Genome-wide analysis of gibberellin-dioxygenases gene family and their responses to GA applications in maize.

Gibberellin-dioxygenases genes plays important roles in the regulating plant development. However, Gibberellin-dioxygenases genes are rarely reported in maize, especially response to gibberellin (GA). In present study, 27 Gibberellin-dioxygenases genes were identified in the maize and they were classified into seven subfamilies (I-VII) based on phylogenetic analysis. This result was also further confirmed by their gene structure and conserved motif characteristics. And gibberellin-dioxygenases genes only occurred segmental duplication that occurs most frequently in plants. Furthermore, the gibberellin-dioxygenases genes showed different tissue expression pattern in different tissues and most of the gibberellin-dioxygenases genes showed tissue specific expression. Moreover, almost all the gibberellin-dioxygenases genes were significantly elevated in response to GA except for ZmGA2ox2 and ZmGA20ox10 of 15 gibberellin-dioxygenases genes normally expressed in leaves while 10 and 11 gibberellin-dioxygenases genes showed up and down regulated under GA treatment than that under normal condition in leaf sheath. In addition, we found that ZmGA2ox1, ZmGA2ox4, ZmGA20ox7, ZmGA3ox1 and ZmGA3ox3 might be potential genes for regulating balance of GAs which play essential roles in plant development. These findings will increase our understanding of Gibberellin-dioxygenases gene family in response to GA and will provide a solid base for further functional characterization of Gibberellin-dioxygenases genes in maize.

Introduction

The development of plant organs is directly dependent on the frequency of cell division, the parameters of the cell cycle, and the number and size of the cells [1]. Plants are continuously exposed to a variety of stress factors in their natural environment. Of them, gibberellins (GAs) play multiple roles in plant development and stress responses which will significantly affect the production and quality of the plants [2, 3]. To adapt natural environment, plants have to acclimate to GA by triggering a cascade of events leading to changes in gene expression and subsequently to biochemical and physiological modifications. GA synthesis, metabolism and GA signaling transduction play core roles to cope with various natural environment. However, although much effort, the key genes and signaling pathways involved in GA remains need for further study.

With development of advanced technologies, numbers of genes which contribute to GA signaling were discovered. Genetic analyses of GA-deficient and GA-response mutants have revealed that the central step in GA action is to turn off the repressive effects of DELLAs in plants. In the presence of GA, the GA-GID1-DELLA complex stimulates the interaction of DELLAs with an F-box protein, resulting in the degradation of DELLAs and consequently the activation of downstream-responsive processes [4]. In higher plants, the flux of active GAs is regulated by the balance between their rates of biosynthesis and deactivation. The GA 20-oxidase (GA20ox) and GA 3-oxidase (GA3ox) genes encode key enzymes of bioactive GAs synthesis, whereas GA 2-oxidase (GA2ox) is the major GA inactivation enzyme [5]. In fact, increasing numbers of studies have investigated the gibberellin oxidase gene family in various kinds of plants, such as rice, Arabidopsis, soybean, Grape and Phyllostachys edulis [6-8]. In addition, the function of several gibberellin-dioxygenases genes has been clarified. For example, Shan et al., demonstrated that OsGA2ox5 was involved in plant growth, the root gravity response and salt stress [9]. Gibberellin 20-oxidase promoted initiation and elongation of cotton fibers by regulating gibberellin synthesis [10] while Gibberellin 20-Oxidase dictated the flowering-runnering decision in Diploid Strawberry [11]. And overexpression of jatropha gibberellin 2-oxidase 6 (jcga2ox6) induced dwarfism and smaller leaves, flowers and fruits in Arabidopsis and Jatropha [12].

Maize is one of the most important cereal crops worldwide. GA has been showed play essential roles in response to environment stress during the development of maize. Yang et al., demonstrated that GA could improve the resistance of tebuconazole-coated maize seeds to chilling stress by microencapsulation [13]. Hu et al., found that GA promote brassinosteroids action and both increase heterosis for plant height [14] and Chen et al., considered that dwarfish and yield-effective GM maize could be developed through passivation of bioactive gibberellin [15]. Recently, Zhang and Wang demonstrated that GA signaling play important roles in response to phosphate deficiency and nitrogen uptake, respectively [16, 17]. In addition, increasing numbers of studies have demonstrated that numbers of genes involved in GA signaling which contribute to the development and the production of maize. For example. Wang et al., (2013) provided physiological and transcriptomic evidence that gibberellin biosynthetic deficiency was responsible for maize dominant dwarf11 (d11) mutant phenotype and they found that the expression of ent-kaurenoic acid oxidase (KAO), GA20ox and GA2ox are up-regulated in D11 [18]. Recently, some GA-responsive transcripts which encoded the components of GA pathway were showed differential expressed in wild type and D11 in response to gibberellin stimulation, including CPS, KS, and KO enzymes for GA biosynthesis, GA2ox enzymes for GA degradation, DELLA repressors and GID1 receptor for GA signaling [19]. Muylle et al., demonstrated that overexpression of GA20-OXIDASE1 impacts plant height, biomass allocation and saccharification efficiency in maize [20].

Taken together, these results demonstrated that the biosynthesis and deactivation of gibberellin-dioxygenases genes played essential roles in maize involved in GA induced growth and development. However, there is few systematic and complete investigation on gibberellin-dioxygenases genes family in maize. Therefore, in present study, we aimed to investigate the characteristics of the biosynthesis and deactivation of gibberellin-dioxygenases gene family and identify the key genes in response to GA in maize.

Materials and methods

Plant materials and GA treatments

Seeds of the maize (Zea mays L.) are disinfected with 2% sodium hypochlorite (NaClO) or 70% ethanol and then rinsed with distilled water three times. And the seeds then were grown in a greenhouse at 28°C/23°C(day/night) with a 16-h light/8-h dark photoperiod. For gibberellin (GA) treatment, seedlings were treated with 150mg/L GA with spraying to the leaves at two leaves and one heart period. During the period of GA treatment, the seedlings were watered every day, and control seedlings were maintained under non-stress conditions. After treatment for 6, 12, 24, 48, 72 h, the samples were collected and immediately frozen in liquid nitrogen and stored at -80°C for RNA isolation. The seedlings without GA treatment at 0 h act as control. There were three biological replicates for each experiment.

