Impact of Fertilizer on Crop Yield and C:N:P Stoichiometry in Arid and Semi-Arid Soil.

Fertilization can significantly affect the quality of crop and soil. To determine the effects of long-term fertilization on crop yield and carbon:nitrogen:phosphorus (C:N:P) stoichiometry in soil, a study was conducted on the terraced fields of the Loess Plateau from 2007 to 2019. Nine fertilization treatments were included: no fertilizer; organic fertilizer (O); organic and nitrogen fertilizers (ON); organic, nitrogen, and phosphorus fertilizers (ONP); organic and phosphorus fertilizers (OP); phosphorus and nitrogen fertilizers; potash and nitrogen fertilizers; potash, nitrogen, and phosphorus fertilizers; and potash and phosphorus fertilizers. Under these treatments except for CK and PK, crop yields initially decreased but later increased. The nutrient content and C:N:P stoichiometry increased in soil depth of 0-20 cm. The soil available nutrients did not change significantly with the duration of fertilization. The O, ON, ONP, and OP had the most evident effect on the enhancement of soil nutrient content, whereas O and ON had the most evident effect on the increase in soil organic carbon (SOC):total phosphorus (TP) and total nitrogen (TN):TP. In soil depth of 0-20 cm, crop yield, SOC:TN, SOC:TN, SOC:TP, and TN:TP significantly correlated with soil nutrients. This study indicated that long-term fertilization can effectively improve crop yield, soil fertility, and soil C:N:P stoichiometry. Meanwhile, the single application of an organic fertilizer or the combination of organic and nitrogen fertilizers can improve the condition of nitrogen limitation in arid and semi-arid areas.

1. Introduction

Ecological stoichiometry is the science of studying the energy balpan class="Chemical">ance of biological systems pan class="Chemical">and multiple nutrient elements (mainly, ppan class="Chemical">an class="Chemical">carbon (C), anpan> class="Chemical">nitrogen (N), and phosphorus (P)) [1]. It provides a new comprehensive method for studying plant–soil interactions and C, N, and P cycles [1,2]. Cropland ecosystems are one of the most frequently disturbed ecosystems by human activities. Soil is an important part of the ecosystem and the basis for plant growth. C, N, P, and other nutrient elements are important factors for plant growth and development. They directly affect plant growth and development, soil microbial activities, and soil nutrient cycling [3].

In agricultural production practices, fertilizers (organic n>an class="Chemical">and inorganic) are used to increase the accumulation of C, N, and P in the soil to improve the soil’s ability to supply nutrients to crops [4]. Fertilizers have considerably contributed to increase in food production and food security [5,6,7]. However, there are problems with high frequency of fertilizer application and low utilization efficiency [8,9]. The use of large amounts of chemical fertilizers has caused a series of harmful ecological and environmental impacts, such as soil acidification, soil compaction, and soil fertility degradation, which severely restrict the sustainable development of green agriculture [9,10]. Therefore, rationally balancing chemical fertilizer input and enhancing crop yields and soil sustainability are the targets that need to be focused urgently in current agricultural production process.

Fertilization is one of the most important methods in agricultural production. Studies have found that soil nutrients and crop growth are affected by frequent fertilization and other factors [9,11]. Fertilization leads to changes in crop yield and soil nutrient content [6,12]. Luo et al. (2020) [13] reported that fertilization significantly increased the organic and activated an class="Chemical">carbon contents in soil but reduced the soil organpan>ic an class="Chemical">carbon (SOC):total nitrogen (TN) and SOC:total phosphorus (TP) ratios; however, there were considerable differences among different fertilization methods. A study has reported that excessive fertilization reduces the overall soil quality [13]. Additionally, a study has reported that fertilization affects soil nutrient content and crop yield by affecting the ability of crops to absorb and distribute elements, such as N and P [12]. Studying the changes in characteristics of soil C, N, and P and their stoichiometric ratios under long-term fertilization conditions can help us understand in detail the status of soil nutrient cycling during plant growth, and the use of resources by plants during fertilization [14,15]. At the same time, studying the changes in soil stoichiometry under long-term fertilization can help us understand nutrient cycling and nutrient limitation in the soil ecosystem [16]. It is of great significance to understand the sustainability of soil quality and crop yield after long-term fertilization.

