Literature DB >> 33959914

Feeder-Free Human Embryonic Stem Cell Culture Under Defined Culture Conditions.

Taha Bartu Hayal1, Ayşegül Doğan2.   

Abstract

Human embryonic stem (ES) cell culture has developed over the years allowing the subtle procedures that are easy to manipulate. Feeder-free ES cell culture is an important milestone for human pluripotent stem cell research which eliminates the feeder cells. Various matrices and medium formulations have been generated for feeder-independent culture. Here we described an mTeSR™1 based feeder-independent human ES cell culture on Matrigel® matrix. Culture, freeze/thaw, passaging and initiation of differentiation in suspension culture were described.
© 2021. Springer Science+Business Media, LLC.

Entities:  

Keywords:  Feeder-free culture; Human embryonic stem cells; Induced pluripotent stem cells

Mesh：

Year:  2022        PMID： 33959914     DOI： 10.1007/7651_2021_404

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  1 in total

1.  Snail1-dependent control of embryonic stem cell pluripotency and lineage commitment.

Authors:  Yongshun Lin; Xiao-Yan Li; Amanda L Willis; Chengyu Liu; Guokai Chen; Stephen J Weiss
Journal:  Nat Commun       Date:  2014       Impact factor: 14.919
  1 in total
  1 in total

1.  Surface coating materials regulates the attachment and differentiation of mouse embryonic stem cell derived embryoid bodies into mesoderm at culture conditions.

Authors:  Derya Sağraç; Selinay Şenkal; Taha Bartu Hayal; Fikrettin Şahin; Zehra Çobandede; Ayşegül Doğan
Journal:  Cytotechnology       Date:  2022-02-22       Impact factor: 2.058
  1 in total

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