Ghasem Yazdanpanah1, Ritu Shah2, Sri Raghurama R Somala2, Khandaker N Anwar2, Xiang Shen2, Seungwon An2, Meisam Omidi3, Mark I Rosenblatt2, Tolou Shokuhfar4, Ali R Djalilian5. 1. Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago, Chicago, IL, USA; Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, USA. 2. Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago, Chicago, IL, USA. 3. Marquette University School of Dentistry, Milwaukee, WI, USA. 4. Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, USA. 5. Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago, Chicago, IL, USA. Electronic address: adjalili@uic.edu.
Abstract
PURPOSE: Bioactive substrates can be used therapeutically to enhance wound healing. Here, we evaluated the effect of an in-situ thermoresponsive hydrogel from decellularized porcine cornea ECM, COMatrix (COrnea Matrix), for application as an ocular surface bandage for corneal epithelial defects. METHODS: COMatrix hydrogel was fabricated from decellularized porcine corneas. The effects of COMatrix hydrogel on attachment and proliferation of human corneal epithelial cells (HCECs) were evaluated in vitro. The effect of COMatrix on the expressions of the inflammatory genes, IL-1β, TNF-α, and IL-6 was assessed by RT-PCR. The in-situ application and also repairing effects of COMatrix hydrogel as an ocular bandage was studied in a murine model of corneal epithelial wound. The eyes were examined by optical coherence tomography (OCT) and slit-lamp microscopy in vivo and by histology and immunofluorescence post-mortem. RESULTS: In vitro, COMatrix hydrogel significantly enhanced the attachment and proliferation of HCECs relative to control. HCECs exposed to COMatrix had less induced expression of TNF-α (P < 0.05). In vivo, COMatrix formed a uniform hydrogel that adhered to the murine ocular surface after in-situ curing. Corneal epithelial wound closure was significantly accelerated by COMatrix hydrogel compared to control (P < 0.01). There was significant increase in the expression of proliferation marker Ki-67 in wounded corneal epithelium by COMatrix hydrogel compared to control (P < 0.05). CONCLUSIONS: COMatrix hydrogel is a naturally derived bioactive material with potential application as an ocular surface bandage to enhance epithelial wound healing.
PURPOSE: Bioactive substrates can be used therapeutically to enhance wound healing. Here, we evaluated the effect of an in-situ thermoresponsive hydrogel from decellularized porcine cornea ECM, COMatrix (COrnea Matrix), for application as an ocular surface bandage for corneal epithelial defects. METHODS: COMatrix hydrogel was fabricated from decellularized porcine corneas. The effects of COMatrix hydrogel on attachment and proliferation of human corneal epithelial cells (HCECs) were evaluated in vitro. The effect of COMatrix on the expressions of the inflammatory genes, IL-1β, TNF-α, and IL-6 was assessed by RT-PCR. The in-situ application and also repairing effects of COMatrix hydrogel as an ocular bandage was studied in a murine model of corneal epithelial wound. The eyes were examined by optical coherence tomography (OCT) and slit-lamp microscopy in vivo and by histology and immunofluorescence post-mortem. RESULTS: In vitro, COMatrix hydrogel significantly enhanced the attachment and proliferation of HCECs relative to control. HCECs exposed to COMatrix had less induced expression of TNF-α (P < 0.05). In vivo, COMatrix formed a uniform hydrogel that adhered to the murine ocular surface after in-situ curing. Corneal epithelial wound closure was significantly accelerated by COMatrix hydrogel compared to control (P < 0.01). There was significant increase in the expression of proliferation marker Ki-67 in wounded corneal epithelium by COMatrix hydrogel compared to control (P < 0.05). CONCLUSIONS: COMatrix hydrogel is a naturally derived bioactive material with potential application as an ocular surface bandage to enhance epithelial wound healing.
Authors: Lindsey T Saldin; Madeline C Cramer; Sachin S Velankar; Lisa J White; Stephen F Badylak Journal: Acta Biomater Date: 2016-12-01 Impact factor: 8.947
Authors: Hyeon Ii Lee; Mee Kum Kim; Joo Youn Oh; Jung Hwa Ko; Hyun Ju Lee; Won Ryang Wee; Jin Hak Lee Journal: Xenotransplantation Date: 2007-11 Impact factor: 3.907
Authors: Gabriella Maria Fernandes-Cunha; Kyung-Sun Na; Ilham Putra; Hyun Jong Lee; Sarah Hull; Yu-Chia Cheng; Ignacio Jesus Blanco; Medi Eslani; Ali R Djalilian; David Myung Journal: Stem Cells Transl Med Date: 2019-01-15 Impact factor: 6.940
Authors: Gabriella Maria Fernandes-Cunha; Karen Mei Chen; Fang Chen; Peter Le; Ju Hee Han; Leela Ann Mahajan; Hyun Jong Lee; Kyung Sun Na; David Myung Journal: Sci Rep Date: 2020-10-07 Impact factor: 4.996
Authors: Sina Sharifi; Hannah Sharifi; Ali Akbari; Fengyang Lei; Claes H Dohlman; Miguel Gonzalez-Andrades; Curtis Guild; Eleftherios I Paschalis; James Chodosh Journal: Acta Biomater Date: 2021-10-28 Impact factor: 8.947
Authors: Ghasem Yazdanpanah; Yizhou Jiang; Behnam Rabiee; Meisam Omidi; Mark I Rosenblatt; Tolou Shokuhfar; Yayue Pan; Alexandra Naba; Ali R Djalilian Journal: Tissue Eng Part C Methods Date: 2021-05 Impact factor: 3.056
Authors: Abdelrahman M Elhusseiny; Mohammad Soleimani; Taher K Eleiwa; Reem H ElSheikh; Charles R Frank; Morteza Naderan; Ghasem Yazdanpanah; Mark I Rosenblatt; Ali R Djalilian Journal: Stem Cells Transl Med Date: 2022-03-31 Impact factor: 6.940
Authors: Sohil Amin; Elmira Jalilian; Eitan Katz; Charlie Frank; Ghasem Yazdanpanah; Victor H Guaiquil; Mark I Rosenblatt; Ali R Djalilian Journal: Vision (Basel) Date: 2021-09-22