Literature DB >> 33922494

Development of a Multiplex Bead Assay for Simultaneous Serodiagnosis of Antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis in Wild Boar.

Antonia Touloudi1, George Valiakos1, Shaun Cawthraw2, Polychronis Kostoulas1,3, Christian Gortázar4, Mariana Boadella5, Alexios Giannakopoulos1, Periklis Birtsas6, Marina Sofia1, Labrini V Athanasiou1, Maria Satra3, Zoi Athanasakopoulou1, Maria Kantere1, Vassiliki Spyrou7, Liljana Petrovska2, Charalambos Billinis1,3.   

Abstract

The aim of this study was to evaluate the diagnostic performance of a multiplex bead assay for the simultaneous detection of antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis. Sera from Eurasian wild boar of known serological status for TB (64 seropositive, 106 seronegative), Brucella (30 seropositive, 39 seronegative), and Trichinella (21 seropositive, 97 seronegative) were used for the development and evaluation of the assay. Magnetic beads coated with recombinant MPB83 antigen (TB), a whole-cell B. suis 1330 antigen, and an E/S T. spiralis antigen were used for the detection of specific antibodies using Bio-Rad Bio-Plex technology. The sensitivities (Se) and specificities (Sp) of the multiplex assay were, for M. bovis, 0.98 and 0.86; for B. suis, 1.00 and 0.97; and for T. spiralis, 0.90 and 0.99 (Se and Sp, respectively). The results show the diagnostic potential of this assay for the simultaneous detection of antibodies against M. bovis, B. suis, and T. spiralis in wild boar.

Entities:  

Keywords:  Brucella suis; Mycobacterium bovis; Trichinella spiralis; multiplex bead assay; serological assay; wild boar

Year:  2021        PMID： 33922494     DOI： 10.3390/microorganisms9050904

Source DB:  PubMed          Journal:  Microorganisms        ISSN： 2076-2607


  36 in total

1.  Multiplex bead array assays: performance evaluation and comparison of sensitivity to ELISA.

Authors:  Mohamed F Elshal; J Philip McCoy
Journal:  Methods       Date:  2006-04       Impact factor: 3.608

2.  Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife.

Authors:  K P Lyashchenko; R Greenwald; J Esfandiari; M A Chambers; J Vicente; C Gortazar; N Santos; M Correia-Neves; B M Buddle; R Jackson; D J O'Brien; S Schmitt; M V Palmer; R J Delahay; W R Waters
Journal:  Vet Microbiol       Date:  2008-07-07       Impact factor: 3.293

Review 3.  The Disease Ecology, Epidemiology, Clinical Manifestations, and Management of Trichinellosis Linked to Consumption of Wild Animal Meat.

Authors:  James H Diaz; Rebecca J Warren; Marissa J Oster
Journal:  Wilderness Environ Med       Date:  2020-03-10       Impact factor: 1.518

4.  Brucellosis in the United States: role and significance of wildlife reservoirs.

Authors:  S C Olsen
Journal:  Vaccine       Date:  2010-04-01       Impact factor: 3.641

5.  Evaluation of primary binding assays for presumptive serodiagnosis of swine brucellosis in Argentina.

Authors:  P S Paulo; A M Vigliocco; R F Ramondino; D Marticorena; E Bissi; G Briones; C Gorchs; D Gall; K Nielsen
Journal:  Clin Diagn Lab Immunol       Date:  2000-09

6.  Serologic tests for detecting antibodies against Mycobacterium bovis and Mycobacterium avium subspecies paratuberculosis in Eurasian wild boar (Sus scrofa scrofa).

Authors:  Mariana Boadella; Konstantin Lyashchenko; Reena Greenwald; Javan Esfandiari; Raquel Jaroso; Tania Carta; Joseba M Garrido; Joaquín Vicente; José de la Fuente; Christian Gortázar
Journal:  J Vet Diagn Invest       Date:  2011-01       Impact factor: 1.279

7.  Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses.

Authors:  Michael Coad; Derek Clifford; Shelley G Rhodes; R Glyn Hewinson; H Martin Vordermeier; Adam O Whelan
Journal:  Vet Res       Date:  2009-10-20       Impact factor: 3.683

8.  Serological detection of Trichinella spiralis in swine by ELISA (enzyme-linked immunosorbent assay) using an excretory-secretory (E/S) antigen.

Authors:  Karina Korínková; Kamil Kovarcík; Zdena Pavlícková; Martin Svoboda; Bretislav Koudela
Journal:  Parasitol Res       Date:  2008-02-17       Impact factor: 2.289

Review 9.  Evidence of the role of European wild boar as a reservoir of Mycobacterium tuberculosis complex.

Authors:  Victoria Naranjo; Christian Gortazar; Joaquín Vicente; José de la Fuente
Journal:  Vet Microbiol       Date:  2007-10-10       Impact factor: 3.293

10.  Field evaluation of the enzyme-linked immunosorbent assay for swine trichinosis: efficacy of the excretory-secretory antigen.

Authors:  K D Murrell; W R Anderson; G A Schad; R D Hanbury; K R Kazacos; H R Gamble; J Brown
Journal:  Am J Vet Res       Date:  1986-05       Impact factor: 1.156
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  1 in total

1.  Development of a Multiplex Bead Assay to Detect Serological Responses to Brucella Species in Domestic Pigs and Wild Boar with the Potential to Overcome Cross-Reactivity with Yersinia enterocolitica O:9.

Authors:  Antonia Touloudi; John McGiven; Shaun Cawthraw; George Valiakos; Polychronis Kostoulas; Lucy Duncombe; Christian Gortázar; Mariana Boadella; Marina Sofia; Zoi Athanasakopoulou; Dimitris C Chatzopoulos; Vassiliki Spyrou; Liljana Petrovska; Charalambos Billinis
Journal:  Microorganisms       Date:  2022-07-06
  1 in total

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