| Literature DB >> 33932339 |
Neil Dani1, Rebecca H Herbst2, Cristin McCabe3, Gilad S Green4, Karol Kaiser5, Joshua P Head1, Jin Cui1, Frederick B Shipley6, Ahram Jang1, Danielle Dionne3, Lan Nguyen3, Christopher Rodman3, Samantha J Riesenfeld3, Jan Prochazka7, Michaela Prochazkova7, Radislav Sedlacek7, Feng Zhang8, Vitezslav Bryja5, Orit Rozenblatt-Rosen3, Naomi Habib9, Aviv Regev10, Maria K Lehtinen11.
Abstract
The choroid plexus (ChP) in each brain ventricle produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier. Here, we construct a single-cell and spatial atlas of each ChP in the developing, adult, and aged mouse brain. We delineate diverse cell types, subtypes, cell states, and expression programs in epithelial and mesenchymal cells across ages and ventricles. In the developing ChP, we predict a common progenitor pool for epithelial and neuronal cells, validated by lineage tracing. Epithelial and fibroblast cells show regionalized expression by ventricle, starting at embryonic stages and persisting with age, with a dramatic transcriptional shift with maturation, and a smaller shift in each aged cell type. With aging, epithelial cells upregulate host-defense programs, and resident macrophages upregulate interleukin-1β (IL-1β) signaling genes. Our atlas reveals cellular diversity, architecture and signaling across ventricles during development, maturation, and aging of the ChP-brain barrier.Entities:
Keywords: aging; brain barrier; cerebrospinal fluid; choroid plexus; development; single-cell RNA sequencing; single-nucleus RNA sequencing
Mesh：
Year: 2021 PMID： 33932339 PMCID： PMC8214809 DOI： 10.1016/j.cell.2021.04.003
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 66.850