Genes involved in disease resistance are some of the fastest evolving and most diverse components of genomes. Large numbers of nucleotide-binding, leucine-rich repeat (NLR) genes are found in plant genomes and are required for disease resistance. However, NLRs can trigger autoimmunity, disrupt beneficial microbiota or reduce fitness. It is therefore crucial to understand how NLRs are controlled. Here, we show that the RNA-binding protein FPA mediates widespread premature cleavage and polyadenylation of NLR transcripts, thereby controlling their functional expression and impacting immunity. Using long-read Nanopore direct RNA sequencing, we resolved the complexity of NLR transcript processing and gene annotation. Our results uncover a co-transcriptional layer of NLR control with implications for understanding the regulatory and evolutionary dynamics of NLRs in the immune responses of plants.
Genes involved in disease resistance are some of the fastest evolving and most diverse components of genomes. Large numbers of nucleotide-binding, leucine-rich repeat (NLR) genes are found in plant genomes and are required for disease resistance. However, NLRs can trigger autoimmunity, disrupt beneficial microbiota or reduce pan class="Disease">fitness. It is therefore crucial to understand how NLRs are controlled. Here, we show that the RNA-binding protein FPA mediates widespread premature cleavage and polyadenylation of NLR transcripts, thereby controlling their functional expression and impacting immunity. Using long-read Nanopore direct RNA sequencing, we resolved the complexity of NLR transcript processing and gene annotation. Our results uncover a co-transcriptional layer of NLR control with implications for understanding the regulatory and evolutionary dynamics of NLRs in the immune responses of plants.
Authors: Fatih Ozsolak; Adam R Platt; Dan R Jones; Jeffrey G Reifenberger; Lauryn E Sass; Peter McInerney; John F Thompson; Jayson Bowers; Mirna Jarosz; Patrice M Milos Journal: Nature Date: 2009-09-23 Impact factor: 49.962
Authors: Zheng Xia; Lawrence A Donehower; Thomas A Cooper; Joel R Neilson; David A Wheeler; Eric J Wagner; Wei Li Journal: Nat Commun Date: 2014-11-20 Impact factor: 14.919
Authors: Philip Knuckles; Tina Lence; Irmgard U Haussmann; Dominik Jacob; Nastasja Kreim; Sarah H Carl; Irene Masiello; Tina Hares; Rodrigo Villaseñor; Daniel Hess; Miguel A Andrade-Navarro; Marco Biggiogera; Mark Helm; Matthias Soller; Marc Bühler; Jean-Yves Roignant Journal: Genes Dev Date: 2018-03-13 Impact factor: 11.361
Authors: Xuhong Yu; Pascal G P Martin; Yixiang Zhang; Jonathan C Trinidad; Feifei Xu; Jie Huang; Karen E Thum; Ke Li; ShuZhen Zhao; Yangnan Gu; Xingjun Wang; Scott D Michaels Journal: Curr Biol Date: 2021-10-18 Impact factor: 10.834
Authors: Runxuan Zhang; Richard Kuo; Max Coulter; Cristiane P G Calixto; Juan Carlos Entizne; Wenbin Guo; Yamile Marquez; Linda Milne; Stefan Riegler; Akihiro Matsui; Maho Tanaka; Sarah Harvey; Yubang Gao; Theresa Wießner-Kroh; Alejandro Paniagua; Martin Crespi; Katherine Denby; Asa Ben Hur; Enamul Huq; Michael Jantsch; Artur Jarmolowski; Tino Koester; Sascha Laubinger; Qingshun Quinn Li; Lianfeng Gu; Motoaki Seki; Dorothee Staiger; Ramanjulu Sunkar; Zofia Szweykowska-Kulinska; Shih-Long Tu; Andreas Wachter; Robbie Waugh; Liming Xiong; Xiao-Ning Zhang; Ana Conesa; Anireddy S N Reddy; Andrea Barta; Maria Kalyna; John W S Brown Journal: Genome Biol Date: 2022-07-07 Impact factor: 17.906
Authors: Mi Zhang; Zsuzsanna Bodi; Katarzyna Mackinnon; Silin Zhong; Nathan Archer; Nigel P Mongan; Gordon G Simpson; Rupert G Fray Journal: Nat Commun Date: 2022-03-02 Impact factor: 14.919