In the present study, we chose a typical terraced field plot in the Loess Plateau to conduct a 12-year experiment (from 2007 to 2019) on various fertilization treatments. The objective was to study the changes in characteristics of cropn> yield, soil nutrient content, n>an class="Chemical">and C:N:P stoichiometry with various fertilization methods under different fertilization durations. We hypothesized that (1) long-term fertilization may affect the crop yield and soil nutrient content, (2) the changes in soil nutrient content may alleviate the C:N:P stoichiometry of soil, and (3) the single application of an organic fertilizer or an organic fertilizer combined with an inorganic fertilizer may have an advantage in increasing crop yield and soil nutrients supply.

2. Materials and Methods

2.1. Study Area

The study site was located at the ppan class="Chemical">an class="Chemical">Anclass="Chemical">n>an>sai Research Station of the Chinese Academy of Sciences (36°51 N, 109°19 E, 1068–1309 m above sea level), which is a long-term detection sampling site (Figure 1). The anpan>nual average temperature is 8.8 °C. The anpan>nual average rainfall is 500 mm, which is mainly observed in July–September. It is a typical hilly and gully area in the Loess Plateau, an ecologically fragile area in the northwest China, and a key area for returning farmland to forest (grass) [17]. The soil type is loess soil.

Figure 1

The locations of study sites in Ansai County, Shanxi Province, China.

2.2. Field Experiment and Design

In the experiment, a rectpan class="Chemical">angular block with pan class="Chemical">an area of 1080 m2 was selected and divided into 36 small plots of 3.5 m × 8.57 m. In total, 9 fertilizer treatments were performed, and each treatment was repeated 4 times (Table 1). The an class="Disease">crop rotation mode was an class="Species">millet → glutinous millet → millet → soybean. The crops were planted once a year without irrigation. In 2007, millet was planted first. For unification, this study was for only selected crops and soil that were planted for millet. The basic soil physical and chemical properties before sowing are shown in Table S1.

Table 1

Experimental design of the different fertilization treatments.

Experimental Plots
1ON	2O	3NPK	4PK	5NK	6NP	7CK	8PNK	9PP
10OP	11ON	12PK	13NK	14CK	15NPK	16NK	17O	18ONP
19ONP	20OP	21CK	22NK	23NP	24PK	25NPK	26ON	27O
28O	29ONP	30NP	31CK	32NPK	33NK	34PK	35OP	36ON

Fertilization included nine treatments, CK, O, ON, ONP, OP, NP, NK, NPK, and PK, the details of which cn>an class="Chemical">an be found in Table 2. Winter an class="Species">sheep manpan>ure was selected as the organn>an>ic fertilizer, and an class="Chemical">urea, an class="Chemical">diammonium phosphate, and n class="Chemical">potassium sulfate were selected as nitrogen, phosphorus, and potash fertilizers, respectively.

Table 2

Experimental fertilization.

Treatment	Illustration
CK	No fertilizer
O	0.75 kg/m2 organic fertilizer
ON	0.75 kg/m2 organic fertilizer and 0.021 kg/m2 nitrogen
ONP	0.75 kg/m2 organic fertilizer, 0.021 kg/m2 nitrogen and 0.017 kg/m2 phosphorus
OP	0.75 kg/m2 organic fertilizer and 0.017 kg/m2 phosphorus
NP	0.017 kg/m2 phosphorus and 0.021 kg/m2 nitrogen
NK	0.012 kg/m2 potash and 0.021 kg/m2 nitrogen
NPK	0.012 kg/m2 potash, 0.021 kg/m2 nitrogen and 0.017 kg/m2 phosphorus
PK	0.012 kg/m2 potash and 0.017 kg/m2 phosphorus

Sowing was performed in May every year. Before sowing the crop seeds, all plots were irrigated to meet the soil moisture requirement for crop growth. Two days after irrigation, fertilizer was applied, the artificial soil and fertilizer were mixed evenly, n>an class="Chemical">and the crop seeds were sown. Irrigation was not performed during the crop growth phase, and when the crops were mature, they were harvested by artificial harvesting. After harvesting, the soil was plowed manually to prepare it for crop planting later.

2.3. Soil Sampling and Analysis

We collected soil samples from each plot according to the S-type sampling method [18]. The soil depths for collection were 0–20 pan class="Chemical">and 20–40 cm. Before collecting the sampn>les, the litter on the surface ground was removed. Furthermore, the soil sampn>les from each plot were mixed evenly as one soil sampn>le pan class="Chemical">and were brought back to the laboratory [18].

