Literature DB >> 33954951

Mass Spectrometric Analysis of Bioactive Sphingolipids in Fungi.

Ashutosh Singh1, Maurizio Del Poeta2,3,4.   

Abstract

As biomolecules, sphingolipids represent a broad spectrum of structures ranging from simple long chain bases to complex glycosphingolipids. While several different mass spectrometry based approaches have been proven to be useful in qualitative and quantitative analysis of sphingolipids, we find that electrospray ionization tandem mass spectrometry (ESI-MS/MS) in the multiple-reaction monitoring (MRM) mode using a triple quadrupole instrument, coupled to high-performance liquid chromatography (HPLC), is the most suitable approach for the analysis. In this chapter, we describe the method in a step-by-step manner towards the targeted analysis of sphingolipids in fungi. With optimized HPLC separation and instrument settings, this MRM approach affords detection of many sphingolipid species simultaneously with good sensitivity.

Entities:  

Keywords:  High-performance liquid chromatography; Mass spectrometry; Multiple-reaction monitoring; Sphingolipids

Year:  2021        PMID： 33954951     DOI： 10.1007/978-1-0716-1410-5_16

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  37 in total

1.  Induction of apoptosis by sphingoid long-chain bases in Aspergillus nidulans.

Authors:  Jijun Cheng; Tae-Sik Park; Li-Chun Chio; Anthony S Fischl; Xiang S Ye
Journal:  Mol Cell Biol       Date:  2003-01       Impact factor: 4.272

2.  Roles for inositol-phosphoryl ceramide synthase 1 (IPC1) in pathogenesis of C. neoformans.

Authors:  C Luberto; D L Toffaletti; E A Wills; S C Tucker; A Casadevall; J R Perfect; Y A Hannun; M Del Poeta
Journal:  Genes Dev       Date:  2001-01-15       Impact factor: 11.361

3.  Sphingoid base synthesis requirement for endocytosis in Saccharomyces cerevisiae.

Authors:  B Zanolari; S Friant; K Funato; C Sütterlin; B J Stevenson; H Riezman
Journal:  EMBO J       Date:  2000-06-15       Impact factor: 11.598

4.  Involvement of yeast sphingolipids in the heat stress response of Saccharomyces cerevisiae.

Authors:  G M Jenkins; A Richards; T Wahl; C Mao; L Obeid; Y Hannun
Journal:  J Biol Chem       Date:  1997-12-19       Impact factor: 5.157

5.  Glucosylceramide synthase is an essential regulator of pathogenicity of Cryptococcus neoformans.

Authors:  Philipp C Rittershaus; Talar B Kechichian; Jeremy C Allegood; Alfred H Merrill; Mirko Hennig; Chiara Luberto; Maurizio Del Poeta
Journal:  J Clin Invest       Date:  2006-06       Impact factor: 14.808

6.  Disruption of the glucosylceramide biosynthetic pathway in Aspergillus nidulans and Aspergillus fumigatus by inhibitors of UDP-Glc:ceramide glucosyltransferase strongly affects spore germination, cell cycle, and hyphal growth.

Authors:  Steven B Levery; Michelle Momany; Rebecca Lindsey; Marcos S Toledo; James A Shayman; Matthew Fuller; Kelly Brooks; Ron Lou Doong; Anita H Straus; Helio K Takahashi
Journal:  FEBS Lett       Date:  2002-08-14       Impact factor: 4.124

Review 7.  Yeast sphingolipids: metabolism and biology.

Authors:  Lina M Obeid; Yasuo Okamoto; Cungui Mao
Journal:  Biochim Biophys Acta       Date:  2002-12-30

8.  Candida albicans sphingolipid C9-methyltransferase is involved in hyphal elongation.

Authors:  Takahiro Oura; Susumu Kajiwara
Journal:  Microbiology       Date:  2009-12-17       Impact factor: 2.777

Review 9.  Sphingolipid functions in Saccharomyces cerevisiae.

Authors:  Robert C Dickson; Robert L Lester
Journal:  Biochim Biophys Acta       Date:  2002-06-13

Review 10.  Synthesis and biological properties of fungal glucosylceramide.

Authors:  Maurizio Del Poeta; Leonardo Nimrichter; Marcio L Rodrigues; Chiara Luberto
Journal:  PLoS Pathog       Date:  2014-01-09       Impact factor: 6.823
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