Identification of gibberellin-dioxygenases genes in maize

The Hidden Markov Model (HMM) profile of gibberellin-dioxygenases gene (accession number PF03171.20) was downloaded from the Pfam database (http://pfam.xfam.org/). All gibberellin-dioxygenases genes were obtained by screening protein sequences of maize using HMMER 3.0 software (http://hmmer.janelia.org/) and blastp (National Center for Biotechnology Information (NCBI) Basic Local Alignment Search Tool, e-value < = 0.001). The putative gibberellin-dioxygenases genes were checked by the NCBI Conserved domain database (CDD) and Simple Modular Architecture Research Tool (SMART) online. The gibberellin-dioxygenases genes from Arabidopsis and rice were download from TAIR (Arabidopsis Information Resource, https://www.arabidopsis.org/) and Rice Genome Annotation Project Database (http://rice.plantbiology.msu.edu/ respectively).

Characteristics of gibberellin-dioxygenases genes in maize

Both genome and coding sequences of gibberellin-dioxygenases genes were downloaded from the whole genome of maize (B73-REFERENCE-GRAMENE-4.0) database (https://alpha.maizegdb.org/). For gene structure analysis, genomic and CDS sequences were used for drawing gene structure schematic diagrams with the Gene Structure Display Server from the Center for Bioinformatics at Peking University (http://gsds.cbi.pku.edu.cn/index.php). Isoelectric point (PI) and Molecular weight (MW) of the gibberellin-dioxygenases proteins were analyzed by EXPASY website tool (https://web.expasy.org/compute_pi/). The map of the chromosome location with genes was constructed through the online software MapGene2Chrom web v2. Species-wide gene replication events was performed by using MCScanX.

Conserved motif distributions and phylogenetic analysis

Conserved motifs for each gibberellin-dioxygenases amino acid sequence were analyzed by Multiple Em for Motif Elicitation online software (MEME, http://meme-suite.org/tools/meme). Amino acid sequences of gibberellin-dioxygenases genes were used to build the phylogenetic tree. Prottest was firstly use to predict the best evolution model and JTT+G+I+F as the best evolution model to build the evolution tree using RAxML 1000 bootstrap and the phylogenetic tree visualization is done using Figtree software.

Tissue specific and GA induced expression analysis in maize

RNA-Seq datasets for tissue and GA treatment were downloaded from the NCBI sequence read archive (SRA) database (PRJNA314400 and PRJNA421076, respectively) [19, 21], then used to analyze the expression profiles of the identified gibberellin-dioxygenases genes. A total of 23 tissues spanning vegetative and reproductive stages of maize development, as well as GA treatments were used to identify tissue-specific or GA responsive ones. Trimmomatic was used to remove the sequencing adapters and low-quality reads; Clean reads were aligned to the reference genome by Hisat2 and Htseq was used to calculate the counts of the reads that aligned to the genome. And TPM was used to homogenize the gene expression data. After the expression data of tissue expression in maize is transformed by zscore, it is displayed on the iTOL online tool together with the motif information; The differential expressed gene analzed by using DESeq2.

Quantitative reverse transcription polymerase chain reaction (qRT-PCR)

Total RNA was extracted from tissues by using RNAprep pure Plant Kit (DP432, TIANGEN). 2 ug RNA was used to synthesize cDNA using PrimeScript™ RT reagent Kit with gDNA Eraser (RR047A, Takara, Japan) according to the manufacturer instructions. qRT-PCR was performed using ABI 7500 instrument (ABI7500, ABI, Foster City, CA, USA) with Geneseed® qPCR SYBR® Green Master Mix (Geneseed) with 20 μL reaction mixture of volume. The reaction volume consists of 10 μL SYBR® Green Master Mix, 0.5 μL of each primer (10 μM), 2 μL of the cDNA template, and 8 μL of RNase free H2O. Thermal cycling parameters for the amplification were as follows: 95°C, 5 min, followed by 40 cycles at 95°C,10 s and 60°C, 34 s. The expression level of gibberellin-dioxygenases genes were calculated by 2-△△Ct methods. Actin act as internal reference. Primers used in the present study were synthesized by BGI and the detailed information was listed in .

Statistical analysis

All the data from more than three biological repeats was analyzed using the SPSS 21.0 (SPSS, Inc., Chicago, IL, USA) software. Quantitative data was presented as mean ± SD. The significance of differences between normal group and GA treatment group were assessed by the paired t test. Significant differences were finally defined as P < 0.05.

Results

Based on the genome and transcriptome databases, candidate gibberellin-dioxygenases genes were explored through searching against genome of maize using HMMSearch (PF03171.20) and BLASTP (e-value < = 0.001) methods. Totally, 38 candidate gibberellin-dioxygenases were obtained in maize. After removing redundant sequences and confirming the presence of gibberellin-dioxygenases domains by MEME, 27 Gibberellin-dioxygenases genes were finally retained and used for further analysis, including 13 GA2ox1 genes (ZmGA2ox1-13), 11 GA20ox genes (ZmGA20ox1-11) and 3 GA3ox (ZmGA3ox1-3) genes, respectively. Further analysis showed that these gibberellin-dioxygenases genes varied from 903 (ZmGA20ox4) to 1392 (ZmGA20ox10) nucleic acid in length () and the exon numbers were 0 or 3 (). Their molecular weight ranged from 32.3 kDa (ZmGA20ox4) to 50.7 kDa (ZmGA20ox10) and the PI ranged from 5.1 (ZmGA2ox5) to 8.91 (ZmGA2ox9), suggesting that 37 Gibberellin-dioxygenases might play different roles involved in different processes in maize ().