First, small stones were removed from the soil samples, and the roots and small animals were visible. Furthermore, it was passed through a 2-mm sieve and air-dried naturally for the determination of soil nutrient content and pH. SOC and soil TN were determined using the an class="Chemical">H2SO4–K2an class="Species">Cr2O7 method [19] and Kjeldahl method [19], respectively. Soil TP was determined using the molybdenum blue method [18]. Soil available nitrogen (AN) was determined using the alkaline KMnO4 method [19], and available phosphorus (AP) was determined using the Olsen method [19].

2.4. Statistical Analysis

Effects of the type of fertilization treatment pan class="Chemical">and duration of fertilization on crop yield, SOC, pan class="Chemical">TN, pan class="Chemical">TP, an class="Chemical">AN, AP, pH, SOC:TN, SOC:TP, TN:TP, and anpan> class="Chemical">AN:AP were evaluated using two-way ANOVA. One-way ANOVA was used to evaluate the crop yield, SOC, TN, TP, AN, AP, pH, SOC:TN, SOC:TP, TN:TP, and AN:AP responses to the duration of fertilization. Duncan’s post hoc test at P < 0.05 was used for multiple comparisons. Before ANOVA, one sample Kolmogorov–Smirnov test and the homogeneity of variance test were performed to determine whether the parameters were normally distributed and the variances were homogeneous. If not, a logarithmic transformation was used for the corresponding parameters. A correlation matrix was used to study the correlations among crop yield, SOC, TN, TP, AN, AP, pH, SOC:TN, SOC:TP, TN:TP, and AN:AP in soil depths of 0–20 and 20–40 cm during the plantation of three succession species. The correlation matrix was visualized using R. 4.0.2 (corrplot) software.

3. Results

3.1. Crop Yield

The type of fertilization treatment pan class="Chemical">and duration of fertilization had a considerable impact on crop yield (Table 3). Between 2007 n>an class="Chemical">and 2019, crop yield after the nine fertilization treatments initially decreased but later increased as the number of years of fertilization increased (Figure 2). For OPN, NPK, ON, NP, OP, and O treatments, the crop yield was the highest in 2019.

Table 3

F-value and P-value of year, fertilizer treatments (FT), and their interactions with various parameters studied by a two-way ANOVA.

	Indexes	Year		FT		Year × FT
		F	P	F	P	F	P
	Yield	279.13	0.00	40.49	0.00	10.29	0.00
0–20 cm	SOC	16.73	0.00	137.24	0.00	1.14	0.34
	TN	11.07	0.00	185.63	0.00	1.24	0.25
	TP	10.53	0.00	149.99	0.00	0.51	0.98
	AN	0.39	0.81	39.33	0.00	0.85	0.68
	AP	6.93	0.00	64.29	0.00	2.30	0.00
	pH	8.41	0.00	2.80	0.01	0.96	0.54
	SOC:TN	37.90	0.00	5.97	0.00	2.04	0.01
	SOC:TP	8.22	0.00	296.55	0.00	1.63	0.07
	TN:TP	2.69	0.04	361.38	0.00	1.67	0.06
	AN:AP	1.38	0.26	13.55	0.00	0.66	0.89
20–40 cm	SOC	3.13	0.02	5.47	0.00	0.92	0.60
	TN	10.84	0.00	4.41	0.00	1.14	0.34
	TP	2.74	0.04	6.64	0.00	0.61	0.93
	AN	9.31	0.00	2.77	0.01	0.94	0.57
	AP	2.87	0.03	5.61	0.00	1.15	0.33
	pH	8.99	0.00	3.51	0.00	0.55	0.96
	SOC:TN	43.09	0.00	1.83	0.10	1.36	0.17
	SOC:TP	1.72	0.16	12.41	0.00	1.23	0.26
	TN:TP	14.57	0.00	9.69	0.00	1.10	0.38
	AN:AP	4.69	0.00	4.74	0.00	0.98	0.52

Note: SOC, soil organic carbon; TN, total nitrogen; TP, total phosphorus; AN, available nitrogen; AP, available phosphorus.

Figure 2

Crop yield after various fertilization treatments. Values are presented as the mean ± SE.

3.2. Soil Nutrients

At 0–20 cm soil depths, the duration of fertilization had a significpan class="Chemical">ant impact on SOC, pan class="Chemical">TN, pan class="Chemical">TP, AP, and pH (Table 3). Between 2007 and 2019, SOC, TN, and TP after all the fertilization treatments exhibited an overall increasing trend with the duration of fertilization (Figure 3a–c). The an class="Chemical">AN and AP showed no marked changes with the duration of fertilization, whereas the overall pH decreased (Figure 3d–f).

