A-Rang Lee1, Su-Min Baek1, Seoung-Woo Lee1, Tae-Un Kim1, Jee Eun Han2, Seulgi Bae2, Sang-Joon Park2, Tae-Hwan Kim1, Kyu-Shik Jeong1,3, Seong-Kyoon Choi4, Jin-Kyu Park5,3. 1. Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea. 2. College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea. 3. Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea. 4. Core Protein Resources Center, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, Republic of Korea jinkyu820@knu.ac.kr cskbest@dgist.ac.kr. 5. Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea; jinkyu820@knu.ac.kr cskbest@dgist.ac.kr.
Abstract
BACKGROUND/AIM: The pathological role of vascular endothelial growth factor receptor 2 (VEGFR-2) in chronic liver injury and liver regeneration is not fully understood. This study analysed the role of VEGFR-2 in liver fibrosis and its regeneration process. MATERIALS AND METHODS: We administered intraperitoneally 50 mg/kg to 300 mg/kg thioacetamide (TAA) to 9-week-old male mice for 17 weeks. We measured levels of VEGFR-2 protein and identified the location of cells that specifically express VEGFR-2. RESULTS: VEGFR-2 is rarely expressed in normal hepatocytes. However, high VEGFR-2 expression in liver sinusoidal endothelial cells was noted in the TAA group. Conversely, the group that experienced regeneration from liver fibrosis showed significantly higher VEGFR-2 expression in the nucleus of hepatocytes compared to the other groups. CONCLUSION: VEGFR-2 plays a pivotal role in the nucleus of hepatocytes during liver regeneration and VEGFR-2 may be closely related to cell division. Therefore, VEGFR-2 may be a new therapeutic target for liver regeneration. Copyright
BACKGROUND/AIM: The pathological role of vascular endothelial growth factor receptor 2 (VEGFR-2) in chronic liver injury and liver regeneration is not fully understood. This study analysed the role of VEGFR-2 in liver fibrosis and its regeneration process. MATERIALS AND METHODS: We administered intraperitoneally 50 mg/kg to 300 mg/kg thioacetamide (TAA) to 9-week-old male mice for 17 weeks. We measured levels of VEGFR-2 protein and identified the location of cells that specifically express VEGFR-2. RESULTS: VEGFR-2 is rarely expressed in normal hepatocytes. However, high VEGFR-2 expression in liver sinusoidal endothelial cells was noted in the TAA group. Conversely, the group that experienced regeneration from liver fibrosis showed significantly higher VEGFR-2 expression in the nucleus of hepatocytes compared to the other groups. CONCLUSION: VEGFR-2 plays a pivotal role in the nucleus of hepatocytes during liver regeneration and VEGFR-2 may be closely related to cell division. Therefore, VEGFR-2 may be a new therapeutic target for liver regeneration. Copyright
Authors: Hien T T Duong; Zhixia Dong; Lin Su; Cyrille Boyer; Jacob George; Thomas P Davis; Jianhua Wang Journal: Small Date: 2015-01-13 Impact factor: 13.281
Authors: P C Rensen; L A Sliedregt; M Ferns; E Kieviet; S M van Rossenberg; S H van Leeuwen; T J van Berkel; E A Biessen Journal: J Biol Chem Date: 2001-07-30 Impact factor: 5.157