Chunwei Yu1, Yuxiang Ji1, Shaobai Wen1, Jun Zhang1,2. 1. Laboratory of Environmental Monitoring, School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou 571101, China. 2. Laboratory of Tropical Biomedicine and Biotechnology, Hainan Medical University, Haikou 571101, China.
Abstract
A simple benzoyl hydrazine derivative P was successfully synthesized and characterized as Mg2+-selective fluorescent probe. The binding of P with Mg2+ caused an obvious fluorescence enhancement at 482 nm. The fluorescent, UV-vis spectra, 1H-NMR, and IR spectra confirmed the formation of P-Mg2+ complex, and the formation of a 1:1 stoichiometry complex was proved by Job's plot and mass spectrometry. The recognition mechanism of P to Mg2+ was owing to the photoinduced electron transfer effect (PET). The fluorescent response was linear in the range of 0.9-4.0 µM with the detection limit of 0.3 µM Mg2+ in water-ethanol solution (1:9, v:v, pH10.0, 20 mM HEPES). In addition, the results of cell imaging of Mg2+ in Hl-7701 cells was satisfying.
A simple class="Chemical">benzoyl hydrazine derivative P was successfully synthesized and characterized as class="Chemical">pan class="Chemical">Mg2+-selective fluorescent probe. The binding of P with Mg2+ caused an obvious fluorescence enhancement at 482 nm. The fluorescent, UV-vis spectra, 1H-NMR, and IR spectra confirmed the formation of P-Mg2+ complex, and the formation of a 1:1 stoichiometry complex was proved by Job's plot and mass spectrometry. The recognition mechanism of P to Mg2+ was owing to the photoinduced electron transfer effect (PET). The fluorescent response was linear in the range of 0.9-4.0 µM with the detection limit of 0.3 µM Mg2+ in water-ethanol solution (1:9, v:v, pH10.0, 20 mM HEPES). In addition, the results of cell imaging of Mg2+ in Hl-7701 cells was satisfying.
Authors: Adam C Sedgwick; Luling Wu; Hai-Hao Han; Steven D Bull; Xiao-Peng He; Tony D James; Jonathan L Sessler; Ben Zhong Tang; He Tian; Juyoung Yoon Journal: Chem Soc Rev Date: 2018-11-26 Impact factor: 54.564
Authors: Sarah E Huff; Faiz Ahmad Mohammed; Mu Yang; Prashansa Agrawal; John Pink; Michael E Harris; Chris G Dealwis; Rajesh Viswanathan Journal: J Med Chem Date: 2018-01-05 Impact factor: 7.446