| Literature DB >> 33925986 |
Ali Mazloum1, Antoinette van Schalkwyk2, Andrey Shotin1, Alexey Igolkin1, Ivan Shevchenko1, Konstantin N Gruzdev1, Natalia Vlasova1.
Abstract
In this study, we report on the full genome phylogenetic analysis of four ASFV isolates obtained from wild boars in Russia. These samples originated from two eastern and two western regions of Russia in 2019. Phylogenetic analysis indicated that the isolates were assigned to genotype II and grouped according to their geographical origins. The two eastern isolates shared 99.99% sequence identity with isolates from China, Poland, Belgium, and Moldova, whereas the western isolates had 99.98% sequence identity with isolates from Lithuania and the original Georgia 2007 isolate. Based on the full genome phylogenies, we identified three single locus targets, MGF-360-10L, MGF-505-9R, and I267L, that yielded the same resolving power as the full genomes. The ease of alignment and a high level of variation make these targets a suitable selection as additional molecular markers in future ASFV phylogenetic practices.Entities:
Keywords: African swine fever virus; Russian isolates; full-genome sequence; genome markers; phylogenetic analysis
Year: 2021 PMID: 33925986 DOI: 10.3390/pathogens10050521
Source DB: PubMed Journal: Pathogens ISSN: 2076-0817