Giuseppe Broggi1, Lucia Salvatorelli1, Davide Barbagallo2, Francesco Certo3, Roberto Altieri3, Elena Tirrò4, Michele Massimino4, Paolo Vigneri4, Elia Guadagno5, Grazia Maugeri6, Velia D'Agata6, Giuseppe Musumeci6, Marco Ragusa2, Giuseppe Maria Vincenzo Barbagallo3, Daniela Russo5, Rosario Caltabiano1. 1. Department of Medical and Surgical Sciences and Advanced Technologies "G.F. Ingrassia", Anatomic Pathology, University of Catania, 95123 Catania, Italy. 2. Department of Biomedical and Biotechnological Sciences, Section of Biology and Genetics Giovanni Sichel, University of Catania, 95123 Catania, Italy. 3. Department of Medical and Surgical Sciences and Advanced Technologies "G.F. Ingrassia", Neurological Surgery, Policlinico "G. Rodolico-San Marco" University Hospital, University of Catania, 95123 Catania, Italy. 4. Department of Clinical and Experimental Medicine, Center of Experimental Oncology and Hematology, Policlinico "G. Rodolico-San Marco" University Hospital, University of Catania, 95123 Catania, Italy. 5. Department of Advanced Biomedical Sciences, Pathology Section, University of Naples Federico II, 80131 Naples, Italy. 6. Department of Biomedical and Biotechnological Sciences, Section of Anatomy, Histology and Movement Sciences, University of Catania, 95123 Catania, Italy.
Abstract
BACKGROUND: The aim of this study was to investigate the immunohistochemical expression and distribution of serine and arginine rich splicing factor 1 (SRSF1) in a series of 102 cases of both diffuse and circumscribed adult gliomas to establish the potential diagnostic role of this protein in the differential diagnosis of brain tumors. METHODS: This retrospective immunohistochemical study included 42 glioblastoma cases, 21 oligodendrogliomas, 15 ependymomas, 15 pilocytic astrocytomas, 5 sub-ependymal giant cell astrocytoma and 4 pleomorphic xanthoastrocytomas. RESULTS: Most glioblastoma (81%), oligodendroglioma (71%), sub-ependymal giant cell astrocytoma (80%) and pleomorphic xanthoastrocytoma (75%) cases showed strong SRSF1 immunoexpression, while no detectable staining was found in the majority of ependymomas (87% of cases) and pilocytic astrocytomas (67% of cases). CONCLUSIONS: The immunohistochemical expression of SRSF1 may be a promising diagnostic marker of astrocytomas and oligodendrogliomas and its increased expression might allow for excluding entities that often enter into differential diagnosis, such as ependymomas and pilocytic astrocytomas.
BACKGROUND: The aim of this study was to investigate the immunohistochemical expression and distribution of serine and arginine rich splicing factor 1 (SRSF1) in a series of 102 cases of both diffuse and circumscribed adult gliomas to establish the potential diagnostic role of this protein in the differential diagnosis of brain tumors. METHODS: This retrospective immunohistochemical study included 42 glioblastoma cases, 21 oligodendrogliomas, 15 ependymomas, 15 pilocytic astrocytomas, 5 sub-ependymal giant cell astrocytoma and 4 pleomorphic xanthoastrocytomas. RESULTS: Most glioblastoma (81%), oligodendroglioma (71%), sub-ependymal giant cell astrocytoma (80%) and pleomorphic xanthoastrocytoma (75%) cases showed strong SRSF1 immunoexpression, while no detectable staining was found in the majority of ependymomas (87% of cases) and pilocytic astrocytomas (67% of cases). CONCLUSIONS: The immunohistochemical expression of SRSF1 may be a promising diagnostic marker of astrocytomas and oligodendrogliomas and its increased expression might allow for excluding entities that often enter into differential diagnosis, such as ependymomas and pilocytic astrocytomas.
Authors: David N Louis; Arie Perry; Guido Reifenberger; Andreas von Deimling; Dominique Figarella-Branger; Webster K Cavenee; Hiroko Ohgaki; Otmar D Wiestler; Paul Kleihues; David W Ellison Journal: Acta Neuropathol Date: 2016-05-09 Impact factor: 17.088
Authors: Roberto Altieri; Antonio Morrone; Francesco Certo; Giuseppe Parisi; Giovanni Buscema; Giuseppe Broggi; Gaetano Magro; Giuseppe M Barbagallo Journal: World Neurosurg Date: 2019-07-23 Impact factor: 2.104
Authors: David E Reuss; Felix Sahm; Daniel Schrimpf; Benedikt Wiestler; David Capper; Christian Koelsche; Leonille Schweizer; Andrey Korshunov; David T W Jones; Volker Hovestadt; Michel Mittelbronn; Jens Schittenhelm; Christel Herold-Mende; Andreas Unterberg; Michael Platten; Michael Weller; Wolfgang Wick; Stefan M Pfister; Andreas von Deimling Journal: Acta Neuropathol Date: 2014-11-27 Impact factor: 17.088
Authors: Mélanie Pagès; Kristian W Pajtler; Stéphanie Puget; David Castel; Nathalie Boddaert; Arnault Tauziède-Espariat; Stéphanie Picot; Marie-Anne Debily; Marcel Kool; David Capper; Christian Sainte-Rose; Fabrice Chrétien; Stefan M Pfister; Torsten Pietsch; Jacques Grill; Pascale Varlet; Felipe Andreiuolo Journal: Brain Pathol Date: 2018-11-28 Impact factor: 6.508
Authors: Giuseppe Broggi; Francesco Certo; Roberto Altieri; Rosario Caltabiano; Marco Gessi; Giuseppe Maria Vincenzo Barbagallo Journal: Surg Neurol Int Date: 2021-09-20
Authors: Giuseppe Broggi; Eliana Piombino; Roberto Altieri; Chiara Romano; Francesco Certo; Giuseppe Maria Vincenzo Barbagallo; Paolo Vigneri; Dario Condorelli; Lorenzo Colarossi; Cristina Colarossi; Gaetano Magro; Elena Tirrò Journal: Front Neurol Date: 2022-02-09 Impact factor: 4.003
Authors: Giuseppe Broggi; Giuseppe Angelico; Veronica Filetti; Caterina Ledda; Claudia Lombardo; Ermanno Vitale; Venerando Rapisarda; Carla Loreto; Rosario Caltabiano Journal: Int J Environ Res Public Health Date: 2021-06-09 Impact factor: 3.390