Giovanna Sarra1, Manoel Eduardo de Lima Machado1, Héctor Valentin Caballero-Flores1, Maria Stella Moreira2, Ana Clara Fagundes Pedroni3, Márcia Martins Marques4. 1. Department of Restorative Dentistry, School of Dentistry, University of Sao Paulo (USP), Sao Paulo, SP, Brazil. 2. Post Graduation Program, Ibirapuera University (UNIB), Sao Paulo, SP, Brazil. 3. Department of Restorative Dentistry, School of Dentistry, University of Sao Paulo (USP), Sao Paulo, SP, Brazil; Post Graduation Program, Ibirapuera University (UNIB), Sao Paulo, SP, Brazil. 4. Post Graduation Program, Ibirapuera University (UNIB), Sao Paulo, SP, Brazil. Electronic address: marcia.marques@pq.cnpq.br.
Abstract
BACKGROUND: Dental trauma, restorative operative procedures and/or caries lesions can expose the dental pulp. Facing this clinical condition, where the maintenance of the dentin-pulp complex vitality is imperative, is challenging in Dentistry. Dental pulp stem cells conditioned medium contains trophic factors that could help in this task. This in vivo pilot study aimed to evaluate the effects of the human dental pulp stem cells conditioned medium on the dental pulp tissue response to vital pulp therapy. MATERIAL AND METHODS: Concentrated conditioned medium was obtained by incubating characterized human dental pulp stem cells with fresh culture medium. Pulp exposures performed at the first upper molars (n = 20) of Wistar rats were directly capped with: MTA or MTA + Conditioned Medium. Four and 8 weeks later, the samples were qualitatively analyzed in histological sections (H&E). RESULTS: When the conditioned medium was associated with MTA, there were a high percentage of samples presenting formation of dentin bridges and small percentage of pulp tissue with inflammatory signs in both experimental times. The conditioned medium improved the organization of the newly formed hard tissue. CONCLUSIONS: The association of dental pulp stem cell conditioned medium with MTA showed beneficial effects on dentin-pulp complex regeneration and has promising potential for studies in regenerative dentistry.
BACKGROUND: Dental trauma, restorative operative procedures and/or caries lesions can expose the dental pulp. Facing this clinical condition, where the maintenance of the dentin-pulp complex vitality is imperative, is challenging in Dentistry. Dental pulp stem cells conditioned medium contains trophic factors that could help in this task. This in vivo pilot study aimed to evaluate the effects of the human dental pulp stem cells conditioned medium on the dental pulp tissue response to vital pulp therapy. MATERIAL AND METHODS: Concentrated conditioned medium was obtained by incubating characterized human dental pulp stem cells with fresh culture medium. Pulp exposures performed at the first upper molars (n = 20) of Wistar rats were directly capped with: MTA or MTA + Conditioned Medium. Four and 8 weeks later, the samples were qualitatively analyzed in histological sections (H&E). RESULTS: When the conditioned medium was associated with MTA, there were a high percentage of samples presenting formation of dentin bridges and small percentage of pulp tissue with inflammatory signs in both experimental times. The conditioned medium improved the organization of the newly formed hard tissue. CONCLUSIONS: The association of dental pulp stem cell conditioned medium with MTA showed beneficial effects on dentin-pulp complex regeneration and has promising potential for studies in regenerative dentistry.
Authors: Hisham Elnawam; Menatallah Abdelmougod; Ahmed Mobarak; Mai Hussein; Hamdy Aboualmakarem; Michael Girgis; Rania El Backly Journal: Front Bioeng Biotechnol Date: 2022-02-08