Gene structure of gibberellin-dioxygenases genes in maize.

For gene structure analysis, genomic and CDS sequences were used for drawing gene structure schematic diagrams with the Gene Structure Display Server from the Center for Bioinformatics at Peking University (http://gsds.cbi.pku.edu.cn/index.php).

Chromosome distribution of gibberellin-dioxygenases genes in maize

Generally, genes often undergo replication events during evolution. In order to know whether gibberellin-dioxygenases genes also experienced gene replication events, the chromosome distribution of 27 gibberellin-dioxygenases genes were analyzed. The results showed that these genes were distributed in 10 chromosomes, except for chromosome 6. And chromosome 6 contained the most gibberellin-dioxygenases genes (6 genes). Further analysis showed that the gibberellin-dioxygenases genes only occurred fragment duplication while there were no tandem duplication events observed ().

Chromosome distribution for gibberellin-dioxygenases genes in maize.

The chromosome distribution was finished by MapGen2Chrom web V2(http://mg2c.iask.in/mg2c_v2.0/).

Phylogenetic analysis of gibberellin-dioxygenases genes in maize

To investigate the phylogenetic relationships of the gibberellin-dioxygenases gene family in maize, 27 Gibberellin-dioxygenases genes in maize, together with 16 Arabidopsis and 22 rice gibberellin-dioxygenases genes (), were selected for phylogenetic analysis. As shown, the gibberellin-dioxygenases proteins were clustered into seven groups, I to VII. There are 7, 3, 6, 4, 3, 2 and 2 gibberellin-dioxygenases genes were in I to IV groups, respectively. And further analysis showed that in every groups contained gibberellin-dioxygenases genes from Arabidopsis and rice, indicating that the differentiation of gibberellin-dioxygenases genes in maize is earlier than that of monocotyledonous and dicotyledonous plants.

Phylogenetic analysis of gibberellin-dioxygenases proteins among maize (27), Arabidopsis (16) and rice (22).

The phylogenetic tree was constructed based on the full-length protein sequences using Figtree software. Seven subgroups (I-VII) are shown in various colors.

Conserved motifs analysis of gibberellin-dioxygenases genes in maize

The conserved motifs of gibberellin-dioxygenases protein sequences were further predicted using the MEME software. A total of 10 conserved motifs were found among all the gibberellin-dioxygenases genes (). In consistent with phylogenetic tree of gibberellin-dioxygenases genes, the 27 gibberellin-dioxygenases genes were classified to 7 clades. Further analysis showed that all the gibberellin-dioxygenases proteins were lack of 1-motifs in I-IV clades. Of them, the proteins from clades I and II is lack of motif 9. The genes form Clade III, V and ZmGA3ox2 from Clade VII showed the similar motifs constitution which lack of motif 9 and motif 8 except for ZmGA3ox3 and ZmGA2ox6 from Clade III and ZmGA20ox8 from Clade V. The genes from clade IV and ZmGA3ox1 from Clade VII showed the same motifs constitution which lack of motif 8 and motif 10. In addition, the ZmGA20ox9 and from ZmGA20ox9 showed the greatest degree of absence in conserved motifs that lack of 5 and 3 motifs, respectively. The results of the conserved motifs of gibberellin-dioxygenases genes showed evolutionary divergence in maize.

Phylogenetic relationships and conserved motifs compositions of the 27 gibberellin-dioxygenases genes in maize.

MEME was used to predict conserved motifs. Each motif is represented by a different colored box. Amino acid sequences of gibberellin-dioxygenases genes were used to build the phylogenetic tree. Prottest was firstly use to predict the best evolution model and JTT+G+I+F as the best evolution model to build the evolution tree using RAxML 1000 bootstrap and the phylogenetic tree visualization is done using Figtree software.

Tissue-specific expression profile analysis of gibberellin-dioxygenases genes in maize

We have demonstrated that the gibberellin-dioxygenases genes showed different conserved motifs. In order to insight into the putative functions of the gibberellin-dioxygenases genes in maize, the temporal and spatial expression profile of these identified gibberellin-dioxygenases genes were analyzed using the public RNA-Seq data (PRJNA314400) from different tissues (). As shown, the gibberellin-dioxygenases genes showed different tissue expression pattern in different tissues and most of the gibberellin-dioxygenases genes showed tissue specific expression. For example, ZmGA3ox1 mainly expressed in the germinating period of kemels while ZmGA3ox3 and ZmGA20ox11 were mainly expressed in tip of the roots. In addition, we also found several gibberellin-dioxygenases were simultaneously expressed in the same tissue, such as ZmGA20ox5, ZmGA20ox2 and ZmGA2ox5 expressed in silks and ZmGA2ox6, ZmGA20ox3, ZmGA20ox4 and ZmGA20ox1expressed in the transfer zone of matemal. The diversity of tissue expression pattern indicated the functional diversity of gibberellin-dioxygenases genes which will contribute to different morphogenesis in plant development.

Expression analysis of gibberellin-dioxygenases genes responding to GA

A large number of gibberellin-dioxygenases genes have been demonstrated to regulate numbers of processes in response to GA. However, the studies focus on the gibberellin-dioxygenases in response to GA in maize is rare. Therefore, transcriptome of maize (PRJNA421076) in response to GA were used to explore the GA induced expression of gibberellin-dioxygenases genes in leaf and leaf sheath. As shown, almost all the gibberellin-dioxygenases genes were significantly elevated in response to GA except for ZmGA2ox2 and ZmGA20ox10 of 15 gibberellin-dioxygenases genes normally expressed in leaves. 10 and 11 gibberellin-dioxygenases genes showed up and down regulated under GA treatment than that under normal condition in leaf sheath. Further analysis showed that these differential expressed genes were from different groups, implying that gibberellin-dioxygenases genes might play different roles in response to GA.

