Simona Mattioli1, Giulia Collodel2, Cinzia Signorini2, Elisa Cotozzolo1, Daria Noto2, Daniela Cerretani3, Lucia Micheli3, Anna Ida Fiaschi3, Gabriele Brecchia4, Laura Menchetti5, Elena Moretti2, Camille Oger6, Claudio De Felice7, Cesare Castellini1. 1. Department of Agricultural, Environmental and Food Science, University of Perugia, Borgo 20 Giugno, 74, 06123 Perugia, Italy. 2. Department of Molecular and Developmental Medicine, University of Siena, Policlinico Santa Maria alle Scotte, Viale Bracci 16, 53100 Siena, Italy. 3. Department of Medicine, Surgery and Neurosciences, University of Siena, Policlinico Santa Maria alle Scotte, Viale Bracci, 16, 53100 Siena, Italy. 4. Department of Veterinary Medicine, University of Milan, Via dell'Università 6, 26900 Lodi, Italy. 5. Department of Agricultural and Agri-Food Sciences and Technologies, University of Bologna, Viale Fanin 46, 40138 Bologna, Italy. 6. Institut des Biomolécules Max Mousseron (IBMM), UMR, CNRS, Université de Montpellier, ENSCM, 5247 Montpellier, France. 7. Neonatal Intensive Care Unit, Azienda Ospedaliera Universitaria Senese, 53100 Siena, Italy.
Abstract
Polyunsaturated fatty acid (PUFA) metabolism and tissue distribution is modulated by the oxidation of these molecules. This research aimed to investigate the implication of dietary n-3 PUFA supplementation (precursor and long-chain PUFA) on the PUFA profile and oxidative status of the liver, testis, and brain of adult rabbit bucks. Twenty New Zealand White rabbit bucks were divided into four experimental groups (n = 5 per group) and were fed different diets for 110 days: control (CNT), standard diet containing 50 mg/kg alpha-tocopheryl acetate (vitamin E); CNT+, standard diet + 200 mg/kg vitamin E; FLAX, standard diet + 10% flaxseed + 200 mg/kg vitamin E; or FISH, standard diet + 3.5% fish oil + 200 mg/kg vitamin E. Antioxidants (enzymatic and non-enzymatic), oxidative status (malondialdehyde and isoprostanoids), and n-3 and n-6 PUFAs of tissues were analysed. A chain mechanism of oxidant/antioxidant molecules, which largely depended on the particular PUFA composition, was delineated in the different organs. The liver showed an oxidant/antioxidant profile and lipid pathways widely modulated by PUFA and vitamin E administration; on the other hand, the testis' oxidative profile rather than its lipid profile seemed to be particularly affected, an outcome opposite to that of the brain (modulation operated by dietary PUFA).
Polyunsaturated fatty acid (n class="Chemical">PUFA) metabolism and tissue distribution is modulated by the oxidation of these molecules. This research aimed to investigate the implication of dietary n-3 PUFA supplementation (precursor and long-chain PUFA) on the PUFA profile and oxidative status of the liver, testis, and brain of adult rabbit bucks. Twenty New Zealand White rabbit bucks were divided into four experimental groups (n = 5 per group) and were fed different diets for 110 days: control (CNT), standard diet containing 50 mg/kg alpha-tocopheryl acetate (vitamin E); CNT+, standard diet + 200 mg/kg vitamin E; FLAX, standard diet + 10% flaxseed + 200 mg/kg vitamin E; or FISH, standard diet + 3.5% fish oil + 200 mg/kg vitamin E. Antioxidants (enzymatic and non-enzymatic), oxidative status (malondialdehyde and isoprostanoids), and n-3 and n-6PUFAs of tissues were analysed. A chain mechanism of oxidant/antioxidant molecules, which largely depended on the particular PUFA composition, was delineated in the different organs. The liver showed an oxidant/antioxidant profile and lipid pathways widely modulated by PUFA and vitamin E administration; on the other hand, the testis' oxidative profile rather than its lipid profile seemed to be particularly affected, an outcome opposite to that of the brain (modulation operated by dietary PUFA).
Authors: A Dal Bosco; Zs Gerencsér; Zs Szendrő; C Mugnai; M Cullere; M Kovàcs; S Ruggeri; S Mattioli; C Castellini; A Dalle Zotte Journal: Meat Sci Date: 2013-06-15 Impact factor: 5.209
Authors: Stella Agradi; Susanna Draghi; Elisa Cotozzolo; Olimpia Barbato; Marta Castrica; Alda Quattrone; Majlind Sulce; Daniele Vigo; Laura Menchetti; Maria Rachele Ceccarini; Egon Andoni; Federica Riva; Maria Laura Marongiu; Giulio Curone; Gabriele Brecchia Journal: Front Vet Sci Date: 2022-01-31