Didem Ag Seleci1,2, Viktor Maurer1,2, Firat Baris Barlas3, Julian Cedric Porsiel1, Bilal Temel1, Elcin Ceylan3, Suna Timur3, Frank Stahl4, Thomas Scheper4, Georg Garnweitner1,2. 1. Institute for Particle Technology (iPAT), Technische Universität Braunschweig, 38104 Braunschweig, Germany. 2. Center of Pharmaceutical Engineering (PVZ), Technische Universität Braunschweig, 38106 Braunschweig, Germany. 3. Faculty of Science Biochemistry Department, Ege University, 35100 Izmir, Turkey. 4. Institute for Technical Chemistry, Leibniz University Hannover, 30167 Hannover, Germany.
Abstract
The development of multifunctional nanoscale systems that can mediate efficient tumor targeting, together with high cellular internalization, is crucial for the diagnosis of glioma. The combination of imaging agents into one platform provides dual imaging and allows further surface modification with targeting ligands for specific glioma detection. Herein, transferrin (Tf)-decorated niosomes with integrated magnetic iron oxide nanoparticles (MIONs) and quantum dots (QDs) were formulated (PEGNIO/QDs/MIONs/Tf) for efficient imaging of glioma, supported by magnetic and active targeting. Transmission electron microscopy confirmed the complete co-encapsulation of MIONs and QDs in the niosomes. Flow cytometry analysis demonstrated enhanced cellular uptake of the niosomal formulation by glioma cells. In vitro imaging studies showed that PEGNIO/QDs/MIONs/Tf produces an obvious negative-contrast enhancement effect on glioma cells by magnetic resonance imaging (MRI) and also improved fluorescence intensity under fluorescence microscopy. This novel platform represents the first niosome-based system which combines magnetic nanoparticles and QDs, and has application potential in dual-targeted imaging of glioma.
The development of multifunctional nanoscale systems that can mediate efficient tumor targeting, together with high cellular internalization, is crucial for the diagnosis of pan class="Disease">glioma. The combination of imaging agents into one platform provides dual imaging and allows further surface modification with targeting ligands for specific glioma detection. Herein, transferrin (Tf)-decorated niosomes with integrated magnetic iron oxide nanoparticles (MIONs) and quantum dots (QDs) were formulated (PEGNIO/QDs/MIONs/Tf) for efficient imaging of glioma, supported by magnetic and active targeting. Transmission electron microscopy confirmed the complete co-encapsulation of MIONs and QDs in the niosomes. Flow cytometry analysis demonstrated enhanced cellular uptake of the niosomal formulation by glioma cells. In vitro imaging studies showed that PEGNIO/QDs/MIONs/Tf produces an obvious negative-contrast enhancement effect on glioma cells by magnetic resonance imaging (MRI) and also improved fluorescence intensity under fluorescence microscopy. This novel platform represents the first niosome-based system which combines magnetic nanoparticles and QDs, and has application potential in dual-targeted imaging of glioma.
Entities:
Keywords:
glioma imaging; iron oxide nanoparticles; multifunctional niosomes; quantum dots
Authors: Didem Ag; Muharrem Seleci; Rebecca Bongartz; Mustafa Can; Seda Yurteri; Ioan Cianga; Frank Stahl; Suna Timur; Thomas Scheper; Yusuf Yagci Journal: Biomacromolecules Date: 2013-09-12 Impact factor: 6.988
Authors: Maria João Ramalho; Joana Angélica Loureiro; Manuel A N Coelho; Maria Carmo Pereira Journal: Pharmaceutics Date: 2022-01-25 Impact factor: 6.321