Candidate gibberellin-dioxygenases genes response to GA verified by qRT-PCR in maize

In order to explore the key GA stress-responsive candidates in maize, 6 Gibberellin-dioxygenasess based on the RNA-Seq data which showed the most significant upregulated in leaves or leaf sheath were selected to verified by qRT-PCR analysis at 6h, 12h, 24h, 48h and 72h after GA treatment. In consistent with the RNA-seq data, compared with control, the expression of ZmGA2ox1, ZmGA2ox4, ZmGA20ox2 was significantly elevated in 6h and 24h, 6h and 12h, and 24h, respectively (. However, the expression of ZmGA20ox7, ZmGA3ox1 and ZmGA3ox3 were significantly downregulated at all the times after GA treatment compared with control. This result may be caused by different varieties used in present. These results demonstrated ZmGA2ox1, ZmGA2ox4 and ZmGA20ox2 could consider to be key genes which played vital roles in GA stress.

The expression of candidate genes that were most significantly expressed in the response to GA using qRT-PCR analysis.

A-F, The expression of ZmGA2ox1, ZmGA2ox4, ZmGA20ox7, ZmGA3ox1 and ZmGA3ox3 after GA treatment, respectively. CK, Control check. **, p< 0.01, Student’s t-test). Gene expression profiles were evaluated using the 2-△△Ct methods.

Discussion

Gibberellin (GA) is an essential hormone that is involved in many aspects of plant growth and development, including seed maturation, stem elongation and response to abiotic stress [22, 23]. Gibberellin-dioxygenases genes are reported to be involved in many critical development processes [24]. Systematic and integrative analyses of gibberellin-dioxygenases genes have been performed in Arabidopsis, rice and some other plants [5]. However, the gibberellin-dioxygenases genes in response to GA are less studied in maize compared with that in Arabidopsis and rice. Therefore, we sought to study the characteristics of this gene family in response to gibberellin by combining bioinformatic and expression analyses.

The details of how GAs is biosynthesis and deactivation have accumulated in the last few years and are beginning to explain in molecular terms the pleiotropic action of GA in plant development [4, 5]. The 2-oxoglutarate dependent dioxygenases (2-ODDs), including GA20ox and GA3ox, are the key enzymes in a series of oxidation steps and, GA 2-oxidases GA2ox are the unique enzymes in the pathways and regulation of GA degradation [2]. And several gibberellin-dioxygenases genes were also investigated in maize, such as ga2ox1 [25]. In present study, 27 Gibberellin-dioxygenases genes were finally retained and used for further analysis, including 13 GA2ox1 genes, 11 GA20ox genes and 3 GA3ox (ZmGA3ox1-3) genes which is different from the numbers of other plants, such as 16 members in Arabidopsis thaliana [26], 21 members in rice [27], 24 members in soybean [8]. Gene duplications are considered to be one of the primary driving forces in the evolution of genomes and genetic systems [28]. Segmental and tandem duplications have been suggested to represent two of the main causes of gene family expansion in plants [29]. Further analysis showed that 27 gibberellin-dioxygenases genes were distributed in 10 chromosomes, except for chromosome 6. And gibberellin-dioxygenases genes only occurred segmental duplication while there were no tandem duplication events. These results are consistent with that segmental duplications multiple genes through polyploidy followed by chromosome rearrangements and occurs most frequently in plants because most plants are diploidized polyploids and retain numerous duplicated chromosomal blocks within their genomes [30]. Previous investigations of the gibberellin-dioxygenases genes in various plant species have divided the plant gibberellin-dioxygenases genes into different classes [5]. In present study, the gibberellin-dioxygenases proteins were clustered into seven groups, I to VII. And further analysis showed that in every groups contained gibberellin-dioxygenases genes from Arabidopsis and rice, indicating that the differentiation of gibberellin-dioxygenases genes in maize is earlier than that of monocotyledonous and dicotyledonous plants. Specific motifs in amino acid sequences are vital regions related to function. Previous analysis found that all the GA20ox, GA3ox and GA2ox sequences belonged to the 2-ODDs superfamily, which share high homology with the functional domains DIOX_N (PF14226) and 2OG-FeII_Oxy (PF03171). In consistent with phylogenetic tree of gibberellin-dioxygenases genes, the 27 gibberellin-dioxygenases genes were classified to 7 clades. Further analysis showed that all the gibberellin-dioxygenases proteins were lack of 1–4 motifs in I-IV clades. The results of the conserved motifs of gibberellin-dioxygenases genes showed evolutionary divergence in maize, suggesting the divergent function in maize development of gibberellin-dioxygenases genes.

In order to investigated the divergent function caused by the conserved motifs, the expression of gibberellin-dioxygenases genes was investigated. The gibberellin-dioxygenases genes showed different tissue expression pattern in different tissues and most of the gibberellin-dioxygenases genes showed tissue specific expression. In fact, the gibberellin-dioxygenases genes from different plants have been studied [7]. And they played various kinds of functions in different plants, such as response to abiotic stress, increased biomass production and yield and plant development. For example, activation of gibberellin 2-oxidase 6 decreased active gibberellin levels and created a dominant semi-dwarf phenotype in rice (Oryza sativa L.) [31]. Overexpression of stga2ox1 gene increases the tolerance to abiotic stress in transgenic potato plants [32]. Developing xylem-preferential expression of PdGA20ox1 improves woody biomass production in a hybrid poplar [33] and the QTL GNP1 encodes GA20ox1, which increases grain number and yield by increasing cytokinin activity in rice panicle meristems [34]. In addition, several gibberellin-dioxygenases genes have been clarified. Such as maize dominant dwarf11 (d11) mutant phenotype is related to the upregulation of GA20ox and GA2ox which contribute to gibberellin biosynthetic deficiency [18]. Muylle et al., demonstrated that overexpression of GA20-OXIDASE1 impacts plant height, biomass allocation and saccharification efficiency in maize [20]. And expression of ZmGA20ox cDNA alters plant morphology and increases biomass production of switchgrass (Panicum virgatum L.) [35]. Furthermore, it also showed that the maize transcription factor KNOTTED1 directly regulated the gibberellin catabolism gene ga2ox1 [25]. Taken together, the diversity of tissue expression pattern indicated the functional diversity of gibberellin-dioxygenases genes which will contribute to different morphogenesis in plant development and response to abiotic stress.

