Hai-Anh Ha1,2, Jai-Sing Yang3, Fuu-Jen Tsai4,5,6, Chang-Wei Li7, Yih-Dih Cheng1, Jianyong Li8, Mann-Jen Hour9, Yu-Jen Chiu10,11,12. 1. School of Pharmacy, China Medical University, Taichung, Taiwan, R.O.C. 2. Faculty of Pharmacy, Duy Tan University, Da Nang, Vietnam. 3. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan, R.O.C. 4. Human Genetics Center, Department of Medical Research, Taichung, Taiwan, R.O.C. 5. Department of Medical Genetics, China Medical University Hospital, Taichung, Taiwan, R.O.C. 6. School of Chinese Medicine, China Medical University, Taichung, Taiwan, R.O.C. 7. Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, Taiwan, R.O.C. 8. Army Medical University (The Third Military Medical University), Chongqing, P.R. China. 9. School of Pharmacy, China Medical University, Taichung, Taiwan, R.O.C.; chiou70202@gmail.com mjhou@mail.cmu.edu.tw. 10. Division of Plastic and Reconstructive Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei, Taiwan, R.O.C.; chiou70202@gmail.com mjhou@mail.cmu.edu.tw. 11. Department of Surgery, School of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan, R.O.C. 12. Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan, R.O.C.
Abstract
BACKGROUND/AIM: Glioblastoma multiforme (GBM) is a lethal disease with a high rate of chemoresistance to temozolomide (TMZ). The aim of the study was to establish a TMZ-resistant subline from the GBM-8401 cell line to determine the mechanisms of resistance and identify novel effective therapeutics for TMZ-resistant GBM. MATERIALS AND METHODS: Comparative transcriptome analysis of GBM-8401/TMZR cells and the parental line was performed using Ion Torrent sequencing. Differentially expressed genes (DEGs) between the GBM-8401/TMZR and GBM-8401 cell lines were analyzed. RESULTS: Transcriptomic profiling of GBM-8401/TMZR cells revealed DEGs involved in the retinoblastoma (RB) signaling, DNA damage response (DDR) pathway, and DNA repair mechanisms. CONCLUSION: In vitro and in vivo cell-based GBM models should be used in further biomedical studies to investigate the underlying mechanisms of TMZ-resistant GBM.
BACKGROUND/AIM: Glioblastoma multiforme (GBM) is a lethal disease with a high rate of chemoresistance to temozolomide (TMZ). The aim of the study was to establish a TMZ-resistant subline from the GBM-8401 cell line to determine the mechanisms of resistance and identify novel effective therapeutics for TMZ-resistant GBM. MATERIALS AND METHODS: Comparative transcriptome analysis of GBM-8401/TMZR cells and the parental line was performed using Ion Torrent sequencing. Differentially expressed genes (DEGs) between the GBM-8401/TMZR and GBM-8401 cell lines were analyzed. RESULTS: Transcriptomic profiling of GBM-8401/TMZR cells revealed DEGs involved in the retinoblastoma (RB) signaling, DNA damage response (DDR) pathway, and DNA repair mechanisms. CONCLUSION: In vitro and in vivo cell-based GBM models should be used in further biomedical studies to investigate the underlying mechanisms of TMZ-resistant GBM.