Solaleh Ghanbarei1,2, Naghmeh Sattarahmady3,4, Farzaneh Zarghampoor5,6, Negar Azarpira7,8, Mahdokht Hossein-Aghdaie2. 1. Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran. 2. Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 3. Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. 4. The Nanobiology and Nanomedicine Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 5. Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. f.zarghampoor@gmail.com. 6. Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Khalili St, Mohamad Rasoolalah Research Tower, 7th floor, Shiraz, Iran. f.zarghampoor@gmail.com. 7. Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. negarazarpira@yahoo.com. 8. Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Khalili St, Mohamad Rasoolalah Research Tower, 7th floor, Shiraz, Iran. negarazarpira@yahoo.com.
Abstract
OBJECTIVE: An attractive cell source for stem cell-based therapy are WJ-MSCs. Hence, tracking WJ-MSCs using non-invasive imaging procedures (such as MRI) and contrast agents (Zn0.5Ni0.5Fe2O4, NFNPs) are required to evaluate cell distribution, migration, and differentiation. RESULTS: Results showed that the bare and dextrin-coated NFNPs were internalized inside the WJ-MSCs and had no effect on the cell viability, proliferation, apoptosis, karyotyping, and morphology of WJ-MSCs up to 125 µg/mL. Besides, treated WJ-MSCs were differentiated into osteo/adipocyte-like cells. The expression of RUNX 2, SPP 1 (P < 0.05), and OCN (P > 0.05) genes in the WJ-MSCs treated with dextrin-coated NFNPs was higher than the untreated WJ-MSCs; and the expression of CFD, LPL, and PPAR-γ genes was reduced in WJ-MSCs treated with both NFNPs in comparison with the untreated WJ-MSCs (P > 0.05). CONCLUSION: Overall, results showed that dextrin-coated NFNPs had no adverse effect on the cellular characteristics, proliferation, and differentiation of WJ-MSCs, and suggesting their potential clinical efficacy.
OBJECTIVE: An attractive cell source for stem cell-based therapy are WJ-MSCs. Hence, tracking WJ-MSCs using non-invasive imaging procedures (such as MRI) and contrast agents (Zn0.5Ni0.5Fe2O4, NFNPs) are required to evaluate cell distribution, migration, and differentiation. RESULTS: Results showed that the bare and dextrin-coated NFNPs were internalized inside the WJ-MSCs and had no effect on the cell viability, proliferation, apoptosis, karyotyping, and morphology of WJ-MSCs up to 125 µg/mL. Besides, treated WJ-MSCs were differentiated into osteo/adipocyte-like cells. The expression of RUNX 2, SPP 1 (P < 0.05), and OCN (P > 0.05) genes in the WJ-MSCs treated with dextrin-coated NFNPs was higher than the untreated WJ-MSCs; and the expression of CFD, LPL, and PPAR-γ genes was reduced in WJ-MSCs treated with both NFNPs in comparison with the untreated WJ-MSCs (P > 0.05). CONCLUSION: Overall, results showed that dextrin-coated NFNPs had no adverse effect on the cellular characteristics, proliferation, and differentiation of WJ-MSCs, and suggesting their potential clinical efficacy.
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