Wang et al., found some GA2ox, GA3ox, and GA20ox genes which showed differential expressed after GA treatment [19, 36]. In present study, almost all the gibberellin-dioxygenases genes were significantly elevated in response to GA except for ZmGA2ox2 and ZmGA20ox10 of 15 gibberellin-dioxygenases genes normally expressed in leaves. And 10 and 11 gibberellin-dioxygenases genes showed up and down regulated under GA treatment than that under normal condition in leaf sheath. Further analysis showed that these differential expressed genes were from different groups, implying that gibberellin-dioxygenases genes might play different roles in response to GA. Generally, in most plants, GA20ox and GA3ox which contribute to the production of bioactive GAs are downregulated by applied exogenous GA [37]. In contrast, the genes encoding GA2ox, which convert active GAs to inactive catabolites, are upregulated by GA treatment [38]. qRT-PCR results showed that compared with control, the expression of ZmGA2ox1and ZmGA2ox4 was significantly elevated in 6h and 24h, 6h and 12h, respectively. However, the expression of ZmGA20ox7, ZmGA3ox1 and ZmGA3ox3 were significantly downregulated at all the times after GA treatment while ZmGA20ox2 was significantly elevated at 24h compared with control. Our findings are in accordance with previous studies [39]. These results indicated that ZmGA2ox1, ZmGA2ox4, ZmGA20ox7, ZmGA3ox1 and ZmGA3ox3 might be potential genes for regulating balance of GAs which play essential roles in plant development. However, the precise function and mechanism of these candidate genes need to be further investigation.

Conclusion

Our results provide a more comprehensive understanding of gibberellin-dioxygenases in maize, including phylogenetic analysis, gene structure and conserved motif characteristics, gene duplication and tissue expression. Totally, 27 Gibberellin-dioxygenases genes were identified which classified into seven subfamilies (I-VII) based on phylogenetic analysis, gene structure and conserved motif characteristics. And gibberellin-dioxygenases genes only occurred segmental duplication that occurs most frequently in plants. Furthermore, the diversity of tissue expression pattern indicated the functional diversity of gibberellin-dioxygenases genes which will contribute to different morphogenesis in plant development. Moreover, ZmGA2ox1, ZmGA2ox4, ZmGA20ox7, ZmGA3ox1 and ZmGA3ox3 were considered to be potential genes for regulating balance of GAs which play essential roles in plant development though transcriptome data and qRT-PCR. Our findings provided a basis for conducting in-depth mechanistic studies on the in distinct biological characteristics and adaptability in response to GA for gibberellin-dioxygenases genes in maize.

Primers used in present study.

(DOCX)

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Gibberellin-dioxygenases genes in Arabidopsis, rice and maize.

(XLSX)

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Tissue expression profiles for gibberellin-dioxygenases genes in maize.

(XLSX)

Click here for additional data file.

12 Oct 2020

PONE-D-20-27828

Genome-wide analysis of the biosynthesis and deactivation of gibberellin-dioxygenases gene family and key genes identified in response to GA in maize

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Reviewer #1: Yes

Reviewer #2: Yes

**********

4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: No

**********

5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Ci et al. identified 27 gibberellin-dioxygenase proteins that are involved in GA biosynthesis and degradation in maize. The authors performed bioinformatic analysis and determined transcription levels of tested genes. Finally, five genes were speculated as potential genes for regulating GA concentrations, which may play essential roles in maize development. I only have minor comment to this study.

1. Abstract: line 14, “Gas” should be “GAs”. Please check the whole manuscript carefully and correct the mistake like this.

2. The names of all genes in the whole manuscript and figures should be italicized.

3. Part 2.1: the method is not described in sufficient detail. How did the authors collect control samples? Did you collect control samples after water treatment (no GAs) for 6, 12, 24, 48, and72 h? If so, why is this data not shown in Figure 7? If you only collected control sample at 0 h (before GA treatment), when you collected GA-treated samples at 6, 12, 24, 48, and72 h, you found some gene expressions were changed, how to exclude the photoperiod and temperature effect on these gene expression?

4. In addition, in figure 7, “CK” is the abbreviation of cytokinin in english, please correct.

5. Part 2.6: line 7: H2O should be H2O; line 10, what is the meaning of “XX”?

6. The third paragraph of the discussion is not enough because many gibberellin-dioxygenase genes have been functional studied in many other plants, such as Arabidopsis and rice.

7. Reference: some journal are full name, but some are abbreviation.

Reviewer #2: In this manuscript, the authors reported Genome-wide identification of gibberellin-dioxygenases gene family in maize. By employing sequence-based bioinformatic analysis and qRT-PCR based gene expression determination, 27 Gibberellin-dioxygenases genes were identified in maize. The similar research had been done a lot in other plant species. From this prospective, this study in maize is lack of novelty. Still, it represents a standard report in relevant fields. I would like to suggest an acceptance after revision.

1.In the context of reported data in this study, the title of this manuscript is of over-presentation. Should the following be better: Genome-wide analysis of gibberellin-dioxygenases gene family and their responses to GA applications in maize

2.Too much language flaws in tense, Singular/plural, and grammar. For instance in the abstract, Gibberellin-dioxygenases genes “plays”; In “the” present study; their “structures”; different expression “patterns”; tissue-specific, and so on. In the main text, for example, “In order to investigated”, wrong tense. Any way, it should be very easy to go through these by carefully self-reading. Even that, you may choose professional language editing services. IT MATTERS.

