Ping Huang1, Lili He1, Adeel Abbas1, Sadam Hussain2, Saddam Hussain3, Daolin Du1, Muhammad Bilal Hafeez2,3, Sidra Balooch4, Noreen Zahra5, Xiaolong Ren2, Muhammad Rafiq6, Muhammad Saqib6. 1. Institute of Environment and Ecology, School of Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China. 2. College of Agronomy, Northwest A&F University, Yangling 712100, China. 3. Department of Agronomy, University of Agriculture, Faisalabad 38040, Pakistan. 4. Department of Botany, Ghazi University D.G, Khan 32200, Pakistan. 5. Department of Botany, University of Agriculture, Faisalabad 38040, Pakistan. 6. Agronomic Research Institute, Ayub Agricultural Research Institute, Faisalabad 38040, Pakistan.
Abstract
Seed priming with sorghum water extract (SWE) enhances crop tolerance to salinity stress; however, the application of SWE under salinity for camelina crop has not been documented so far. This study evaluated the potential role of seed priming with SWE in improving salt stress tolerance in camelina. Primed (with 5% SWE and distilled water-hydropriming) and nonprimed seeds were sown under control (no salt) and salt stress (10 dS m-1) conditions. Salinity reduced camelina's emergence and growth, while seed priming with SWE improved growth under control and stress conditions. Under salt stress, seed priming with SWE enhanced emergence percentage (96.98%), increased root length (82%), shoot length (32%), root dry weight (75%), shoot dry weight (33%), α-amylase activity (66.43%), chlorophyll content (60-92%), antioxidant enzymes activity (38-171%) and shoot K+ ion (60%) compared with nontreated plants. Similarly, under stress conditions, hydrogen peroxide, malondialdehyde (MDA) content, and shoot Na+ ion were reduced by 60, 31, and 40% by seed priming with SWE, respectively, over the nonprimed seeds. Therefore, seed priming with SWE may be used to enhance the tolerance against salt stress in camelina.
Seed priming with sorghumn>an class="Chemical">water extract (SWE) enhances crop tolerance to salinity stress; however, the application of SWE under salinity for camelina crop has not been documented so far. This study evaluated the potential role of seed priming with SWE in improving saltstress tolerance in camelina. Primed (with 5% SWE and distilled water-hydropriming) and nonprimed seeds were sown under control (no salt) and saltstress (10 dS m-1) conditions. Salinity reduced camelina's emergence and growth, while seed priming with SWE improved growth under control and stress conditions. Under saltstress, seed priming with SWE enhanced emergence percentage (96.98%), increased root length (82%), shoot length (32%), root dry weight (75%), shoot dry weight (33%), α-amylase activity (66.43%), chlorophyll content (60-92%), antioxidant enzymes activity (38-171%) and shoot K+ ion (60%) compared with nontreated plants. Similarly, under stress conditions, hydrogen peroxide, malondialdehyde (MDA) content, and shoot Na+ ion were reduced by 60, 31, and 40% by seed priming with SWE, respectively, over the nonprimed seeds. Therefore, seed priming with SWE may be used to enhance the tolerance against saltstress in camelina.