| Literature DB >> 3392773 |
A Lorenz1, W Just, M da Silva Cardoso, G Klotz.
Abstract
In contrast to mycoplasma virus L1 and L2 circular DNA, mycoplasma virus L3 linear DNA is not biologically active in polyethylene glycol-mediated transfection. Electroporation of Acholeplasma laidlawii, however, leads to plaque formation after incubation with L3 DNA. The efficiency of electroporation-mediated transfection is 1/10 that of polyethylene glycol-mediated transfection as estimated with L1 DNA. Trypsin treatment of cells before DNA addition increases the efficiency of DNA uptake.Entities:
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Year: 1988 PMID: 3392773 PMCID: PMC253748
Source DB: PubMed Journal: J Virol ISSN: 0022-538X Impact factor: 5.103