3.Some presentation are inexact, for instance:

(a)“ However, although much effort, the key genes and signaling pathways involved in GA remains elusive. “ The authors should define the context or put suitable limiting word in sentences like this one. In this case, at least the work in Arabidopsis, rice, and some others have accomplished a lot. Therefore, we know the key genes, the main pathways, and accordingly, we can refer to study their conserved counterparts in maize. So on so forth.

(b) “However, there is no systematic and complete investigation on gibberellin-dioxygenases genes family.” No, there have been quite a lot publications on different species. Should you please check and include as references? List below as examples:

Li, C., et al. (2019). "Comprehensive expression analysis of Arabidopsis GA2-oxidase genes and their functional insights." Plant Sci 285: 1-13.

Yan, J., et al. (2017). "Ectopic expression of GA 2-oxidase 6 from rapeseed (Brassica napus L.) causes dwarfism, late flowering and enhanced chlorophyll accumulation in Arabidopsis thaliana." Plant Physiol Biochem 111: 10-19.

Hu, Y.-X., Y.-B. Tao and Z.-F. Xu (2017). "Overexpression of Jatropha Gibberellin 2-oxidase 6 (JcGA2ox6) induces dwarfism and smaller leaves, flowers and fruits in Arabidopsis and Jatropha." Front Plant Sci 8: 2103.

Shan, X., Y. Li, et al. (2013). "Transcriptome Profile Analysis of Maize Seedlings in Response to High-salinity, Drought and Cold Stresses by Deep Sequencing." Plant Molecular Biology Reporter 31(6): 1485-1491.

Gou, J., S. H. Strauss, et al. (2010). "Gibberellins regulate lateral root formation in Populus through interactions with auxin and other hormones." Plant Cell 22(3): 623-639.

Yamaguchi, S. (2008). Gibberellin metabolism and its regulation. Annual Review of Plant Biology. Palo Alto, Annual Reviews. 59: 225-251.

(b)“the gibberellin-dioxygenases genes in response to GA are still poorly understood”, why? Too vague description.

**********

6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: No

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

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21 Feb 2021

Dear Editor:

On behalf of my co-authors, we thank you very much for giving us an opportunity to revise our manuscript, we appreciate editor and reviewers very much for their positive and constructive comments and suggestions. We have studied reviewer’s comments carefully and have made revision which marked in red in the paper. We have tried our best to revise our manuscript according to the comments. And the revised contents as follows:

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

________________________________________

2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: N/A

________________________________________

3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

________________________________________

4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: No

________________________________________

5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Ci et al. identified 27 gibberellin-dioxygenase proteins that are involved in GA biosynthesis and degradation in maize. The authors performed bioinformatic analysis and determined transcription levels of tested genes. Finally, five genes were speculated as potential genes for regulating GA concentrations, which may play essential roles in maize development. I only have minor comment to this study.

1. Abstract: line 14, “Gas” should be “GAs”. Please check the whole manuscript carefully and correct the mistake like this.

Thanks for your comments, we have carefully checked the whole manuscript carefully and correct the mistakes in the manuscript.

2. The names of all genes in the whole manuscript and figures should be italicized.

Thanks for your comments, the names of all genes in the whole manuscript and figures have been italicized.

3. Part 2.1: the method is not described in sufficient detail. How did the authors collect control samples? Did you collect control samples after water treatment (no GAs) for 6, 12, 24, 48, and72 h? If so, why is this data not shown in Figure 7? If you only collected control sample at 0 h (before GA treatment), when you collected GA-treated samples at 6, 12, 24, 48, and72 h, you found some gene expressions were changed, how to exclude the photoperiod and temperature effect on these gene expression?

4. In addition, in figure 7, “CK” is the abbreviation of cytokinin in english, please correct.

Thanks for your comments, we have changed it in the manuscript.

5. Part 2.6: line 7: H2O should be H2O; line 10, what is the meaning of “XX”?

Thanks for your comments, we have revised it in the manuscript.

6. The third paragraph of the discussion is not enough because many gibberellin-dioxygenase genes have been functional studied in many other plants, such as Arabidopsis and rice.

Thanks for your comments, we have changed it in the manuscript.

7. Reference: some journal are full name, but some are abbreviation.

Thanks for your comments, we have revised the references as the format of PLOS one.

Reviewer #2: In this manuscript, the authors reported Genome-wide identification of gibberellin-dioxygenases gene family in maize. By employing sequence-based bioinformatic analysis and qRT-PCR based gene expression determination, 27 Gibberellin-dioxygenases genes were identified in maize. The similar research had been done a lot in other plant species. From this prospective, this study in maize is lack of novelty. Still, it represents a standard report in relevant fields. I would like to suggest an acceptance after revision.

1.In the context of reported data in this study, the title of this manuscript is of over-presentation. Should the following be better: Genome-wide analysis of gibberellin-dioxygenases gene family and their responses to GA applications in maize

Thanks for your comments, the title have been changed to “Genome-wide analysis of gibberellin-dioxygenases gene family and their responses to GA applications in maize”.

2.Too much language flaws in tense, Singular/plural, and grammar. For instance in the abstract, Gibberellin-dioxygenases genes “plays”; In “the” present study; their “structures”; different expression “patterns”; tissue-specific, and so on. In the main text, for example, “In order to investigated”, wrong tense. Any way, it should be very easy to go through these by carefully self-reading. Even that, you may choose professional language editing services. IT MATTERS.

Thanks for your comments, we have chosen professional language editing service to revised the manuscript.

3. Some presentation are inexact, for instance:

(a)“ However, although much effort, the key genes and signaling pathways involved in GA remains elusive. “ The authors should define the context or put suitable limiting word in sentences like this one. In this case, at least the work in Arabidopsis, rice, and some others have accomplished a lot. Therefore, we know the key genes, the main pathways, and accordingly, we can refer to study their conserved counterparts in maize. So on so forth.

Thanks for your comments, we have changed it in the manuscript.

