results. RESULTS: High resolution melting analysis was used to genotype the mir-612 rs12803915 polymorphism. We found no association between rs12803915 allele A and B-ALL risk in any inheritance models (p> 0.05). CONCLUSION: HRM is a suitable method to detect SNP rs12803915 in the mir-612 gene; however, we found no significant association between the rs12803915 polymorphism and ALL risk.
Authors: Joseph L Wiemels; Kyle M Walsh; Adam J de Smith; Catherine Metayer; Semira Gonseth; Helen M Hansen; Stephen S Francis; Juhi Ojha; Ivan Smirnov; Lisa Barcellos; Xiaorong Xiao; Libby Morimoto; Roberta McKean-Cowdin; Rong Wang; Herbert Yu; Josephine Hoh; Andrew T DeWan; Xiaomei Ma Journal: Nat Commun Date: 2018-01-18 Impact factor: 17.694
Authors: Silvia Jiménez-Morales; Juan Carlos Núñez-Enríquez; Jazmín Cruz-Islas; Vilma Carolina Bekker-Méndez; Elva Jiménez-Hernández; Aurora Medina-Sanson; Irma Olarte-Carrillo; Adolfo Martínez-Tovar; Janet Flores-Lujano; Julian Ramírez-Bello; María Luisa Pérez-Saldívar; Jorge Alfonso Martín-Trejo; Héctor Pérez-Lorenzana; Raquel Amador-Sánchez; Felix Gustavo Mora-Ríos; José Gabriel Peñaloza-González; David Aldebarán Duarte-Rodríguez; José Refugio Torres-Nava; Juan Eduardo Flores-Bautista; Rosa Martha Espinosa-Elizondo; Pedro Francisco Román-Zepeda; Luz Victoria Flores-Villegas; Edna Liliana Tamez-Gómez; Víctor Hugo López-García; José Ramón Lara-Ramos; Juana Esther González-Ulivarri; Sofía Irene Martínez-Silva; Gilberto Espinoza-Anrubio; Carolina Almeida-Hernández; Rosario Ramírez-Colorado; Luis Hernández-Mora; Luis Ramiro García-López; Gabriela Adriana Cruz-Ojeda; Arturo Emilio Godoy-Esquivel; Iris Contreras-Hernández; Abraham Medina-Hernández; María Guadalupe López-Caballero; Norma Angélica Hernández-Pineda; Jorge Granados-Kraulles; María Adriana Rodríguez-Vázquez; Delfino Torres-Valle; Carlos Cortés-Reyes; Francisco Medrano-López; Jessica Arleet Pérez-Gómez; Annel Martínez-Ríos; Antonio Aguilar-De-Los-Santos; Berenice Serafin-Díaz; María de Lourdes Gutiérrez-Rivera; Laura Elizabeth Merino-Pasaye; Gilberto Vargas-Alarcón; Minerva Mata-Rocha; Omar Alejandro Sepúlveda-Robles; Haydeé Rosas-Vargas; Alfredo Hidalgo-Miranda; Juan Manuel Mejía-Aranguré Journal: Front Oncol Date: 2021-11-05 Impact factor: 6.244