Figure 3

Effects on soil nutrients [SOC (a), TN (b), TP (c), AN (d), and AP (e)] and pH (f) after various fertilization treatments in soil depths of 0–20 cm. Notes: Values are presented as the mean ± SE; Soil samples used for AP and AN determination of 2009 and 2011 are missing.

In soil depths of 20–40 cm, the duration of fertilization had a significpan class="Chemical">ant impact on SOC, pan class="Chemical">TN, pan class="Chemical">TP, an class="Chemical">AN, AP, and pH (Table 3). Between 2007 anpan>d 2019, the SOC initially increased ann>an>d further stabilized as the duration of fertilization increased (Figure 4a–c), whereas TN, TP, n class="Chemical">AN, AP, and pH did not exhibit evident changes (Figure 4d–f).

Figure 4

Effects on soil nutrients (SOC (a), TN (b), TP (c), AN (d), and AP (e)) and pH (f) after various fertilization treatments in soil depths of 20–40 cm. Notes: Values are presented as the mean ± SE; Soil samples used for AP and AN determination of 2009 and 2011 are missing.

3.3. Soil C:N:P Stoichiometry

In soil depths of 0–20 cm, the duration of fertilization had a significpan class="Chemical">ant impact on SOC:pan class="Chemical">TN, SOC:pan class="Chemical">TP, TN:TP, and an class="Chemical">AN:AP (Table 3). Between 2007 and 2019, SOC:TN and SOC:TP after all the fertilization treatments and TN:TP after O and ON treatments exhibited anpan> overall increasing trend with the duration of fertilization (Figure 4a,b), whereas ann>an> class="Chemical">AN:AP did not exhibit any evident changes (Figure 5d).

Figure 5

C:N:P stoichiometry (SOC:TN (a), SOC:TP (b), TN:TP (c), and AN:AP (d)) in soil after various fertilization treatments in soil depths of 0–20 cm. Notes: Values are presented as the mean ± SE; Soil samples used for AP and AN determination of 2009 and 2011 are missing.

In soil depths of 20–40 cm, the duration of fertilization had a significpan class="Chemical">ant impact on SOC:pan class="Chemical">TN, SOC:pan class="Chemical">TP, TN:TP, and an class="Chemical">AN:AP (Table 3). Between 2007 and 2019, SOC:TN after all the fertilization treatments exhibited an overall increasing trend with the duration of fertilization (Figure 6a); the SOC:TP initially increased and later stabilized (Figure 6b), whereas TN:TP and anpan> class="Chemical">AN:AP did not exhibit any evident changes (Figure 6c,d).

Figure 6

C:N:P stoichiometry (SOC:TN (a), SOC:TP (b), TN:TP (c), and AN:AP (d)) in soil after various fertilization treatments in soil depths of 20–40 cm. Notes: Values are presented as the mean ± SE; Soil samples used for AP and AN determination of 2009 and 2011 are missing.

3.4. The Relationship among Various Parameters

For the soil depth of 0–20 cm, the correlation matrix showed that crop yield was significantly correlated with SOC, n>an class="Chemical">TN, TP, AN, AP, pH, and SOC:TN; SOC:TN with SOC, TN, AN, AP, pH, SOC:TP, and TN:TP; SOC:TP with SOC, TN, TP, AN, AP, TN:TP, and AN:AP; and TN:TP with SOC, TN, TP, AN, AP, pH, and AN:AP (Figure 7a).

Figure 7

Correlation matrix among the different parameters determined for soil depths of 0–20 cm (a) and 20–40 cm (b). Notes: ×, correlation is non-significant at P < 0.05.

For the soil depth of 20–40 cm, the correlation matrix showed that crop yield was significantly correlated with SOC and TP; SOC:TN with SOC, TN, AN, AP, pH, and SOC:TP; SOC:TP with SOC, TN, TP, and TN:TP; and TN:TP with SOC, TN, TP, AN, pH, and AN:AP (Figure 7b).

4. Discussion

4.1. Long-Term Fertilization Altered Crop Yield

Increasing crop production is one of the ways to increase agricultural development in arid and semi-arid regions. Fertilization is n>an class="Chemical">an important agronomic measure, and appropriate application of fertilizers can promote the growth and development of farming and improve the quality and yield of crops [20,21]. This study demonstrated that after the application of organic fertilizers, inorganic fertilizers, or a combination of both, crop yield initially decreased but later increased significantly as the duration of fertilization increased.