(b) “However, there is no systematic and complete investigation on gibberellin-dioxygenases genes family.” No, there have been quite a lot publications on different species. Should you please check and include as references? List below as examples:

Li, C., et al. (2019). "Comprehensive expression analysis of Arabidopsis GA2-oxidase genes and their functional insights." Plant Sci 285: 1-13.

Yan, J., et al. (2017). "Ectopic expression of GA 2-oxidase 6 from rapeseed (Brassica napus L.) causes dwarfism, late flowering and enhanced chlorophyll accumulation in Arabidopsis thaliana." Plant Physiol Biochem 111: 10-19.

Hu, Y.-X., Y.-B. Tao and Z.-F. Xu (2017). "Overexpression of Jatropha Gibberellin 2-oxidase 6 (JcGA2ox6) induces dwarfism and smaller leaves, flowers and fruits in Arabidopsis and Jatropha." Front Plant Sci 8: 2103.

Shan, X., Y. Li, et al. (2013). "Transcriptome Profile Analysis of Maize Seedlings in Response to High-salinity, Drought and Cold Stresses by Deep Sequencing." Plant Molecular Biology Reporter 31(6): 1485-1491.

Gou, J., S. H. Strauss, et al. (2010). "Gibberellins regulate lateral root formation in Populus through interactions with auxin and other hormones." Plant Cell 22(3): 623-639.

Yamaguchi, S. (2008). Gibberellin metabolism and its regulation. Annual Review of Plant Biology. Palo Alto, Annual Reviews. 59: 225-251.

Thanks for your comments, we have added these references in the manuscript.

(b)“the gibberellin-dioxygenases genes in response to GA are still poorly understood”, why? Too vague description.

Thanks for your comments, we have changed it in the manuscript.

________________________________________

6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: No

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step.

________________________________________

In compliance with data protection regulations, you may request that we remove your personal registration details at any time. (Remove my information/details). Please contact the publication office if you have any questions.

Submitted filename: rebuttal letter(1)(1).docx

Click here for additional data file.

5 Mar 2021

PONE-D-20-27828R1

Genome-wide analysis of gibberellin-dioxygenases gene family and their responses to GA applications in maize

PLOS ONE

Dear Dr. yang,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised by reviewer #1.

Please submit your revised manuscript by Apr 19 2021 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

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We look forward to receiving your revised manuscript.

Kind regards,

Keqiang Wu, Ph.D

Academic Editor

PLOS ONE

Journal Requirements:

Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: (No Response)

Reviewer #2: All comments have been addressed

**********

2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: No

Reviewer #2: N/A

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: I do not agree to accept the manuscript immediately unless the authors clearly answer my query. 1. In my previous comments, i asked how did the authors collect control samples in Part 2.1? The authors answered: “The seedlings without GA treatment at 6, 12, 24, 48, 72 h act as control.” If so, adding GA treated samples at 6, 12, 24, 48, 72 h, there should be ten samples. Therefore, ten expression data should be showed in Fig 7, but there is only one control and five GA treated samples in the revised manuscript. Why? How did the authors process the expression data? Please describe it in sufficient detail.

2.In my previous comments, i said: “in figure 7, “CK” is the abbreviation of cytokinin in english, please correct.” The authors answered “Thanks for your comments, we have changed it in the manuscript.” However, i can not see the change in figure 7 of the revised manuscript.

3.“There were at least three biological replicates for each experiment”? I want to know the exact number of replicates.

Reviewer #2: The authors answered some of their responses inadequately. But it does not matter. They did their best. This is a good work of reference to the maize community. At the end, I consider an acceptance to this manuscript.

**********

7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: No

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step.

31 Mar 2021

On behalf of my co-authors, we thank you very much for giving us an opportunity to revise our manuscript, we appreciate editor and reviewers very much for their positive and constructive comments and suggestions. We have studied reviewer’s comments carefully and have made revision which marked in red in the paper. We have tried our best to revise our manuscript according to the comments. And the revised contents as follows:

Reviewer #1: I do not agree to accept the manuscript immediately unless the authors clearly answer my query. 1. In my previous comments, i asked how did the authors collect control samples in Part 2.1? The authors answered: “The seedlings without GA treatment at 6, 12, 24, 48, 72 h act as control.” If so, adding GA treated samples at 6, 12, 24, 48, 72 h, there should be ten samples. Therefore, ten expression data should be showed in Fig 7, but there is only one control and five GA treated samples in the revised manuscript. Why? How did the authors process the expression data? Please describe it in sufficient detail.

Thanks for your comments, we have verified our data again, the seedlings without GA treatment at 0h act as control which presented as one control and five GA treated samples. And we have revised it in the revised manuscript.

2.In my previous comments, i said: “in figure 7, “CK” is the abbreviation of cytokinin in english, please correct.” The authors answered “Thanks for your comments, we have changed it in the manuscript.” However, i can not see the change in figure 7 of the revised manuscript.

Thanks for your comments, CK is the abbreviation of “Control check”not cytokinin.We have explain it in the figure legends of Fig.7.

3.“There were at least three biological replicates for each experiment”? I want to know the exact number of replicates.

Thanks for your comments, the exact number of replicates is three.

Reviewer #2: The authors answered some of their responses inadequately. But it does not matter. They did their best. This is a good work of reference to the maize community. At the end, I consider an acceptance to this manuscript.

Thanks for your comments,

7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: No

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step.

In compliance with data protection regulations, you may request that we remove your personal registration details at any time. (Remove my information/details). Please contact the publication office if you have any questions.

6 Apr 2021

Genome-wide analysis of gibberellin-dioxygenases gene family and their responses to GA applications in maize

PONE-D-20-27828R2

Dear Dr. yang,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Keqiang Wu, Ph.D

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

13 Apr 2021

PONE-D-20-27828R2

Genome-wide analysis of gibberellin-dioxygenases gene family and their responses to GA applications in maize

Dear Dr. Yang:

I'm pleased to inform you that your manuscript has been deemed suitable for publication in PLOS ONE. Congratulations! Your manuscript is now with our production department.

