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Literature DB >> 3395345

Cell-cycle dependent transformation competence in Dictyostelium discoideum.

Abstract

We describe a modification of the transformation procedure for Dictyostelium which allows for a more exact estimate of transformation efficiency and the isolation of primary transformants. Investigations of transformation competence revealed a negative correlation to cell density and a distinct distribution during the cell-cycle. In synchronized cells, transformation efficiency is 2-3 fold higher during mitosis when compared to unsynchronized cells.

Entities: Species

Mesh：

Year: 1988 PMID： 3395345 DOI： 10.1016/0006-291x(88)90648-1

Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575

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Cited

6 in total

1. PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors.

Authors: Hidekazu Kuwayama; Shinji Obara; Takahiro Morio; Mariko Katoh; Hideko Urushihara; Yoshimasa Tanaka
Journal: Nucleic Acids Res Date: 2002-01-15 Impact factor: 16.971

2. Evidence for a feedback regulated back-up promoter which controls permanent expression of a Dictyostelium gene.

Authors: M Maniak; W Nellen
Journal: Nucleic Acids Res Date: 1990-09-25 Impact factor: 16.971

3. Regulation of the Discoidin I gamma gene in Dictyostelium discoideum: identification of individual promoter elements mediating induction of transcription and repression by cyclic AMP.

Authors: F Vauti; P Morandini; J Blusch; A Sachse; W Nellen
Journal: Mol Cell Biol Date: 1990-08 Impact factor: 4.272

Cell-cycle dependent transformation competence in Dictyostelium discoideum.

1. PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors.

2. Evidence for a feedback regulated back-up promoter which controls permanent expression of a Dictyostelium gene.

3. Regulation of the Discoidin I gamma gene in Dictyostelium discoideum: identification of individual promoter elements mediating induction of transcription and repression by cyclic AMP.

4. Identification of a cis-acting element controlling induction of early gene expression in Dictyostelium discoideum.

5. Two separable promoters control different aspects of expression of a Dictyostelium gene.

6. Generation and characterization of Dictyostelium cells deficient in a myosin I heavy chain isoform.