Literature DB >> 3394115

Preparation and characterization of proteolyzed forms of human alpha-thrombin.

P J Braun1, J Hofsteenge, J Y Chang, S R Stone.   

Abstract

The kinetics of the tryptic digestion of human alpha-thrombin were studied. Based on the results of these studies a procedure for the preparation of highly purified, active human beta-thrombin was developed. This beta-thrombin contained less than 5% of other thrombin forms, was active towards tripeptidyl paranitroanilide substrates, but had lost more than 99% of its fibrinogen cleaving activity. Protein-chemical characterization of beta-thrombin showed that it had been cleaved at a single site (Arg73-Asn74) in the beta-chain, in contrast to human beta-thrombin obtained by autolysis, which is cleaved at both Arg-62 and Arg-73.

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Year:  1988        PMID： 3394115     DOI： 10.1016/0049-3848(88)90228-9

Source DB:  PubMed          Journal:  Thromb Res        ISSN： 0049-3848            Impact factor:   3.944


  9 in total

1.  Electrostatic interactions in the association of proteins: an analysis of the thrombin-hirudin complex.

Authors:  A Karshikov; W Bode; A Tulinsky; S R Stone
Journal:  Protein Sci       Date:  1992-06       Impact factor: 6.725

2.  Evidence for common structural changes in thrombin induced by active-site or exosite binding.

Authors:  M A Parry; S R Stone; J Hofsteenge; M P Jackman
Journal:  Biochem J       Date:  1993-03-15       Impact factor: 3.857

3.  An acquired antithrombin autoantibody directed toward the catalytic center of the enzyme.

Authors:  P Sié; A Bezeaud; D Dupouy; G Archipoff; J M Freyssinet; J M Dugoujon; G Serre; M C Guillin; B Boneu
Journal:  J Clin Invest       Date:  1991-07       Impact factor: 14.808

4.  Activation of factor XI by products of prothrombin activation.

Authors:  Anton Matafonov; Suryakala Sarilla; Mao-fu Sun; John P Sheehan; Vladimir Serebrov; Ingrid M Verhamme; David Gailani
Journal:  Blood       Date:  2011-04-28       Impact factor: 22.113

5.  The serine protease granzyme A does not induce platelet aggregation but inhibits responses triggered by thrombin.

Authors:  H S Suidan; K J Clemetson; M Brown-Luedi; S P Niclou; J M Clemetson; J Tschopp; D Monard
Journal:  Biochem J       Date:  1996-05-01       Impact factor: 3.857

6.  Effect of heparin on the glia-derived-nexin-thrombin interaction.

Authors:  A Wallace; G Rovelli; J Hofsteenge; S R Stone
Journal:  Biochem J       Date:  1989-01-01       Impact factor: 3.857

7.  The refined 1.9-A X-ray crystal structure of D-Phe-Pro-Arg chloromethylketone-inhibited human alpha-thrombin: structure analysis, overall structure, electrostatic properties, detailed active-site geometry, and structure-function relationships.

Authors:  W Bode; D Turk; A Karshikov
Journal:  Protein Sci       Date:  1992-04       Impact factor: 6.725

8.  Catalytically competent human and bovine zeta-thrombin and chimeras generated from unfolded polypeptide chains.

Authors:  S D Lewis; D V Brezniak; J W Fenton; J A Shafer
Journal:  Protein Sci       Date:  1992-08       Impact factor: 6.725

9.  The refined 1.9 A crystal structure of human alpha-thrombin: interaction with D-Phe-Pro-Arg chloromethylketone and significance of the Tyr-Pro-Pro-Trp insertion segment.

Authors:  W Bode; I Mayr; U Baumann; R Huber; S R Stone; J Hofsteenge
Journal:  EMBO J       Date:  1989-11       Impact factor: 11.598
  9 in total

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