This result cpan class="Chemical">an be explained as follows. First, N, n>n>an class="Chemical">an class="Chemical">P, and potassium (K) are essential nutrients for the growth ann>an>d development of crops. During the long-term growth of crops, the nutrient elements in the soil are consumed in large quantities. The addition of artificial fertilizers compensates for the deficiency of soil nutrients, thereby increasing the absorption and assimilation of soil N, P, and K by plants; this affects the growth and development of crops, further affecting crop yield [22]. Second, with the extension of the duration of fertilization, the application of fertilizer gradually increases the basic soil fertility; the correlation matrix showed that the crop yield and soil nutrient content were positively correlated. When it canpan> provide enough mineral nutrients, organpan>ic matter gradually releases nutrients through mineralization to increase crop yields. The decrease in yield in the initial stage of fertilization may be because of the effect of local climate conditions or factors such as plant growth.

Studies have reported that organic fertilizer is released slowly, and it cannot meet the growth needs of crops in the current season. However, the fertilizer effect is long [21,23]. This study demonstrated that the combination of organic and inorganic fertilizers or the combination of inorganic fertilizers have clear yield-enhancing effects. The results of this study showed that fertilization is one of the key technical measures to maintain sustainable crop productivity. A reasonable combination of fertilizers is more conducive to fertilize the soil and provide conditions for the full growth of crops. Gosal et al. (2018) [10] reported that the combined application of inorganic and organic fertilizers or mixture of inorganic fertilizers doubled the input of N, P, and K; significantly increased the nutrient content in the soil; and helped in meeting the nutrient requirement for crop growth. This resulted in a considerable increase in crop yield. The long-term addition of fertilizers can release fixed elements in soil, improve soil structure and ecological conditions, and balance the soil’s capacity of an class="Chemical">water and fertilizer supply [9]. On the contrary, long-term planting without fertilization leads to continuous nutrient deficiency in the soil, the soil becomes increasingly barren, and the nutrients will not suffice for the growth of the crop, which affects the crop yield.

4.2. Long-Term Fertilization Altered Soil Nutrients

Soil nutrients cpan class="Chemical">an provide the material basis n>an class="Chemical">and energy source for the survival of various microorganisms in the soil and the growth of crops [24]. Soil nutrients reflect the maturity of the soil to a certain extent [25]. This study reported that the SOC, TN, and TP content of the surface layer (0–20 cm) of soil after fertilization exhibited an overall increasing trend as the duration of fertilization increased. The increase in soil nutrients is mainly due to the input of exogenous soil nutrients. Moreover, soil microbial activity determines the intensity of the soil biochemical reaction processes and participates in the circulation and transformation of nutrients in the soil, which can reflect the status of soil fertility [26]. Studies have reported that fertilization can increase the diversity and composition of soil microorganisms, thereby producing a large number of enzymes [6,27] and improving soil fertility in turn. In addition, soil pH has a significant impact on obtaining the available nutrients from the soil by crops [28], and changes in soil pH also significantly affect the microbial activity in the soil [25,29]. This study demonstrated that long-term fertilization reduced the overall pH of the surface soil, which is consistent with the report by Chen et al. (2017) [9]. It is essential that soil has a certain pH range for the growth of crops. In this study, it was found that under fertilization, soil pH transforms to a range that is more suitable for crop growth and soil microbial growth, which is conducive to the accumulation of soil nutrients. The correlation matrix showed that soil pH is significantly correlated with SOC and TN content, which also confirms the above conclusion. In addition, although available soil nutrients can be directly used by crops as effective nutrients, in this study, we found that fertilization did not significantly change the available nutrients in soil. Fertilization had no significant effect on soil nutrient content at a soil depth of 20–40 cm. The principal reason is that the principal growth environment for the crop is the surface layer. Moreover, the crop root system is shallow; the soil structure and permeability are poor, and the environmental microbial activity is low in the lower soil layer. Therefore, fertilization has little effect on the nutrients in the deep soil. At the same time, the surface soil is greatly affected by the external environment and the return of litter nutrients; thus, the nutrients first gather in the surface soil and then migrate to the lower layer.

A lack of fertilization long term leads to pan class="Chemical">an increase in the soil nutrient content in the soil layer of 0–20 cm, which is inconsistent with the conclusion that a lack of fertilization long term leads to a decrease in soil orgpan class="Chemical">anic matter content [30]. It may be related to crop types and local environmental and climatic conditions. The soil nutrient content during the lack of fertilization long term exhibited a slightly increasing trend; the reason may be that during the entire growth cycle of the crop, its aboveground growth is vigorous, and the residual branches, leaves, and roots return to the soil through the humification process, which increases the soil nutrient content.

