Vasileios Papanikolaou1, Aristeidis Chrysovergis1, Stylianos Mastronikolis2, Evangelos Tsiambas3, Vasileios Ragos4, Dimitrios Peschos5, Despoina Spyropoulou6, Pavlos Pantos1, Athanasios Niotis7, Nicholas Mastronikolis8, Efthymios Kyrodimos1. 1. 1 ENT Department, Hippocration Hospital, University of Athens, Athens, Greece. 2. Medical School, University of Heraklion, Heraklion, Greece. 3. Department of Cytology, 417 VA Hospital (NIMTS), Athens, Greece; tsiambasecyto@yahoo.gr. 4. Department of Maxillofacial Surgery, Medical School, University of Ioannina, Ioannina, Greece. 5. Department of Physiology, Medical School, University of Ioannina, Ioannina, Greece. 6. Department of Radiation Oncology, Medical School, University of Patras, Patras, Greece. 7. Department of Surgery, 417 VA Hospital (NIMTS), Athens, Greece. 8. ENT Department, Medical School, University of Patras, Patras, Greece.
Abstract
BACKGROUND/AIM: Oncogene up-regulation combined with suppressor gene down-regulation is a crucial genetic combination that promotes cell neoplastic phenotype and progressively malignant transformation in solid malignancies, including laryngeal squamous cell carcinoma (LSCC). Among oncogenes, the Kirsten ras oncogene homolog (K-Ras) is involved in LSCC onset and progression. PATIENTS AND METHODS: Sixty (n=60) primary LSCC tissue sections were analyzed by immunohistochemistry (IHC). Digital image analysis (DIA) was also implemented for measuring K-Ras protein expression levels. RESULTS: High K-Ras protein expression levels were observed in 20/60 (33.3%) LSCC tissue sections, whereas the rest of the cases (n=40; 66.7%) demonstrated low expression. Overall K-Ras expression was borderline significantly associated to the grade of the examined malignancies (p=0.048), whereas no other strong statistical correlations were identified. A progressive K-Ras overexpression was observed in all grades of the examined cases. CONCLUSION: K-Ras over expression is correlated to a progressive dedifferentiation in LSCC. Copyright
BACKGROUND/AIM: Oncogene up-regulation combined with suppressor gene down-regulation is a crucial genetic combination that promotes cell neoplastic phenotype and progressively malignant transformation in solid malignancies, including laryngeal squamous cell carcinoma (LSCC). Among oncogenes, the Kirsten ras oncogene homolog (K-Ras) is involved in LSCC onset and progression. PATIENTS AND METHODS: Sixty (n=60) primary LSCC tissue sections were analyzed by immunohistochemistry (IHC). Digital image analysis (DIA) was also implemented for measuring K-Ras protein expression levels. RESULTS: High K-Ras protein expression levels were observed in 20/60 (33.3%) LSCC tissue sections, whereas the rest of the cases (n=40; 66.7%) demonstrated low expression. Overall K-Ras expression was borderline significantly associated to the grade of the examined malignancies (p=0.048), whereas no other strong statistical correlations were identified. A progressive K-Ras overexpression was observed in all grades of the examined cases. CONCLUSION: K-Ras over expression is correlated to a progressive dedifferentiation in LSCC. Copyright
Authors: Anastasia Politi; Evangelos Tsiambas; Nicholas S Mastronikolis; Dimitrios Peschos; Ioannis Asproudis; Efthymios Kyrodimos; Ilianna E Armata; Aristeidis Chrysovergis; Asimakis Asimakopoulos; Vasileios S Papanikolaou; Anna Batistatou; Vasileios Ragos Journal: In Vivo Date: 2019 May-Jun Impact factor: 2.155
Authors: Helena Linardou; Vassiliki Kotoula; George Kouvatseas; Giannis Mountzios; Vasilios Karavasilis; Epaminondas Samantas; Anna Kalogera-Fountzila; Despina Televantou; Kyriaki Papadopoulou; Xanthipi Mavropoulou; Emily Daskalaki; Thomas Zaramboukas; Ioannis Efstratiou; Sofia Lampaki; Grigorios Rallis; Eleni Res; Konstantinos N Syrigos; Paris A Kosmidis; Dimitrios Pectasides; George Fountzilas Journal: Cancer Genomics Proteomics Date: 2019 Nov-Dec Impact factor: 4.069