If your institution or institutions have a press office, please let them know about your upcoming paper now to help maximize its impact. If they'll be preparing press materials, please inform our press team within the next 48 hours. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information please contact onepress@plos.org.

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Thank you for submitting your work to PLOS ONE and supporting open access.

Kind regards,

PLOS ONE Editorial Office Staff

on behalf of

Professor Keqiang Wu

Academic Editor

PLOS ONE

Table 1

Characteristic of gibberellin-dioxygenases genes in maize.

geneName	Gene	Transcript	Chrom	Start	End	Strand	Length of CDS	Length of peptide	PI	MW
ZmGA2ox1	Zm00001d002999	Zm00001d002999_T001	2	29175293	29178113	+	1089	362	6.66	38914.2
ZmGA2ox1	Zm00001d002999	Zm00001d002999_T002	2	29175294	29176187	+	420	139	8.77	14767.8
ZmGA2ox2	Zm00001d039394	Zm00001d039394_T001	3	3274307	3276532	+	996	331	5.83	35103.8
ZmGA2ox3	Zm00001d040737	Zm00001d040737_T001	3	60952847	60960982	-	1050	349	8.54	36939
ZmGA2ox4	Zm00001d043411	Zm00001d043411_T001	3	199019233	199021284	-	996	331	8.3	35497.3
ZmGA2ox5	Zm00001d017294	Zm00001d017294_T001	5	192007580	192011428	-	1086	361	5.1	39407.4
ZmGA2ox5	Zm00001d017294	Zm00001d017294_T002	5	192009900	192011331	-	579	192	4.43	20599.7
ZmGA2ox6	Zm00001d035994	Zm00001d035994_T001	6	65164556	65165566	-	1011	336	6.13	35937.5
ZmGA2ox7	Zm00001d037565	Zm00001d037565_T001	6	129802297	129807428	-	618	205	6.23	22653.8
ZmGA2ox7	Zm00001d037565	Zm00001d037565_T002	6	129802305	129807398	-	1101	366	8.83	39675.8
ZmGA2ox7	Zm00001d037565	Zm00001d037565_T003	6	129802324	129807396	-	618	205	6.23	22653.8
ZmGA2ox8	Zm00001d037724	Zm00001d037724_T001	6	135240118	135243717	+	453	150	11.5	15765.7
ZmGA2ox8	Zm00001d037724	Zm00001d037724_T002	6	135240119	135243717	+	1002	333	8.22	35995
ZmGA2ox8	Zm00001d037724	Zm00001d037724_T003	6	135242721	135243667	+	213	70	10.8	8108.28
ZmGA2ox9	Zm00001d038695	Zm00001d038695_T001	6	162656616	162658442	+	1023	340	8.91	36029.1
ZmGA2ox10	Zm00001d038996	Zm00001d038996_T001	6	168446326	168448293	-	1122	373	8.8	39549.3
ZmGA2ox11	Zm00001d008909	Zm00001d008909_T001	8	24852743	24853978	-	210	69	5.39	7392.33
ZmGA2ox11	Zm00001d008909	Zm00001d008909_T002	8	24852747	24855080	-	1011	336	6.55	35338.2
ZmGA2ox12	Zm00001d012712	Zm00001d012712_T001	8	179086672	179088998	-	1017	338	5.48	35965.7
ZmGA2ox12	Zm00001d012712	Zm00001d012712_T002	8	179086908	179088998	-	1017	338	5.48	35965.7
ZmGA2ox13	Zm00001d024175	Zm00001d024175_T001	10	53027195	53031460	-	1116	371	7.79	39987
ZmGA20ox1	Zm00001d031926	Zm00001d031926_T001	1	206982602	206985131	-	1323	440	5.91	47147.6
ZmGA20ox2	Zm00001d032223	Zm00001d032223_T001	1	217837369	217838681	-	900	299	5.49	32358.7
ZmGA20ox3	Zm00001d034898	Zm00001d034898_T001	1	305074531	305075830	-	1215	404	6.67	45000.9
ZmGA20ox4	Zm00001d003311	Zm00001d003311_T001	2	39752127	39753847	+	903	300	5.32	32327.7
ZmGA20ox5	Zm00001d007894	Zm00001d007894_T001	2	241897454	241898638	-	1185	394	7.26	43119.8
ZmGA20ox6	Zm00001d042611	Zm00001d042611_T001	3	173559174	173562022	-	1161	386	6.52	42510.3
ZmGA20ox7	Zm00001d049926	Zm00001d049926_T001	4	53429242	53431331	-	1212	403	6	43718.8
ZmGA20ox8	Zm00001d052999	Zm00001d052999_T001	4	208285887	208290380	+	1332	443	7.94	49491.7
ZmGA20ox9	Zm00001d013725	Zm00001d013725_T001	5	18631981	18633840	+	1050	349	5.5	39166.3
ZmGA20ox9	Zm00001d013725	Zm00001d013725_T002	5	18632040	18633504	+	1116	371	5.51	40583.7
ZmGA20ox9	Zm00001d013725	Zm00001d013725_T003	5	18632512	18633478	+	585	194	6.36	21546.3
ZmGA20ox9	Zm00001d013725	Zm00001d013725_T004	5	18632631	18633478	+	555	184	5.97	21076.6
ZmGA20ox10	Zm00001d012212	Zm00001d012212_T001	8	170115789	170118570	-	1392	463	8.53	50672.7
ZmGA20ox11	Zm00001d026431	Zm00001d026431_T001	10	145720480	145722209	-	963	320	5.23	35373.9
ZmGA3ox1	Zm00001d039634	Zm00001d039634_T001	3	9745656	9748061	+	1149	382	6.56	41510.5
ZmGA3ox2	Zm00001d037627	Zm00001d037627_T001	6	132317697	132319277	+	1125	374	5.56	41160.1
ZmGA3ox3	Zm00001d018617	Zm00001d018617_T001	7	1105512	1106576	+	1065	354	6.18	39155.2