Sabrina Carvalho Gomes1,2, Sabrina Fachin3, Juliane Gonçalves da Fonseca3, Patrícia Daniela Melchiors Angst1,2, Marcelo Lazzaron Lamers4, Ilma Simoni Brum da Silva5, Luciana Neves Nunes6. 1. Department of Conservative Dentistry, Dentistry School, Federal University of Rio Grande do Sul, Porto Alegre, Brazil. 2. Hospital de Clínicas de Porto Alegre, Federal University of Rio Grande do Sul, Porto Alegre, Brazil. 3. Resident in Periodontology, Dental Faculty, Federal University of Rio Grande do Sul, Porto Alegre, Brazil. 4. Department of Morphological Sciences, Dentistry School, Federal University of Rio Grande do Sul, Porto Alegre, Brazil. 5. Department of Physiology, Basic Health Science Institute, Federal University of Rio Grande do Sul, Porto Alegre, Brazil. 6. Mathematics and Statistics Institute, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
Abstract
AIMS: SARS-CoV-2 RNA has been recovered from different sites in the human body, including the mouth. The present study aimed to investigate the presence of SARS-CoV-2 RNA in the dental biofilm of symptomatic patients who tested positive in nasopharyngeal and oropharyngeal (NASO/ORO) samples. MATERIALS & METHODS: An observational clinical study of individuals with flu-like symptoms was conducted between July and September 2020. Dental biofilm (BIO) samples were collected and analysed using real-time quantitative polymerase chain reaction (RT-qPCR) to determine the virus's presence. RESULTS: Seventy participants (40 ± 9.8 years of age, 71.4% female) tested positive for SARS-CoV-2 RNA in NASO/ORO samples and were included in the study. Among them, 13 tested positive in BIO samples (18.6%; 95% CI: [9.5, 27.7]). The median and interquartile range of cycle quantification (Cq) for NASO/ORO and BIO samples were 15.9 [6.9] and 35.9 [4.0] (p = .001), respectively. BIO-positive participants showed a higher virus load in NASO/ORO samples (p = .012) than those testing negative (Cq = 20.4 [6.1]). CONCLUSIONS: Dental biofilms from symptomatic COVID-19 patients harbour SARS-CoV-2 RNA and might be a potential reservoir with an essential role in COVID-19 transmission.
AIMS: SARS-CoV-2 RNA has been recovered from different sites in the human body, including the mouth. The present study aimed to investigate the presence of SARS-CoV-2 RNA in the dental biofilm of symptomatic patients who tested positive in nasopharyngeal and oropharyngeal (NASO/ORO) samples. MATERIALS & METHODS: An observational clinical study of individuals with flu-like symptoms was conducted between July and September 2020. Dental biofilm (BIO) samples were collected and analysed using real-time quantitative polymerase chain reaction (RT-qPCR) to determine the virus's presence. RESULTS: Seventy participants (40 ± 9.8 years of age, 71.4% female) tested positive for SARS-CoV-2 RNA in NASO/ORO samples and were included in the study. Among them, 13 tested positive in BIO samples (18.6%; 95% CI: [9.5, 27.7]). The median and interquartile range of cycle quantification (Cq) for NASO/ORO and BIO samples were 15.9 [6.9] and 35.9 [4.0] (p = .001), respectively. BIO-positive participants showed a higher virus load in NASO/ORO samples (p = .012) than those testing negative (Cq = 20.4 [6.1]). CONCLUSIONS: Dental biofilms from symptomatic COVID-19patients harbour SARS-CoV-2 RNA and might be a potential reservoir with an essential role in COVID-19 transmission.
Authors: Livia Barenghi; Alberto Barenghi; Umberto Garagiola; Alberto Di Blasio; Aldo Bruno Giannì; Francesco Spadari Journal: Sensors (Basel) Date: 2021-12-22 Impact factor: 3.576
Authors: Jhon Paul Iakov Mezarina Mendoza; Briggitte Patricia Trelles Ubillús; Gabriela Tazziana Salcedo Bolívar; Rosa Del Pilar Castañeda Palacios; Paulo Sergio Gilmar Herrera Lopez; David Alex Padilla Rodríguez; Karin Harumi Uchima Koecklin Journal: Saudi Dent J Date: 2022-02-01
Authors: Sabrina C Gomes; Juliane G da Fonseca; Luísa M Miller; Luciane Manenti; Patrícia Daniela M Angst; Marcelo L Lamers; Ilma S Brum; Luciana N Nunes Journal: J Periodontol Date: 2022-03-03 Impact factor: 4.494