| Literature DB >> 33954952 |
Andrea Cerrato1, Anna Laura Capriotti1, Carmela Maria Montone2, Sara Elsa Aita1, Giuseppe Cannazza3,4, Cinzia Citti3,4, Susy Piovesana1, Laganà Aldo1,4.
Abstract
The chemical composition of Cannabis sativa L. has been extensively studied for tens of years, but little is known about its lipidome. This chapter describes an analytical workflow for polar lipid determination in hemp. After extraction, lipids are enriched and isolated by graphitized carbon black sorbent, and the isolated lipid is analyzed by liquid chromatography (LC) coupled with high resolution mass spectrometry, leading to identification of many lipid species. We have developed a semi-automated platform using commercially available Lipostar software for lipid identification. Our approach affords the identification of 189 polar lipids in hemp extract, including sulfolipids and phospholipids. The number of the identified lipid species is by far the highest ever reported for Cannabis sativa.Entities:
Keywords: Cannabis sativa; Graphitized carbon black; Lipidomics; Lipostar; Phospholipids; Sulfolipids
Year: 2021 PMID： 33954952 DOI： 10.1007/978-1-0716-1410-5_17
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745