Eleesha Sana1, Mahira Zeeshan1, Qurat Ul Ain1, Ashraf Ullah Khan1, Irshad Hussain2, Salman Khan1, Elise Lepeltier3, Hussain Ali1. 1. Department of Pharmacy, Quaid-i-Azam University, Islamabad 45320, Pakistan. 2. Department of Chemistry & Chemical Engineering, SBA School of Science & Engineering, Lahore University of Management Sciences, Lahore Cantt 54792, Pakistan. 3. MINT, UNIV Angers, INSERM 1066, CNRS, Angers 6021, France.
Abstract
Aim: To fabricate and evaluate curcumin-loaded transfersomes (Cur-TF) for the targeted delivery and enhanced therapeutic efficacy of curcumin for the treatment of rheumatoid arthritis (RA). Methods: Modified thin-film hydration method was used to prepare Cur-TF which were then embedded into carbopol-934 gel. They were further evaluated through in vitro techniques and in an in vivo arthritis model. Results: Cur-TF had optimal particle size, spherical morphology, high encapsulation efficiency and sustained drug release profiles. The Cur-TF gel had better in vitro skin penetration than plain curcumin. In vivo findings demonstrated improved clinical, histological and x-ray scores and reduced pro-inflammatory cytokines through NF-κβ inhibition. Conclusion: Cur-TF gel delivered curcumin to the arthritic dermal tissue through a topical route and demonstrated promising therapeutic efficacy by significantly alleviating complete Freud's adjuvant (CFA)-induced arthritis.
Aim: To fabricate and evaluate curcumin-loaded transfersomes (Cur-TF) for the targeted delivery and enhanced therapeutic efficacy of curcumin for the treatment of rheumatoid arthritis (RA). Methods: Modified thin-film hydration method was used to prepare Cur-TF which were then embedded into carbopol-934 gel. They were further evaluated through in vitro techniques and in an in vivo arthritis model. Results: Cur-TF had optimal particle size, spherical morphology, high encapsulation efficiency and sustained drug release profiles. The Cur-TF gel had better in vitro skin penetration than plain curcumin. In vivo findings demonstrated improved clinical, histological and x-ray scores and reduced pro-inflammatory cytokines through NF-κβ inhibition. Conclusion: Cur-TF gel delivered curcumin to the arthritic dermal tissue through a topical route and demonstrated promising therapeutic efficacy by significantly alleviating complete Freud's adjuvant (CFA)-induced arthritis.
Authors: Md Abdullah Shamim; Steven Yeung; Ayaz Shahid; Mengbing Chen; Jeffrey Wang; Preshita Desai; Cyrus Parsa; Robert Orlando; Frank L Meyskens; Kristen M Kelly; Bradley T Andresen; Ying Huang Journal: Int J Pharm Date: 2021-11-15 Impact factor: 5.875
Authors: Mirosław Zagaja; Anna Zagaja; Joanna Szala-Rycaj; Aleksandra Szewczyk; Marta Kinga Lemieszek; Grzegorz Raszewski; Marta Andres-Mach Journal: Int J Mol Sci Date: 2022-03-23 Impact factor: 5.923