At a soil depth of 0–20 cm, the application of organic fertilizer or mixed apn>plication of organic and inorganic fertilizers had a more enhanced effect on soil nutrient content than the single application of inorganic fertilizer (Figure 3, Tables S2 and S3). This is because exogenous organic fertilizers can directly increase the C, N, and P content of the soil. In addition, long-term single application of inorganic fertilizers might not make the soil nutrients gradual and continuous. Although, to a certain extent, inorganic fertilizers can significantly increase crop yields. However, under the condition of no external organic fertilizer supplement, the long-term growth of crops will increase the consumption of nutrients in the soil, which will have a small increasing effect.

4.3. Long-Term Fertilization Altered C:N:P Stoichiometry in Soil

In this study, it was found that the SOC:pan class="Chemical">TN in 0–20 cm soil layer increased after fertilization with the increase in the number of years of fertilization. Studies have found that the main factors affecting the chpan class="Chemical">anges in SOC:TN are changes in SOC and soil TN content after fertilization [25,31]. SOC:TN in soil has a significant positive correlation with SOC and TN (Figure 7a). In this study, the overall rate of increase in SOC was greater than that of soil TN (Tables S2 and S3). Therefore, the SOC:TN in soil increased after fertilization. SOC:TN can reflect the decomposition rate of organic matter in the soil [32]. Therefore, we believe that the decomposition rate of soil organic matter after fertilization increases with the increase in the duration of fertilization, which is beneficial to the growth of crops. In addition, the overall SOC:TN in this study was lower than the average value in China and the world [33,34]. Under normal circumstances, lower SOC:TN can promote the vitality of soil microorganisms [35] and help in enhancing the conversion of nutrients in the soil.

SOC:pan class="Chemical">TP in soil cclass="Chemical">n>an class="Chemical">an characterize the potential of soil organic matter mineralization to release or absorb and fix P [25,32]. Additionally, this study reported that after fertilization, the rate of increase in SOC was greater than that in soil TP, resulting in an increase in the SOC:TP with the increase in the number of years of fertilization (Tables S2 and S4). In addition, the SOC:TP ratios in soil under all fertilization treatments were lower than the average value in China [34]. This may be because the soil nutrients in the arid and semi-arid areas are poor, and the microbial activity is low. To maintain the growth of vegetation, the circulation of soil nutrients must be improved so that the rate of SOC:TP is higher.

In addition, we found that the overall rate of increase in pan class="Chemical">TN was greater thpan class="Chemical">an that in TP under O and ON treatments (Tables S3 and S4), resulting in an increase in the TN:TP with increasing duration of fertilization. TN:TP is used as an index of soil nutrient restriction [34], and N and P are essential mineral nutrients and are common limiting elements for plant growth. In this study, we found that O and ON treatments had a higher TN:TP, which indicated that applying O or ON treatments can improve soil properties in a better way. However, the TN:TP in all fertilization treatments was at a low level, indicating that in arid and semi-arid regions, crop growth is limited by N for a long time. In addition, this study reported that long-term fertilization had little effect on the C:N:P stoichiometry of the 20–40 cm deep soil layer, which was mainly attributed to the lower change in SOC, TN, and TP.

5. Conclusions

In conclusion, we studied nine fertilization treatments for ppan class="Chemical">an class="Species">milletpan> in arid and semi-arid areas and found that crop yield and C:N:P stoichiometric ratio in soil were significanpan>tly affected by the duration of fertilization. The long-term use of organpan>ic fertilizer, inorganpan>ic fertilizer, or a combination of both canpan> effectively increase crop yield, improve soil nutrient environmental conditions, and improve C:N:P stoichiometry in 0–20 cm deep soil layer. The effect of organpan>ic fertilizer and combined application of organpan>ic and inorganpan>ic fertilizers on increasing soil nutrient content is more evident. Fertilization using organpan>ic fertilizer or organpan>ic fertilizer combined with an class="Chemical">nitrogen fertilizer can effectively alleviate nitrogen limitation in arid and semi-arid areas. Therefore, this study provided supportive data for changes in soil nutrient content and C:N:P stoichiometric ratio after long-term fertilization in arid and semi-arid regions and provided a theoretical basis for efficient soil fertilization in such areas.