| Literature DB >> 33918386 |
Ana Klobucar1, Vladimir Savic2, Marcela Curman Posavec1, Suncica Petrinic1, Urska Kuhar3, Ivan Toplak3, Josip Madic4, Tatjana Vilibic-Cavlek5,6.
Abstract
In the period from 2015 to 2020, an entomological survey for the presence of West Nile virus (WNV) and Usutu virus (USUV) in mosquitoes was performed in northwestern Croatia. A total of 20,363 mosquitoes were sampled in the City of Zagreb and Međimurje county, grouped in 899 pools and tested by real-time RT-PCR for WNV and USUV RNA. All pools were negative for WNV while one pool each from 2016 (Aedes albopictus), 2017 (Culex pipiens complex), 2018 (Cx. pipiens complex), and 2019 (Cx. pipiens complex), respectively, was positive for USUV. The 2018 and 2019 positive pools shared 99.31% nucleotide homology within the USUV NS5 gene and both clustered within USUV Europe 2 lineage. The next-generation sequencing of one mosquito pool (Cx. pipiens complex) collected in 2018 in Zagreb confirmed the presence of USUV and revealed several dsDNA and ssRNA viruses of insect, bacterial and mammalian origin.Entities:
Keywords: Croatia; Usutu virus; West Nile virus; mosquitoes
Year: 2021 PMID： 33918386 DOI： 10.3390/tropicalmed6020045
Source DB: PubMed Journal: Trop Med Infect Dis ISSN： 2414-6366